Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and modB overlap by 7 nucleotides modB and modC overlap by 7 nucleotides modC and tnpA are separated by 66 nucleotides tnpA and LU632_RS09000 are separated by 23 nucleotides
LU632_RS08980: modA - molybdate ABC transporter substrate-binding protein, at 1,693,171 to 1,693,944
modA
LU632_RS08985: modB - molybdate ABC transporter permease subunit, at 1,693,938 to 1,694,633
modB
LU632_RS08990: modC - molybdenum ABC transporter ATP-binding protein ModC, at 1,694,627 to 1,695,685
modC
LU632_RS08995: tnpA - IS200/IS605 family transposase, at 1,695,752 to 1,696,168
tnpA
LU632_RS09000: LU632_RS09000 - transposase, at 1,696,192 to 1,697,400
_RS09000
Position (kb) 1694
1695
1696 Strain fitness (log2 ratio) -1
0
1 at 1693.747 kb on + strand, within modA at 1693.812 kb on + strand, within modA at 1693.826 kb on - strand, within modA at 1693.886 kb on + strand at 1693.886 kb on + strand at 1694.033 kb on - strand, within modB at 1694.128 kb on - strand, within modB at 1694.165 kb on - strand, within modB at 1694.178 kb on + strand, within modB at 1694.178 kb on + strand, within modB at 1694.179 kb on - strand, within modB at 1694.179 kb on - strand, within modB at 1694.181 kb on - strand, within modB at 1694.441 kb on + strand, within modB at 1694.442 kb on - strand, within modB at 1694.493 kb on + strand, within modB at 1694.493 kb on + strand, within modB at 1694.493 kb on + strand, within modB at 1694.493 kb on + strand, within modB at 1694.493 kb on + strand, within modB at 1694.494 kb on - strand, within modB at 1694.494 kb on - strand, within modB at 1694.494 kb on - strand, within modB at 1694.494 kb on - strand, within modB at 1694.494 kb on - strand, within modB at 1694.495 kb on + strand, within modB at 1694.495 kb on + strand, within modB at 1694.495 kb on + strand, within modB at 1694.632 kb on - strand at 1694.632 kb on - strand at 1694.632 kb on - strand at 1694.671 kb on + strand at 1694.746 kb on - strand, within modC at 1694.750 kb on - strand, within modC at 1694.922 kb on - strand, within modC at 1695.111 kb on + strand, within modC at 1695.115 kb on + strand, within modC at 1695.116 kb on - strand, within modC at 1695.116 kb on - strand, within modC at 1695.127 kb on - strand, within modC at 1695.139 kb on - strand, within modC at 1695.176 kb on + strand, within modC at 1695.176 kb on + strand, within modC at 1695.177 kb on - strand, within modC at 1695.223 kb on - strand, within modC at 1695.223 kb on - strand, within modC at 1695.223 kb on - strand, within modC at 1695.223 kb on - strand, within modC at 1695.228 kb on + strand, within modC at 1695.362 kb on + strand, within modC at 1695.397 kb on + strand, within modC at 1695.397 kb on + strand, within modC at 1695.397 kb on + strand, within modC at 1695.398 kb on - strand, within modC at 1695.511 kb on + strand, within modC at 1695.555 kb on + strand, within modC at 1695.579 kb on + strand, within modC at 1695.602 kb on - strand at 1695.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,693,747 + modA LU632_RS08980 0.74 -0.3 1,693,812 + modA LU632_RS08980 0.83 -0.4 1,693,826 - modA LU632_RS08980 0.85 -0.1 1,693,886 + -0.1 1,693,886 + +0.1 1,694,033 - modB LU632_RS08985 0.14 -0.0 1,694,128 - modB LU632_RS08985 0.27 -0.5 1,694,165 - modB LU632_RS08985 0.33 -0.3 1,694,178 + modB LU632_RS08985 0.34 -0.8 1,694,178 + modB LU632_RS08985 0.34 +0.0 1,694,179 - modB LU632_RS08985 0.35 +0.9 1,694,179 - modB LU632_RS08985 0.35 -0.6 1,694,181 - modB LU632_RS08985 0.35 -0.1 1,694,441 + modB LU632_RS08985 0.72 +1.5 1,694,442 - modB LU632_RS08985 0.72 -0.2 1,694,493 + modB LU632_RS08985 0.80 -0.3 1,694,493 + modB LU632_RS08985 0.80 -0.7 1,694,493 + modB LU632_RS08985 0.80 +0.1 1,694,493 + modB LU632_RS08985 0.80 +0.0 1,694,493 + modB LU632_RS08985 0.80 -0.6 1,694,494 - modB LU632_RS08985 0.80 -0.0 1,694,494 - modB LU632_RS08985 0.80 -0.4 1,694,494 - modB LU632_RS08985 0.80 -0.1 1,694,494 - modB LU632_RS08985 0.80 -0.6 1,694,494 - modB LU632_RS08985 0.80 +0.2 1,694,495 + modB LU632_RS08985 0.80 -0.9 1,694,495 + modB LU632_RS08985 0.80 -1.1 1,694,495 + modB LU632_RS08985 0.80 +0.1 1,694,632 - +0.5 1,694,632 - +0.3 1,694,632 - -0.7 1,694,671 + -0.0 1,694,746 - modC LU632_RS08990 0.11 -1.3 1,694,750 - modC LU632_RS08990 0.12 +0.1 1,694,922 - modC LU632_RS08990 0.28 +0.7 1,695,111 + modC LU632_RS08990 0.46 +0.0 1,695,115 + modC LU632_RS08990 0.46 -0.5 1,695,116 - modC LU632_RS08990 0.46 -0.4 1,695,116 - modC LU632_RS08990 0.46 +0.5 1,695,127 - modC LU632_RS08990 0.47 +0.0 1,695,139 - modC LU632_RS08990 0.48 +0.6 1,695,176 + modC LU632_RS08990 0.52 -1.2 1,695,176 + modC LU632_RS08990 0.52 -0.6 1,695,177 - modC LU632_RS08990 0.52 -0.5 1,695,223 - modC LU632_RS08990 0.56 -0.6 1,695,223 - modC LU632_RS08990 0.56 -0.1 1,695,223 - modC LU632_RS08990 0.56 -0.5 1,695,223 - modC LU632_RS08990 0.56 -0.5 1,695,228 + modC LU632_RS08990 0.57 +0.9 1,695,362 + modC LU632_RS08990 0.69 +0.2 1,695,397 + modC LU632_RS08990 0.73 -0.3 1,695,397 + modC LU632_RS08990 0.73 -1.1 1,695,397 + modC LU632_RS08990 0.73 -1.4 1,695,398 - modC LU632_RS08990 0.73 -0.7 1,695,511 + modC LU632_RS08990 0.83 +0.5 1,695,555 + modC LU632_RS08990 0.88 -0.2 1,695,579 + modC LU632_RS08990 0.90 -0.0 1,695,602 - +0.0 1,695,662 - -0.2
Or see this region's nucleotide sequence