Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trxB and LU632_RS09735 are separated by 93 nucleotides LU632_RS09735 and lrp are separated by 117 nucleotides lrp and LU632_RS09745 are separated by 118 nucleotides
LU632_RS09730: trxB - thioredoxin-disulfide reductase, at 1,845,795 to 1,846,763
trxB
LU632_RS09735: LU632_RS09735 - hypothetical protein, at 1,846,857 to 1,847,174
_RS09735
LU632_RS09740: lrp - leucine-responsive transcriptional regulator Lrp, at 1,847,292 to 1,847,786
lrp
LU632_RS09745: LU632_RS09745 - DNA translocase FtsK 4TM domain-containing protein, at 1,847,905 to 1,851,234
_RS09745
Position (kb)
1846
1847
1848 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1845.929 kb on + strand, within trxB at 1845.974 kb on + strand, within trxB at 1845.974 kb on + strand, within trxB at 1845.975 kb on - strand, within trxB at 1845.976 kb on + strand, within trxB at 1845.977 kb on - strand, within trxB at 1846.058 kb on + strand, within trxB at 1846.058 kb on + strand, within trxB at 1846.077 kb on - strand, within trxB at 1846.454 kb on - strand, within trxB at 1846.693 kb on + strand at 1846.696 kb on - strand at 1846.696 kb on - strand at 1846.900 kb on + strand, within LU632_RS09735 at 1846.954 kb on + strand, within LU632_RS09735 at 1846.955 kb on - strand, within LU632_RS09735 at 1846.986 kb on + strand, within LU632_RS09735 at 1846.986 kb on + strand, within LU632_RS09735 at 1846.999 kb on - strand, within LU632_RS09735 at 1847.006 kb on + strand, within LU632_RS09735 at 1847.054 kb on - strand, within LU632_RS09735 at 1847.085 kb on - strand, within LU632_RS09735 at 1847.087 kb on + strand, within LU632_RS09735 at 1847.087 kb on + strand, within LU632_RS09735 at 1847.088 kb on - strand, within LU632_RS09735 at 1847.090 kb on - strand, within LU632_RS09735 at 1847.119 kb on + strand, within LU632_RS09735 at 1847.122 kb on - strand, within LU632_RS09735 at 1847.303 kb on + strand at 1847.304 kb on - strand at 1847.463 kb on - strand, within lrp at 1847.475 kb on - strand, within lrp at 1847.497 kb on - strand, within lrp at 1847.519 kb on - strand, within lrp at 1847.519 kb on - strand, within lrp at 1847.726 kb on + strand, within lrp at 1847.727 kb on - strand, within lrp at 1847.727 kb on - strand, within lrp at 1847.730 kb on + strand, within lrp at 1847.730 kb on + strand, within lrp at 1847.774 kb on + strand at 1847.920 kb on - strand at 1847.988 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,845,929 + trxB LU632_RS09730 0.14 +0.9 1,845,974 + trxB LU632_RS09730 0.18 -1.1 1,845,974 + trxB LU632_RS09730 0.18 -0.7 1,845,975 - trxB LU632_RS09730 0.19 -2.6 1,845,976 + trxB LU632_RS09730 0.19 +0.9 1,845,977 - trxB LU632_RS09730 0.19 +2.5 1,846,058 + trxB LU632_RS09730 0.27 -2.1 1,846,058 + trxB LU632_RS09730 0.27 -0.7 1,846,077 - trxB LU632_RS09730 0.29 -1.1 1,846,454 - trxB LU632_RS09730 0.68 -0.3 1,846,693 + -0.4 1,846,696 - +0.9 1,846,696 - -1.8 1,846,900 + LU632_RS09735 0.14 +1.6 1,846,954 + LU632_RS09735 0.31 -0.9 1,846,955 - LU632_RS09735 0.31 -0.5 1,846,986 + LU632_RS09735 0.41 +0.2 1,846,986 + LU632_RS09735 0.41 -2.7 1,846,999 - LU632_RS09735 0.45 -0.1 1,847,006 + LU632_RS09735 0.47 -0.0 1,847,054 - LU632_RS09735 0.62 -1.7 1,847,085 - LU632_RS09735 0.72 -2.5 1,847,087 + LU632_RS09735 0.72 -0.5 1,847,087 + LU632_RS09735 0.72 -1.0 1,847,088 - LU632_RS09735 0.73 -1.7 1,847,090 - LU632_RS09735 0.73 -2.1 1,847,119 + LU632_RS09735 0.82 -2.3 1,847,122 - LU632_RS09735 0.83 -2.8 1,847,303 + -1.9 1,847,304 - -3.7 1,847,463 - lrp LU632_RS09740 0.35 -1.7 1,847,475 - lrp LU632_RS09740 0.37 -3.3 1,847,497 - lrp LU632_RS09740 0.41 -3.6 1,847,519 - lrp LU632_RS09740 0.46 -3.0 1,847,519 - lrp LU632_RS09740 0.46 -3.9 1,847,726 + lrp LU632_RS09740 0.88 -3.2 1,847,727 - lrp LU632_RS09740 0.88 -2.8 1,847,727 - lrp LU632_RS09740 0.88 -5.3 1,847,730 + lrp LU632_RS09740 0.88 -0.1 1,847,730 + lrp LU632_RS09740 0.88 -2.5 1,847,774 + -0.9 1,847,920 - -1.1 1,847,988 + -0.7
Or see this region's nucleotide sequence