Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10065 and LU632_RS10070 are separated by 36 nucleotides LU632_RS10070 and LU632_RS10075 are separated by 196 nucleotides LU632_RS10075 and LU632_RS10080 are separated by 63 nucleotides LU632_RS10080 and hspQ are separated by 12 nucleotides hspQ and rlmI are separated by 58 nucleotides
LU632_RS10065: LU632_RS10065 - IS256 family transposase, at 1,926,925 to 1,928,133
_RS10065
LU632_RS10070: LU632_RS10070 - DUF2057 family protein, at 1,928,170 to 1,928,358
_RS10070
LU632_RS10075: LU632_RS10075 - IS1 family transposase, at 1,928,555 to 1,929,252
_RS10075
LU632_RS10080: LU632_RS10080 - CoA-binding protein, at 1,929,316 to 1,929,729
_RS10080
LU632_RS10085: hspQ - heat shock protein HspQ, at 1,929,742 to 1,930,058
hspQ
LU632_RS10090: rlmI - 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI, at 1,930,117 to 1,931,307
rlmI
Position (kb) 1928
1929
1930 Strain fitness (log2 ratio) -1
0
1
2 at 1928.146 kb on + strand at 1928.156 kb on + strand at 1928.224 kb on + strand, within LU632_RS10070 at 1928.225 kb on - strand, within LU632_RS10070 at 1928.228 kb on - strand, within LU632_RS10070 at 1928.263 kb on + strand, within LU632_RS10070 at 1928.268 kb on + strand, within LU632_RS10070 at 1928.329 kb on - strand, within LU632_RS10070 at 1928.555 kb on - strand at 1928.555 kb on - strand at 1929.284 kb on - strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.313 kb on + strand at 1929.339 kb on + strand at 1929.403 kb on + strand, within LU632_RS10080 at 1929.403 kb on + strand, within LU632_RS10080 at 1929.404 kb on - strand, within LU632_RS10080 at 1929.406 kb on - strand, within LU632_RS10080 at 1929.406 kb on - strand, within LU632_RS10080 at 1929.418 kb on + strand, within LU632_RS10080 at 1929.436 kb on + strand, within LU632_RS10080 at 1929.439 kb on - strand, within LU632_RS10080 at 1929.444 kb on - strand, within LU632_RS10080 at 1929.631 kb on - strand, within LU632_RS10080 at 1929.675 kb on + strand, within LU632_RS10080 at 1929.675 kb on + strand, within LU632_RS10080 at 1929.743 kb on + strand at 1929.766 kb on - strand at 1929.785 kb on + strand, within hspQ at 1929.785 kb on + strand, within hspQ at 1929.785 kb on + strand, within hspQ at 1929.859 kb on + strand, within hspQ at 1929.859 kb on + strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.864 kb on - strand, within hspQ at 1929.982 kb on - strand, within hspQ at 1930.016 kb on + strand, within hspQ at 1930.016 kb on + strand, within hspQ at 1930.078 kb on - strand at 1930.078 kb on - strand at 1930.148 kb on + strand at 1930.148 kb on + strand at 1930.150 kb on + strand at 1930.150 kb on + strand at 1930.151 kb on - strand at 1930.151 kb on - strand at 1930.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,928,146 + -0.4 1,928,156 + +0.3 1,928,224 + LU632_RS10070 0.29 -0.1 1,928,225 - LU632_RS10070 0.29 -0.5 1,928,228 - LU632_RS10070 0.31 -0.6 1,928,263 + LU632_RS10070 0.49 +0.3 1,928,268 + LU632_RS10070 0.52 -0.1 1,928,329 - LU632_RS10070 0.84 -0.1 1,928,555 - +0.3 1,928,555 - +0.0 1,929,284 - +0.5 1,929,313 + +0.8 1,929,313 + +0.5 1,929,313 + +1.7 1,929,313 + -0.3 1,929,339 + -0.6 1,929,403 + LU632_RS10080 0.21 +0.3 1,929,403 + LU632_RS10080 0.21 +0.0 1,929,404 - LU632_RS10080 0.21 -0.3 1,929,406 - LU632_RS10080 0.22 +0.1 1,929,406 - LU632_RS10080 0.22 +0.3 1,929,418 + LU632_RS10080 0.25 +0.3 1,929,436 + LU632_RS10080 0.29 -1.5 1,929,439 - LU632_RS10080 0.30 +0.2 1,929,444 - LU632_RS10080 0.31 +0.8 1,929,631 - LU632_RS10080 0.76 +0.2 1,929,675 + LU632_RS10080 0.87 -0.2 1,929,675 + LU632_RS10080 0.87 +0.6 1,929,743 + +2.2 1,929,766 - -0.0 1,929,785 + hspQ LU632_RS10085 0.14 -0.4 1,929,785 + hspQ LU632_RS10085 0.14 +0.9 1,929,785 + hspQ LU632_RS10085 0.14 -0.0 1,929,859 + hspQ LU632_RS10085 0.37 +0.5 1,929,859 + hspQ LU632_RS10085 0.37 +0.5 1,929,864 - hspQ LU632_RS10085 0.38 -0.0 1,929,864 - hspQ LU632_RS10085 0.38 -0.5 1,929,864 - hspQ LU632_RS10085 0.38 +0.5 1,929,982 - hspQ LU632_RS10085 0.76 +0.1 1,930,016 + hspQ LU632_RS10085 0.86 -0.0 1,930,016 + hspQ LU632_RS10085 0.86 +0.7 1,930,078 - -0.5 1,930,078 - -0.7 1,930,148 + -0.4 1,930,148 + +1.9 1,930,150 + +0.4 1,930,150 + -0.5 1,930,151 - -1.4 1,930,151 - +0.6 1,930,151 - -0.8
Or see this region's nucleotide sequence