Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10130 and LU632_RS10135 are separated by 99 nucleotides LU632_RS10135 and wrbA are separated by 20 nucleotides wrbA and LU632_RS10145 are separated by 495 nucleotides
LU632_RS10130: LU632_RS10130 - IS256 family transposase, at 1,937,658 to 1,938,866
_RS10130
LU632_RS10135: LU632_RS10135 - YccJ family protein, at 1,938,966 to 1,939,196
_RS10135
LU632_RS10140: wrbA - NAD(P)H:quinone oxidoreductase, at 1,939,217 to 1,939,816
wrbA
LU632_RS10145: LU632_RS10145 - isochorismatase family protein, at 1,940,312 to 1,940,996
_RS10145
Position (kb)
1939
1940 Strain fitness (log2 ratio)
-1
0
1 at 1938.864 kb on + strand at 1938.992 kb on + strand, within LU632_RS10135 at 1938.992 kb on + strand, within LU632_RS10135 at 1939.056 kb on - strand, within LU632_RS10135 at 1939.056 kb on - strand, within LU632_RS10135 at 1939.096 kb on + strand, within LU632_RS10135 at 1939.140 kb on - strand, within LU632_RS10135 at 1939.179 kb on - strand at 1939.251 kb on + strand at 1939.252 kb on - strand at 1939.252 kb on - strand at 1939.364 kb on - strand, within wrbA at 1939.364 kb on - strand, within wrbA at 1939.504 kb on - strand, within wrbA at 1939.608 kb on + strand, within wrbA at 1939.716 kb on - strand, within wrbA at 1939.837 kb on - strand at 1939.860 kb on - strand at 1939.860 kb on - strand at 1939.897 kb on - strand at 1939.955 kb on + strand at 1939.956 kb on - strand at 1939.956 kb on - strand at 1939.957 kb on + strand at 1939.957 kb on + strand at 1939.957 kb on + strand at 1939.973 kb on + strand at 1939.985 kb on - strand at 1939.985 kb on - strand at 1940.002 kb on + strand at 1940.002 kb on + strand at 1940.151 kb on - strand at 1940.180 kb on - strand at 1940.214 kb on + strand at 1940.299 kb on + strand at 1940.300 kb on - strand at 1940.303 kb on + strand at 1940.375 kb on - strand at 1940.473 kb on + strand, within LU632_RS10145 at 1940.474 kb on - strand, within LU632_RS10145 at 1940.540 kb on + strand, within LU632_RS10145 at 1940.540 kb on + strand, within LU632_RS10145 at 1940.591 kb on - strand, within LU632_RS10145 at 1940.591 kb on - strand, within LU632_RS10145 at 1940.622 kb on + strand, within LU632_RS10145 at 1940.622 kb on + strand, within LU632_RS10145 at 1940.622 kb on + strand, within LU632_RS10145 at 1940.623 kb on - strand, within LU632_RS10145 at 1940.623 kb on - strand, within LU632_RS10145 at 1940.625 kb on - strand, within LU632_RS10145 at 1940.625 kb on - strand, within LU632_RS10145 at 1940.775 kb on - strand, within LU632_RS10145 at 1940.775 kb on - strand, within LU632_RS10145
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,938,864 + +0.8 1,938,992 + LU632_RS10135 0.11 -1.1 1,938,992 + LU632_RS10135 0.11 -1.6 1,939,056 - LU632_RS10135 0.39 -0.1 1,939,056 - LU632_RS10135 0.39 +0.3 1,939,096 + LU632_RS10135 0.56 +0.4 1,939,140 - LU632_RS10135 0.75 +0.7 1,939,179 - -1.0 1,939,251 + -1.0 1,939,252 - +0.1 1,939,252 - -0.0 1,939,364 - wrbA LU632_RS10140 0.24 +0.5 1,939,364 - wrbA LU632_RS10140 0.24 -0.0 1,939,504 - wrbA LU632_RS10140 0.48 -1.1 1,939,608 + wrbA LU632_RS10140 0.65 -0.1 1,939,716 - wrbA LU632_RS10140 0.83 -0.9 1,939,837 - -0.1 1,939,860 - -0.3 1,939,860 - -0.1 1,939,897 - +0.1 1,939,955 + +0.5 1,939,956 - +0.9 1,939,956 - -0.9 1,939,957 + +0.5 1,939,957 + -1.0 1,939,957 + +0.0 1,939,973 + -0.6 1,939,985 - +0.5 1,939,985 - +0.4 1,940,002 + +0.5 1,940,002 + -0.1 1,940,151 - -0.4 1,940,180 - +0.3 1,940,214 + -0.4 1,940,299 + -0.3 1,940,300 - -0.7 1,940,303 + +0.7 1,940,375 - -0.8 1,940,473 + LU632_RS10145 0.24 -0.0 1,940,474 - LU632_RS10145 0.24 -0.1 1,940,540 + LU632_RS10145 0.33 +0.1 1,940,540 + LU632_RS10145 0.33 -0.3 1,940,591 - LU632_RS10145 0.41 +0.2 1,940,591 - LU632_RS10145 0.41 -0.1 1,940,622 + LU632_RS10145 0.45 +0.2 1,940,622 + LU632_RS10145 0.45 +0.2 1,940,622 + LU632_RS10145 0.45 -0.1 1,940,623 - LU632_RS10145 0.45 +0.4 1,940,623 - LU632_RS10145 0.45 -0.1 1,940,625 - LU632_RS10145 0.46 +0.1 1,940,625 - LU632_RS10145 0.46 +0.1 1,940,775 - LU632_RS10145 0.68 +0.7 1,940,775 - LU632_RS10145 0.68 -0.1
Or see this region's nucleotide sequence