Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13610 and LU632_RS13615 are separated by 193 nucleotides LU632_RS13615 and LU632_RS13620 are separated by 37 nucleotides LU632_RS13620 and LU632_RS13625 are separated by 393 nucleotides
LU632_RS13610: LU632_RS13610 - glutamine amidotransferase, at 2,600,452 to 2,601,165
_RS13610
LU632_RS13615: LU632_RS13615 - molecular chaperone, at 2,601,359 to 2,601,919
_RS13615
LU632_RS13620: LU632_RS13620 - phosphatase, at 2,601,957 to 2,602,193
_RS13620
LU632_RS13625: LU632_RS13625 - hypothetical protein, at 2,602,587 to 2,602,844
_RS13625
Position (kb)
2601
2602 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2600.371 kb on + strand at 2600.536 kb on - strand, within LU632_RS13610 at 2600.694 kb on + strand, within LU632_RS13610 at 2600.763 kb on - strand, within LU632_RS13610 at 2600.847 kb on - strand, within LU632_RS13610 at 2600.854 kb on - strand, within LU632_RS13610 at 2601.039 kb on + strand, within LU632_RS13610 at 2601.084 kb on - strand, within LU632_RS13610 at 2601.103 kb on + strand at 2601.139 kb on - strand at 2601.139 kb on - strand at 2601.143 kb on - strand at 2601.167 kb on - strand at 2601.169 kb on + strand at 2601.175 kb on - strand at 2601.187 kb on - strand at 2601.250 kb on + strand at 2601.251 kb on - strand at 2601.265 kb on - strand at 2601.265 kb on - strand at 2601.265 kb on - strand at 2601.332 kb on + strand at 2601.332 kb on + strand at 2601.332 kb on + strand at 2601.333 kb on - strand at 2601.336 kb on + strand at 2601.336 kb on + strand at 2601.336 kb on + strand at 2601.336 kb on + strand at 2601.337 kb on - strand at 2601.337 kb on - strand at 2601.504 kb on + strand, within LU632_RS13615 at 2601.504 kb on + strand, within LU632_RS13615 at 2601.504 kb on + strand, within LU632_RS13615 at 2601.581 kb on + strand, within LU632_RS13615 at 2601.614 kb on - strand, within LU632_RS13615 at 2601.730 kb on - strand, within LU632_RS13615 at 2601.730 kb on - strand, within LU632_RS13615 at 2601.835 kb on + strand, within LU632_RS13615 at 2601.927 kb on + strand at 2602.035 kb on + strand, within LU632_RS13620 at 2602.067 kb on + strand, within LU632_RS13620 at 2602.182 kb on - strand at 2602.226 kb on - strand at 2602.234 kb on - strand at 2602.234 kb on - strand at 2602.269 kb on - strand at 2602.329 kb on - strand at 2602.340 kb on + strand at 2602.341 kb on - strand at 2602.358 kb on + strand at 2602.499 kb on + strand at 2602.536 kb on - strand at 2602.618 kb on - strand, within LU632_RS13625 at 2602.684 kb on - strand, within LU632_RS13625 at 2602.794 kb on - strand, within LU632_RS13625 at 2602.823 kb on + strand at 2602.823 kb on + strand at 2602.833 kb on + strand at 2602.833 kb on + strand at 2602.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,600,371 + -0.3 2,600,536 - LU632_RS13610 0.12 +1.7 2,600,694 + LU632_RS13610 0.34 -0.3 2,600,763 - LU632_RS13610 0.44 -1.3 2,600,847 - LU632_RS13610 0.55 -0.3 2,600,854 - LU632_RS13610 0.56 -0.5 2,601,039 + LU632_RS13610 0.82 -0.7 2,601,084 - LU632_RS13610 0.89 -0.7 2,601,103 + -0.6 2,601,139 - +0.1 2,601,139 - -0.1 2,601,143 - +0.4 2,601,167 - -0.4 2,601,169 + +0.1 2,601,175 - -0.1 2,601,187 - -1.8 2,601,250 + +0.4 2,601,251 - -0.7 2,601,265 - +0.0 2,601,265 - +0.7 2,601,265 - -0.7 2,601,332 + +0.6 2,601,332 + -0.7 2,601,332 + -0.6 2,601,333 - +0.6 2,601,336 + +0.0 2,601,336 + -2.6 2,601,336 + +0.0 2,601,336 + -0.2 2,601,337 - -0.1 2,601,337 - +0.1 2,601,504 + LU632_RS13615 0.26 -0.5 2,601,504 + LU632_RS13615 0.26 -0.9 2,601,504 + LU632_RS13615 0.26 +0.2 2,601,581 + LU632_RS13615 0.40 +0.3 2,601,614 - LU632_RS13615 0.45 -1.5 2,601,730 - LU632_RS13615 0.66 -0.1 2,601,730 - LU632_RS13615 0.66 -0.4 2,601,835 + LU632_RS13615 0.85 -0.0 2,601,927 + -1.1 2,602,035 + LU632_RS13620 0.33 +0.4 2,602,067 + LU632_RS13620 0.46 +0.2 2,602,182 - -1.3 2,602,226 - +2.2 2,602,234 - +0.1 2,602,234 - +1.2 2,602,269 - -0.3 2,602,329 - -0.9 2,602,340 + -0.4 2,602,341 - -0.0 2,602,358 + +0.9 2,602,499 + +0.9 2,602,536 - +0.6 2,602,618 - LU632_RS13625 0.12 -0.2 2,602,684 - LU632_RS13625 0.38 +0.8 2,602,794 - LU632_RS13625 0.80 +0.6 2,602,823 + -0.9 2,602,823 + +0.5 2,602,833 + -0.7 2,602,833 + +1.1 2,602,906 - +0.9
Or see this region's nucleotide sequence