Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pykF and LU632_RS11150 are separated by 23 nucleotides LU632_RS11150 and LU632_RS11155 are separated by 161 nucleotides LU632_RS11155 and LU632_RS11160 are separated by 9 nucleotides LU632_RS11160 and LU632_RS11165 are separated by 4 nucleotides LU632_RS11165 and LU632_RS11170 are separated by 148 nucleotides LU632_RS11170 and LU632_RS11175 are separated by 278 nucleotides
LU632_RS11145: pykF - pyruvate kinase PykF, at 2,133,674 to 2,135,086
pykF
LU632_RS11150: LU632_RS11150 - hypothetical protein, at 2,135,110 to 2,135,280
_RS11150
LU632_RS11155: LU632_RS11155 - tRNA-Val, at 2,135,442 to 2,135,518
_RS11155
LU632_RS11160: LU632_RS11160 - tRNA-Val, at 2,135,528 to 2,135,604
_RS11160
LU632_RS11165: LU632_RS11165 - tRNA-Val, at 2,135,609 to 2,135,685
_RS11165
LU632_RS11170: LU632_RS11170 - MATE family efflux transporter, at 2,135,834 to 2,137,207
_RS11170
LU632_RS11175: LU632_RS11175 - riboflavin synthase subunit alpha, at 2,137,486 to 2,138,139
_RS11175
Position (kb)
2135
2136
2137
2138 Strain fitness (log2 ratio)
-1
0
1
2 at 2134.942 kb on + strand, within pykF at 2135.006 kb on - strand at 2135.095 kb on - strand at 2135.101 kb on + strand at 2135.240 kb on - strand, within LU632_RS11150 at 2135.324 kb on + strand at 2135.374 kb on + strand at 2135.452 kb on + strand, within LU632_RS11155 at 2135.510 kb on + strand, within LU632_RS11155 at 2135.882 kb on - strand at 2135.890 kb on + strand at 2135.933 kb on - strand at 2135.997 kb on + strand, within LU632_RS11170 at 2136.025 kb on + strand, within LU632_RS11170 at 2136.037 kb on + strand, within LU632_RS11170 at 2136.050 kb on - strand, within LU632_RS11170 at 2136.178 kb on - strand, within LU632_RS11170 at 2136.381 kb on - strand, within LU632_RS11170 at 2136.506 kb on + strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170 at 2136.787 kb on - strand, within LU632_RS11170 at 2136.819 kb on + strand, within LU632_RS11170 at 2136.888 kb on + strand, within LU632_RS11170 at 2136.888 kb on + strand, within LU632_RS11170 at 2136.921 kb on - strand, within LU632_RS11170 at 2136.933 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.996 kb on - strand, within LU632_RS11170 at 2136.996 kb on - strand, within LU632_RS11170 at 2137.036 kb on + strand, within LU632_RS11170 at 2137.036 kb on + strand, within LU632_RS11170 at 2137.160 kb on + strand at 2137.162 kb on + strand at 2137.211 kb on + strand at 2137.212 kb on - strand at 2137.212 kb on - strand at 2137.212 kb on - strand at 2137.272 kb on - strand at 2137.272 kb on - strand at 2137.272 kb on - strand at 2137.343 kb on + strand at 2137.368 kb on - strand at 2137.368 kb on - strand at 2137.368 kb on - strand at 2137.372 kb on + strand at 2137.386 kb on + strand at 2137.387 kb on - strand at 2137.431 kb on + strand at 2138.137 kb on + strand at 2138.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,134,942 + pykF LU632_RS11145 0.90 +0.0 2,135,006 - -1.3 2,135,095 - +0.1 2,135,101 + -0.1 2,135,240 - LU632_RS11150 0.76 -0.4 2,135,324 + -1.2 2,135,374 + +0.1 2,135,452 + LU632_RS11155 0.13 +0.4 2,135,510 + LU632_RS11155 0.88 -0.8 2,135,882 - -1.3 2,135,890 + -0.1 2,135,933 - +0.3 2,135,997 + LU632_RS11170 0.12 -0.1 2,136,025 + LU632_RS11170 0.14 +0.1 2,136,037 + LU632_RS11170 0.15 +2.6 2,136,050 - LU632_RS11170 0.16 +0.8 2,136,178 - LU632_RS11170 0.25 -0.4 2,136,381 - LU632_RS11170 0.40 -0.8 2,136,506 + LU632_RS11170 0.49 +0.2 2,136,507 - LU632_RS11170 0.49 -1.3 2,136,507 - LU632_RS11170 0.49 +0.8 2,136,507 - LU632_RS11170 0.49 -0.1 2,136,507 - LU632_RS11170 0.49 +0.5 2,136,526 + LU632_RS11170 0.50 +0.0 2,136,526 + LU632_RS11170 0.50 +1.4 2,136,787 - LU632_RS11170 0.69 -0.4 2,136,819 + LU632_RS11170 0.72 +0.7 2,136,888 + LU632_RS11170 0.77 -0.6 2,136,888 + LU632_RS11170 0.77 +0.0 2,136,921 - LU632_RS11170 0.79 -0.2 2,136,933 + LU632_RS11170 0.80 -0.3 2,136,984 + LU632_RS11170 0.84 -0.8 2,136,984 + LU632_RS11170 0.84 -0.6 2,136,984 + LU632_RS11170 0.84 -0.6 2,136,985 - LU632_RS11170 0.84 +0.8 2,136,985 - LU632_RS11170 0.84 +0.3 2,136,985 - LU632_RS11170 0.84 +0.7 2,136,996 - LU632_RS11170 0.85 +0.3 2,136,996 - LU632_RS11170 0.85 +1.2 2,137,036 + LU632_RS11170 0.87 -0.1 2,137,036 + LU632_RS11170 0.87 +0.1 2,137,160 + +0.8 2,137,162 + +0.3 2,137,211 + -0.4 2,137,212 - -0.4 2,137,212 - -0.9 2,137,212 - +0.7 2,137,272 - -0.5 2,137,272 - -0.4 2,137,272 - -0.3 2,137,343 + +1.3 2,137,368 - -0.3 2,137,368 - -1.5 2,137,368 - -0.3 2,137,372 + +0.8 2,137,386 + -1.4 2,137,387 - +0.7 2,137,431 + +1.1 2,138,137 + +0.4 2,138,138 - -0.4
Or see this region's nucleotide sequence