Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13825 and LU632_RS13830 overlap by 2 nucleotides LU632_RS13830 and LU632_RS13835 are separated by 397 nucleotides LU632_RS13835 and fliD are separated by 280 nucleotides
LU632_RS13825: LU632_RS13825 - glycosyltransferase, at 2,650,877 to 2,654,404
_RS13825
LU632_RS13830: LU632_RS13830 - hypothetical protein, at 2,654,403 to 2,654,546
_RS13830
LU632_RS13835: LU632_RS13835 - FliC/FljB family flagellin, at 2,654,944 to 2,656,227
_RS13835
LU632_RS13840: fliD - flagellar filament capping protein FliD, at 2,656,508 to 2,657,920
fliD
Position (kb)
2654
2655
2656
2657 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2653.947 kb on - strand, within LU632_RS13825 at 2653.970 kb on + strand, within LU632_RS13825 at 2653.970 kb on + strand, within LU632_RS13825 at 2654.075 kb on - strand at 2654.075 kb on - strand at 2654.149 kb on - strand at 2654.149 kb on - strand at 2654.149 kb on - strand at 2654.149 kb on - strand at 2654.209 kb on + strand at 2654.209 kb on + strand at 2654.210 kb on - strand at 2654.213 kb on + strand at 2654.213 kb on + strand at 2654.213 kb on + strand at 2654.214 kb on - strand at 2654.214 kb on - strand at 2654.313 kb on + strand at 2654.313 kb on + strand at 2654.313 kb on + strand at 2654.314 kb on - strand at 2654.352 kb on + strand at 2654.353 kb on - strand at 2654.375 kb on - strand at 2654.472 kb on + strand, within LU632_RS13830 at 2654.473 kb on - strand, within LU632_RS13830 at 2654.516 kb on + strand, within LU632_RS13830 at 2654.551 kb on + strand at 2654.602 kb on - strand at 2654.657 kb on - strand at 2654.759 kb on + strand at 2654.793 kb on - strand at 2654.942 kb on + strand at 2655.006 kb on + strand at 2655.007 kb on - strand at 2655.127 kb on - strand, within LU632_RS13835 at 2655.174 kb on + strand, within LU632_RS13835 at 2655.175 kb on - strand, within LU632_RS13835 at 2655.229 kb on - strand, within LU632_RS13835 at 2655.237 kb on - strand, within LU632_RS13835 at 2655.349 kb on + strand, within LU632_RS13835 at 2655.499 kb on + strand, within LU632_RS13835 at 2655.500 kb on - strand, within LU632_RS13835 at 2655.500 kb on - strand, within LU632_RS13835 at 2655.507 kb on - strand, within LU632_RS13835 at 2655.507 kb on - strand, within LU632_RS13835 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.962 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2656.015 kb on - strand, within LU632_RS13835 at 2656.230 kb on + strand at 2656.259 kb on + strand at 2656.388 kb on + strand at 2656.433 kb on + strand at 2656.433 kb on + strand at 2656.471 kb on + strand at 2656.471 kb on + strand at 2656.508 kb on - strand at 2656.518 kb on + strand at 2656.702 kb on + strand, within fliD at 2656.786 kb on + strand, within fliD at 2656.790 kb on + strand, within fliD at 2656.791 kb on - strand, within fliD at 2656.888 kb on + strand, within fliD at 2656.889 kb on - strand, within fliD at 2657.005 kb on + strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.031 kb on - strand, within fliD at 2657.218 kb on + strand, within fliD
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,653,947 - LU632_RS13825 0.87 -4.0 2,653,970 + LU632_RS13825 0.88 +0.4 2,653,970 + LU632_RS13825 0.88 -0.1 2,654,075 - +1.9 2,654,075 - +0.1 2,654,149 - -0.7 2,654,149 - -0.6 2,654,149 - +1.2 2,654,149 - -0.0 2,654,209 + +0.0 2,654,209 + -0.7 2,654,210 - -1.1 2,654,213 + +0.2 2,654,213 + +0.9 2,654,213 + -0.5 2,654,214 - +0.3 2,654,214 - -0.3 2,654,313 + -0.2 2,654,313 + +0.5 2,654,313 + -0.1 2,654,314 - -0.1 2,654,352 + +0.1 2,654,353 - +0.3 2,654,375 - +0.6 2,654,472 + LU632_RS13830 0.48 -0.6 2,654,473 - LU632_RS13830 0.49 -1.1 2,654,516 + LU632_RS13830 0.78 +0.4 2,654,551 + -0.4 2,654,602 - +0.1 2,654,657 - +0.3 2,654,759 + +0.7 2,654,793 - -0.1 2,654,942 + -0.5 2,655,006 + -1.5 2,655,007 - -1.0 2,655,127 - LU632_RS13835 0.14 +1.2 2,655,174 + LU632_RS13835 0.18 +0.7 2,655,175 - LU632_RS13835 0.18 +0.8 2,655,229 - LU632_RS13835 0.22 -0.8 2,655,237 - LU632_RS13835 0.23 -0.2 2,655,349 + LU632_RS13835 0.32 -0.4 2,655,499 + LU632_RS13835 0.43 -0.1 2,655,500 - LU632_RS13835 0.43 +1.3 2,655,500 - LU632_RS13835 0.43 +0.9 2,655,507 - LU632_RS13835 0.44 +0.9 2,655,507 - LU632_RS13835 0.44 +0.3 2,655,534 - LU632_RS13835 0.46 +0.2 2,655,534 - LU632_RS13835 0.46 -0.4 2,655,662 - LU632_RS13835 0.56 -0.3 2,655,662 - LU632_RS13835 0.56 -0.5 2,655,962 - LU632_RS13835 0.79 +0.4 2,655,967 - LU632_RS13835 0.80 -1.7 2,655,967 - LU632_RS13835 0.80 -0.0 2,656,015 - LU632_RS13835 0.83 -0.6 2,656,230 + -0.3 2,656,259 + -0.3 2,656,388 + -1.0 2,656,433 + -0.4 2,656,433 + -0.2 2,656,471 + +0.0 2,656,471 + -0.6 2,656,508 - +0.4 2,656,518 + -0.4 2,656,702 + fliD LU632_RS13840 0.14 -0.7 2,656,786 + fliD LU632_RS13840 0.20 -1.9 2,656,790 + fliD LU632_RS13840 0.20 -0.8 2,656,791 - fliD LU632_RS13840 0.20 +0.4 2,656,888 + fliD LU632_RS13840 0.27 -0.1 2,656,889 - fliD LU632_RS13840 0.27 +0.7 2,657,005 + fliD LU632_RS13840 0.35 +0.4 2,657,006 - fliD LU632_RS13840 0.35 -0.1 2,657,006 - fliD LU632_RS13840 0.35 -0.3 2,657,031 - fliD LU632_RS13840 0.37 -1.4 2,657,218 + fliD LU632_RS13840 0.50 -1.2
Or see this region's nucleotide sequence