Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS08725 and LU632_RS08730 are separated by 57 nucleotides LU632_RS08730 and LU632_RS08735 are separated by 622 nucleotides LU632_RS08735 and udk are separated by 246 nucleotides udk and dcd are separated by 106 nucleotides
LU632_RS08725: LU632_RS08725 - PAS domain S-box protein, at 1,639,550 to 1,639,744
_RS08725
LU632_RS08730: LU632_RS08730 - hypothetical protein, at 1,639,802 to 1,640,107
_RS08730
LU632_RS08735: LU632_RS08735 - phosphatase PAP2 family protein, at 1,640,730 to 1,641,326
_RS08735
LU632_RS08740: udk - uridine kinase, at 1,641,573 to 1,642,214
udk
LU632_RS08745: dcd - dCTP deaminase, at 1,642,321 to 1,642,902
dcd
Position (kb)
1640
1641
1642 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1639.747 kb on + strand at 1639.780 kb on + strand at 1639.818 kb on - strand at 1639.967 kb on + strand, within LU632_RS08730 at 1639.987 kb on - strand, within LU632_RS08730 at 1639.991 kb on + strand, within LU632_RS08730 at 1639.991 kb on + strand, within LU632_RS08730 at 1639.991 kb on + strand, within LU632_RS08730 at 1639.991 kb on + strand, within LU632_RS08730 at 1639.991 kb on + strand, within LU632_RS08730 at 1639.992 kb on - strand, within LU632_RS08730 at 1639.992 kb on - strand, within LU632_RS08730 at 1639.995 kb on - strand, within LU632_RS08730 at 1639.995 kb on - strand, within LU632_RS08730 at 1640.071 kb on + strand, within LU632_RS08730 at 1640.109 kb on - strand at 1640.119 kb on - strand at 1640.149 kb on + strand at 1640.149 kb on + strand at 1640.149 kb on + strand at 1640.150 kb on - strand at 1640.334 kb on - strand at 1640.335 kb on - strand at 1640.357 kb on + strand at 1640.486 kb on - strand at 1640.520 kb on + strand at 1640.527 kb on + strand at 1640.527 kb on + strand at 1640.528 kb on - strand at 1640.528 kb on - strand at 1640.528 kb on - strand at 1640.530 kb on + strand at 1640.627 kb on - strand at 1640.644 kb on - strand at 1640.682 kb on + strand at 1640.683 kb on - strand at 1640.706 kb on - strand at 1640.715 kb on + strand at 1640.718 kb on + strand at 1640.718 kb on + strand at 1640.719 kb on - strand at 1640.719 kb on - strand at 1640.763 kb on + strand at 1640.764 kb on - strand at 1640.946 kb on - strand, within LU632_RS08735 at 1640.980 kb on - strand, within LU632_RS08735 at 1640.998 kb on + strand, within LU632_RS08735 at 1640.999 kb on - strand, within LU632_RS08735 at 1641.043 kb on + strand, within LU632_RS08735 at 1641.043 kb on + strand, within LU632_RS08735 at 1641.043 kb on + strand, within LU632_RS08735 at 1641.044 kb on - strand, within LU632_RS08735 at 1641.044 kb on - strand, within LU632_RS08735 at 1641.048 kb on + strand, within LU632_RS08735 at 1641.048 kb on + strand, within LU632_RS08735 at 1641.156 kb on - strand, within LU632_RS08735 at 1641.190 kb on - strand, within LU632_RS08735 at 1641.317 kb on + strand at 1641.318 kb on - strand at 1641.318 kb on - strand at 1641.351 kb on - strand at 1641.398 kb on + strand at 1641.398 kb on + strand at 1641.399 kb on - strand at 1641.426 kb on + strand at 1641.447 kb on + strand at 1641.447 kb on + strand at 1641.448 kb on - strand at 1641.483 kb on + strand at 1641.572 kb on + strand at 1641.581 kb on + strand at 1641.581 kb on + strand at 1641.604 kb on + strand at 1641.604 kb on + strand at 1641.608 kb on + strand at 1641.608 kb on + strand at 1641.717 kb on + strand, within udk at 1641.720 kb on + strand, within udk at 1641.764 kb on + strand, within udk at 1641.855 kb on + strand, within udk at 1641.855 kb on + strand, within udk at 1641.855 kb on + strand, within udk at 1641.855 kb on + strand, within udk at 1642.019 kb on + strand, within udk at 1642.121 kb on + strand, within udk at 1642.121 kb on + strand, within udk at 1642.128 kb on + strand, within udk at 1642.128 kb on + strand, within udk at 1642.139 kb on + strand, within udk at 1642.139 kb on + strand, within udk at 1642.236 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,639,747 + -1.0 1,639,780 + +0.2 1,639,818 - -0.1 1,639,967 + LU632_RS08730 0.54 +0.5 1,639,987 - LU632_RS08730 0.60 +1.1 1,639,991 + LU632_RS08730 0.62 +0.1 1,639,991 + LU632_RS08730 0.62 +0.2 1,639,991 + LU632_RS08730 0.62 +1.9 1,639,991 + LU632_RS08730 0.62 +0.3 1,639,991 + LU632_RS08730 0.62 +0.4 1,639,992 - LU632_RS08730 0.62 -0.3 1,639,992 - LU632_RS08730 0.62 -0.1 1,639,995 - LU632_RS08730 0.63 -0.1 1,639,995 - LU632_RS08730 0.63 -0.0 1,640,071 + LU632_RS08730 0.88 -1.1 1,640,109 - +0.0 1,640,119 - +0.3 1,640,149 + +1.0 1,640,149 + +0.3 1,640,149 + -0.5 1,640,150 - +0.0 1,640,334 - -0.1 1,640,335 - +0.4 1,640,357 + -0.3 1,640,486 - +0.9 1,640,520 + -0.1 1,640,527 + +0.3 1,640,527 + -0.2 1,640,528 - +0.7 1,640,528 - +0.0 1,640,528 - -0.2 1,640,530 + -0.2 1,640,627 - -0.1 1,640,644 - -0.4 1,640,682 + -0.0 1,640,683 - -0.4 1,640,706 - -0.4 1,640,715 + -0.4 1,640,718 + -0.1 1,640,718 + -0.1 1,640,719 - -1.8 1,640,719 - -0.4 1,640,763 + -0.4 1,640,764 - +0.3 1,640,946 - LU632_RS08735 0.36 -0.6 1,640,980 - LU632_RS08735 0.42 -0.3 1,640,998 + LU632_RS08735 0.45 -0.7 1,640,999 - LU632_RS08735 0.45 -0.7 1,641,043 + LU632_RS08735 0.52 -1.1 1,641,043 + LU632_RS08735 0.52 +0.3 1,641,043 + LU632_RS08735 0.52 -0.3 1,641,044 - LU632_RS08735 0.53 +0.6 1,641,044 - LU632_RS08735 0.53 -0.8 1,641,048 + LU632_RS08735 0.53 +0.6 1,641,048 + LU632_RS08735 0.53 +0.8 1,641,156 - LU632_RS08735 0.71 +0.2 1,641,190 - LU632_RS08735 0.77 -0.0 1,641,317 + +0.6 1,641,318 - +1.5 1,641,318 - -0.2 1,641,351 - -0.7 1,641,398 + +0.7 1,641,398 + -0.5 1,641,399 - -1.0 1,641,426 + -0.1 1,641,447 + +1.3 1,641,447 + +1.5 1,641,448 - -0.2 1,641,483 + +0.4 1,641,572 + +0.2 1,641,581 + -0.8 1,641,581 + +0.8 1,641,604 + +0.1 1,641,604 + -0.7 1,641,608 + +0.8 1,641,608 + +0.2 1,641,717 + udk LU632_RS08740 0.22 +0.3 1,641,720 + udk LU632_RS08740 0.23 +0.5 1,641,764 + udk LU632_RS08740 0.30 -0.1 1,641,855 + udk LU632_RS08740 0.44 -0.7 1,641,855 + udk LU632_RS08740 0.44 +0.1 1,641,855 + udk LU632_RS08740 0.44 -0.4 1,641,855 + udk LU632_RS08740 0.44 +0.4 1,642,019 + udk LU632_RS08740 0.69 +1.2 1,642,121 + udk LU632_RS08740 0.85 -0.4 1,642,121 + udk LU632_RS08740 0.85 +0.0 1,642,128 + udk LU632_RS08740 0.86 +0.2 1,642,128 + udk LU632_RS08740 0.86 -0.3 1,642,139 + udk LU632_RS08740 0.88 -0.0 1,642,139 + udk LU632_RS08740 0.88 +0.3 1,642,236 + +0.2
Or see this region's nucleotide sequence