Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqiB and pqiC overlap by 1 nucleotides pqiC and rmf are separated by 398 nucleotides rmf and fabA are separated by 101 nucleotides
LU632_RS09980: pqiB - intermembrane transport protein PqiB, at 1,909,049 to 1,910,689
pqiB
LU632_RS09985: pqiC - membrane integrity-associated transporter subunit PqiC, at 1,910,689 to 1,911,315
pqiC
LU632_RS09990: rmf - ribosome modulation factor, at 1,911,714 to 1,911,881
rmf
LU632_RS09995: fabA - bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase, at 1,911,983 to 1,912,501
fabA
Position (kb) 1910
1911
1912 Strain fitness (log2 ratio) -1
0
1
2 at 1909.860 kb on + strand, within pqiB at 1909.860 kb on + strand, within pqiB at 1909.861 kb on - strand, within pqiB at 1909.876 kb on + strand, within pqiB at 1909.895 kb on + strand, within pqiB at 1909.895 kb on + strand, within pqiB at 1909.990 kb on + strand, within pqiB at 1909.991 kb on - strand, within pqiB at 1909.991 kb on - strand, within pqiB at 1909.991 kb on - strand, within pqiB at 1910.143 kb on + strand, within pqiB at 1910.144 kb on - strand, within pqiB at 1910.199 kb on - strand, within pqiB at 1910.199 kb on - strand, within pqiB at 1910.330 kb on - strand, within pqiB at 1910.336 kb on - strand, within pqiB at 1910.338 kb on + strand, within pqiB at 1910.350 kb on - strand, within pqiB at 1910.469 kb on + strand, within pqiB at 1910.506 kb on + strand, within pqiB at 1910.543 kb on + strand at 1910.616 kb on + strand at 1910.623 kb on + strand at 1910.687 kb on + strand at 1910.688 kb on - strand at 1910.831 kb on + strand, within pqiC at 1910.838 kb on + strand, within pqiC at 1910.839 kb on - strand, within pqiC at 1910.839 kb on - strand, within pqiC at 1910.895 kb on + strand, within pqiC at 1910.895 kb on + strand, within pqiC at 1910.900 kb on - strand, within pqiC at 1910.916 kb on + strand, within pqiC at 1910.967 kb on + strand, within pqiC at 1911.126 kb on + strand, within pqiC at 1911.127 kb on - strand, within pqiC at 1911.362 kb on - strand at 1911.365 kb on + strand at 1911.366 kb on - strand at 1911.366 kb on - strand at 1911.650 kb on + strand at 1911.651 kb on - strand at 1911.909 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,909,860 + pqiB LU632_RS09980 0.49 +0.6 1,909,860 + pqiB LU632_RS09980 0.49 +0.4 1,909,861 - pqiB LU632_RS09980 0.49 +0.3 1,909,876 + pqiB LU632_RS09980 0.50 -0.5 1,909,895 + pqiB LU632_RS09980 0.52 -0.1 1,909,895 + pqiB LU632_RS09980 0.52 +0.0 1,909,990 + pqiB LU632_RS09980 0.57 +1.5 1,909,991 - pqiB LU632_RS09980 0.57 -1.6 1,909,991 - pqiB LU632_RS09980 0.57 +1.9 1,909,991 - pqiB LU632_RS09980 0.57 -0.1 1,910,143 + pqiB LU632_RS09980 0.67 -0.5 1,910,144 - pqiB LU632_RS09980 0.67 -1.7 1,910,199 - pqiB LU632_RS09980 0.70 -0.6 1,910,199 - pqiB LU632_RS09980 0.70 -0.3 1,910,330 - pqiB LU632_RS09980 0.78 -0.5 1,910,336 - pqiB LU632_RS09980 0.78 +0.7 1,910,338 + pqiB LU632_RS09980 0.79 +1.3 1,910,350 - pqiB LU632_RS09980 0.79 +0.0 1,910,469 + pqiB LU632_RS09980 0.87 -0.4 1,910,506 + pqiB LU632_RS09980 0.89 +0.1 1,910,543 + -0.9 1,910,616 + +0.4 1,910,623 + +1.7 1,910,687 + -0.7 1,910,688 - +0.5 1,910,831 + pqiC LU632_RS09985 0.23 -0.9 1,910,838 + pqiC LU632_RS09985 0.24 -0.9 1,910,839 - pqiC LU632_RS09985 0.24 -0.1 1,910,839 - pqiC LU632_RS09985 0.24 +0.5 1,910,895 + pqiC LU632_RS09985 0.33 -0.5 1,910,895 + pqiC LU632_RS09985 0.33 -0.9 1,910,900 - pqiC LU632_RS09985 0.34 +0.5 1,910,916 + pqiC LU632_RS09985 0.36 -0.2 1,910,967 + pqiC LU632_RS09985 0.44 -0.1 1,911,126 + pqiC LU632_RS09985 0.70 -0.1 1,911,127 - pqiC LU632_RS09985 0.70 -1.0 1,911,362 - +0.3 1,911,365 + -0.5 1,911,366 - +1.6 1,911,366 - -0.8 1,911,650 + -0.2 1,911,651 - +0.9 1,911,909 - +1.3
Or see this region's nucleotide sequence