Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10075 and LU632_RS10080 are separated by 63 nucleotides LU632_RS10080 and hspQ are separated by 12 nucleotides hspQ and rlmI are separated by 58 nucleotides rlmI and yccX are separated by 87 nucleotides yccX and LU632_RS10100 are separated by 42 nucleotides LU632_RS10100 and yccA are separated by 119 nucleotides
LU632_RS10075: LU632_RS10075 - IS1 family transposase, at 1,928,555 to 1,929,252
_RS10075
LU632_RS10080: LU632_RS10080 - CoA-binding protein, at 1,929,316 to 1,929,729
_RS10080
LU632_RS10085: hspQ - heat shock protein HspQ, at 1,929,742 to 1,930,058
hspQ
LU632_RS10090: rlmI - 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI, at 1,930,117 to 1,931,307
rlmI
LU632_RS10095: yccX - acylphosphatase, at 1,931,395 to 1,931,672
yccX
LU632_RS10100: LU632_RS10100 - TusE/DsrC/DsvC family sulfur relay protein, at 1,931,715 to 1,932,041
_RS10100
LU632_RS10105: yccA - FtsH protease modulator YccA, at 1,932,161 to 1,932,817
yccA
Position (kb) 1930
1931
1932 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1929.284 kb on - strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.339 kb on + strand at 1929.403 kb on + strand, within LU632_RS10080 at 1929.403 kb on + strand, within LU632_RS10080 at 1929.404 kb on - strand, within LU632_RS10080 at 1929.406 kb on - strand, within LU632_RS10080 at 1929.406 kb on - strand, within LU632_RS10080 at 1929.418 kb on + strand, within LU632_RS10080 at 1929.436 kb on + strand, within LU632_RS10080 at 1929.439 kb on - strand, within LU632_RS10080 at 1929.444 kb on - strand, within LU632_RS10080 at 1929.631 kb on - strand, within LU632_RS10080 at 1929.675 kb on + strand, within LU632_RS10080 at 1929.675 kb on + strand, within LU632_RS10080 at 1929.743 kb on + strand at 1929.766 kb on - strand at 1929.785 kb on + strand, within hspQ at 1929.785 kb on + strand, within hspQ at 1929.785 kb on + strand, within hspQ at 1929.859 kb on + strand, within hspQ at 1929.859 kb on + strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.982 kb on - strand, within hspQ at 1930.016 kb on + strand, within hspQ at 1930.016 kb on + strand, within hspQ at 1930.078 kb on - strand at 1930.078 kb on - strand at 1930.148 kb on + strand at 1930.148 kb on + strand at 1930.150 kb on + strand at 1930.150 kb on + strand at 1930.151 kb on - strand at 1930.151 kb on - strand at 1930.151 kb on - strand at 1930.341 kb on + strand, within rlmI at 1930.342 kb on - strand, within rlmI at 1930.345 kb on + strand, within rlmI at 1930.345 kb on + strand, within rlmI at 1930.345 kb on + strand, within rlmI at 1930.345 kb on + strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.346 kb on - strand, within rlmI at 1930.413 kb on + strand, within rlmI at 1930.414 kb on - strand, within rlmI at 1930.535 kb on - strand, within rlmI at 1930.557 kb on + strand, within rlmI at 1930.558 kb on - strand, within rlmI at 1930.568 kb on - strand, within rlmI at 1930.632 kb on + strand, within rlmI at 1930.639 kb on - strand, within rlmI at 1930.655 kb on + strand, within rlmI at 1930.656 kb on - strand, within rlmI at 1930.694 kb on + strand, within rlmI at 1930.694 kb on + strand, within rlmI at 1930.730 kb on - strand, within rlmI at 1930.962 kb on + strand, within rlmI at 1930.963 kb on - strand, within rlmI at 1931.007 kb on + strand, within rlmI at 1931.008 kb on - strand, within rlmI at 1931.062 kb on - strand, within rlmI at 1931.083 kb on - strand, within rlmI at 1931.166 kb on + strand, within rlmI at 1931.167 kb on - strand, within rlmI at 1931.212 kb on - strand at 1931.212 kb on - strand at 1931.307 kb on + strand at 1931.307 kb on + strand at 1931.314 kb on + strand at 1931.314 kb on + strand at 1931.320 kb on + strand at 1931.321 kb on - strand at 1931.321 kb on - strand at 1931.425 kb on + strand, within yccX at 1931.504 kb on + strand, within yccX at 1931.504 kb on + strand, within yccX at 1931.504 kb on + strand, within yccX at 1931.505 kb on - strand, within yccX at 1931.505 kb on - strand, within yccX at 1931.505 kb on - strand, within yccX at 1931.505 kb on - strand, within yccX at 1931.570 kb on + strand, within yccX at 1931.571 kb on - strand, within yccX at 1931.571 kb on - strand, within yccX at 1931.620 kb on + strand, within yccX at 1931.651 kb on - strand at 1931.662 kb on + strand at 1931.707 kb on - strand at 1931.707 kb on - strand at 1932.070 kb on - strand at 1932.213 kb on + strand at 1932.213 kb on + strand at 1932.213 kb on + strand at 1932.275 kb on - strand, within yccA at 1932.275 kb on - strand, within yccA at 1932.277 kb on + strand, within yccA
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,929,284 - +0.5 1,929,313 + +0.8 1,929,313 + +0.5 1,929,313 + +1.7 1,929,313 + -0.3 1,929,339 + -0.6 1,929,403 + LU632_RS10080 0.21 +0.3 1,929,403 + LU632_RS10080 0.21 +0.0 1,929,404 - LU632_RS10080 0.21 -0.3 1,929,406 - LU632_RS10080 0.22 +0.1 1,929,406 - LU632_RS10080 0.22 +0.3 1,929,418 + LU632_RS10080 0.25 +0.3 1,929,436 + LU632_RS10080 0.29 -1.5 1,929,439 - LU632_RS10080 0.30 +0.2 1,929,444 - LU632_RS10080 0.31 +0.8 1,929,631 - LU632_RS10080 0.76 +0.2 1,929,675 + LU632_RS10080 0.87 -0.2 1,929,675 + LU632_RS10080 0.87 +0.6 1,929,743 + +2.2 1,929,766 - -0.0 1,929,785 + hspQ LU632_RS10085 0.14 -0.4 1,929,785 + hspQ LU632_RS10085 0.14 +0.9 1,929,785 + hspQ LU632_RS10085 0.14 -0.0 1,929,859 + hspQ LU632_RS10085 0.37 +0.5 1,929,859 + hspQ LU632_RS10085 0.37 +0.5 1,929,864 - hspQ LU632_RS10085 0.38 -0.0 1,929,864 - hspQ LU632_RS10085 0.38 -0.5 1,929,864 - hspQ LU632_RS10085 0.38 +0.5 1,929,982 - hspQ LU632_RS10085 0.76 +0.1 1,930,016 + hspQ LU632_RS10085 0.86 -0.0 1,930,016 + hspQ LU632_RS10085 0.86 +0.7 1,930,078 - -0.5 1,930,078 - -0.7 1,930,148 + -0.4 1,930,148 + +1.9 1,930,150 + +0.4 1,930,150 + -0.5 1,930,151 - -1.4 1,930,151 - +0.6 1,930,151 - -0.8 1,930,341 + rlmI LU632_RS10090 0.19 +0.4 1,930,342 - rlmI LU632_RS10090 0.19 -0.3 1,930,345 + rlmI LU632_RS10090 0.19 -0.3 1,930,345 + rlmI LU632_RS10090 0.19 +0.3 1,930,345 + rlmI LU632_RS10090 0.19 +0.1 1,930,345 + rlmI LU632_RS10090 0.19 +0.4 1,930,346 - rlmI LU632_RS10090 0.19 +0.7 1,930,346 - rlmI LU632_RS10090 0.19 -0.3 1,930,346 - rlmI LU632_RS10090 0.19 -0.5 1,930,346 - rlmI LU632_RS10090 0.19 -0.2 1,930,346 - rlmI LU632_RS10090 0.19 -0.1 1,930,346 - rlmI LU632_RS10090 0.19 -0.7 1,930,413 + rlmI LU632_RS10090 0.25 -0.2 1,930,414 - rlmI LU632_RS10090 0.25 -1.1 1,930,535 - rlmI LU632_RS10090 0.35 -1.9 1,930,557 + rlmI LU632_RS10090 0.37 -0.2 1,930,558 - rlmI LU632_RS10090 0.37 -0.5 1,930,568 - rlmI LU632_RS10090 0.38 -0.3 1,930,632 + rlmI LU632_RS10090 0.43 +0.3 1,930,639 - rlmI LU632_RS10090 0.44 -0.4 1,930,655 + rlmI LU632_RS10090 0.45 +0.2 1,930,656 - rlmI LU632_RS10090 0.45 +1.2 1,930,694 + rlmI LU632_RS10090 0.48 +0.6 1,930,694 + rlmI LU632_RS10090 0.48 -0.1 1,930,730 - rlmI LU632_RS10090 0.51 +0.3 1,930,962 + rlmI LU632_RS10090 0.71 -0.1 1,930,963 - rlmI LU632_RS10090 0.71 -0.1 1,931,007 + rlmI LU632_RS10090 0.75 -0.8 1,931,008 - rlmI LU632_RS10090 0.75 -0.3 1,931,062 - rlmI LU632_RS10090 0.79 -0.1 1,931,083 - rlmI LU632_RS10090 0.81 -0.8 1,931,166 + rlmI LU632_RS10090 0.88 -0.4 1,931,167 - rlmI LU632_RS10090 0.88 +0.2 1,931,212 - +1.5 1,931,212 - +0.2 1,931,307 + -0.9 1,931,307 + -0.1 1,931,314 + +0.9 1,931,314 + -0.3 1,931,320 + -0.3 1,931,321 - -0.4 1,931,321 - +0.0 1,931,425 + yccX LU632_RS10095 0.11 +0.7 1,931,504 + yccX LU632_RS10095 0.39 +0.5 1,931,504 + yccX LU632_RS10095 0.39 -0.3 1,931,504 + yccX LU632_RS10095 0.39 -0.1 1,931,505 - yccX LU632_RS10095 0.40 -0.5 1,931,505 - yccX LU632_RS10095 0.40 -0.7 1,931,505 - yccX LU632_RS10095 0.40 -0.5 1,931,505 - yccX LU632_RS10095 0.40 +1.9 1,931,570 + yccX LU632_RS10095 0.63 -0.4 1,931,571 - yccX LU632_RS10095 0.63 +1.7 1,931,571 - yccX LU632_RS10095 0.63 +0.3 1,931,620 + yccX LU632_RS10095 0.81 -0.1 1,931,651 - +0.0 1,931,662 + +0.7 1,931,707 - +0.1 1,931,707 - +0.5 1,932,070 - +0.2 1,932,213 + +0.9 1,932,213 + +0.4 1,932,213 + +0.5 1,932,275 - yccA LU632_RS10105 0.17 -2.2 1,932,275 - yccA LU632_RS10105 0.17 -0.1 1,932,277 + yccA LU632_RS10105 0.18 -0.4
Or see this region's nucleotide sequence