Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS08370 and LU632_RS08375 are separated by 246 nucleotides LU632_RS08375 and LU632_RS08380 overlap by 82 nucleotides LU632_RS08380 and LU632_RS08385 are separated by 113 nucleotides LU632_RS08385 and LU632_RS08390 overlap by 24 nucleotides LU632_RS08390 and LU632_RS08395 are separated by 112 nucleotides LU632_RS08395 and LU632_RS08400 are separated by 133 nucleotides
LU632_RS08370: LU632_RS08370 - IS5 family transposase, at 1,574,123 to 1,574,949
_RS08370
LU632_RS08375: LU632_RS08375 - IS1 family transposase, at 1,575,196 to 1,575,699
_RS08375
LU632_RS08380: LU632_RS08380 - IS1-like element transposase, at 1,575,618 to 1,575,893
_RS08380
LU632_RS08385: LU632_RS08385 - hypothetical protein, at 1,576,007 to 1,576,213
_RS08385
LU632_RS08390: LU632_RS08390 - hypothetical protein, at 1,576,190 to 1,576,402
_RS08390
LU632_RS08395: LU632_RS08395 - LacI family DNA-binding transcriptional regulator, at 1,576,515 to 1,576,640
_RS08395
LU632_RS08400: LU632_RS08400 - Rpn family recombination-promoting nuclease/putative transposase, at 1,576,774 to 1,577,526
_RS08400
Position (kb)
1575
1576 Strain fitness (log2 ratio)
-1
0
1 at 1574.959 kb on + strand at 1574.959 kb on + strand at 1574.959 kb on + strand at 1574.960 kb on - strand at 1574.984 kb on + strand at 1574.984 kb on + strand at 1574.985 kb on - strand at 1574.985 kb on - strand at 1574.986 kb on + strand at 1575.042 kb on + strand at 1575.042 kb on + strand at 1575.043 kb on - strand at 1575.043 kb on - strand at 1575.044 kb on + strand at 1575.044 kb on + strand at 1575.044 kb on + strand at 1575.095 kb on - strand at 1575.106 kb on + strand at 1575.107 kb on - strand at 1575.144 kb on - strand at 1575.146 kb on + strand at 1575.168 kb on - strand at 1575.973 kb on - strand at 1575.986 kb on - strand at 1576.016 kb on + strand at 1576.017 kb on - strand at 1576.018 kb on + strand at 1576.018 kb on + strand at 1576.033 kb on - strand, within LU632_RS08385 at 1576.127 kb on + strand, within LU632_RS08385 at 1576.128 kb on - strand, within LU632_RS08385 at 1576.340 kb on + strand, within LU632_RS08390 at 1576.353 kb on + strand, within LU632_RS08390 at 1576.353 kb on + strand, within LU632_RS08390 at 1576.353 kb on + strand, within LU632_RS08390 at 1576.353 kb on + strand, within LU632_RS08390 at 1576.353 kb on + strand, within LU632_RS08390 at 1576.354 kb on - strand, within LU632_RS08390 at 1576.354 kb on - strand, within LU632_RS08390 at 1576.354 kb on - strand, within LU632_RS08390 at 1576.366 kb on + strand, within LU632_RS08390 at 1576.435 kb on + strand at 1576.436 kb on - strand at 1576.477 kb on + strand at 1576.504 kb on + strand at 1576.622 kb on - strand, within LU632_RS08395 at 1576.652 kb on - strand at 1576.744 kb on + strand at 1576.744 kb on + strand at 1576.744 kb on + strand at 1576.744 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,574,959 + -0.0 1,574,959 + +0.3 1,574,959 + -0.0 1,574,960 - +0.3 1,574,984 + -0.4 1,574,984 + +0.4 1,574,985 - -0.7 1,574,985 - -0.2 1,574,986 + -0.4 1,575,042 + -0.8 1,575,042 + -0.2 1,575,043 - +0.4 1,575,043 - -0.6 1,575,044 + -0.2 1,575,044 + -0.2 1,575,044 + -1.6 1,575,095 - -0.4 1,575,106 + -0.8 1,575,107 - -1.0 1,575,144 - -1.0 1,575,146 + +0.1 1,575,168 - +0.3 1,575,973 - +0.3 1,575,986 - +0.0 1,576,016 + +0.0 1,576,017 - -0.0 1,576,018 + +0.0 1,576,018 + +0.4 1,576,033 - LU632_RS08385 0.13 -0.1 1,576,127 + LU632_RS08385 0.58 -0.5 1,576,128 - LU632_RS08385 0.58 +0.4 1,576,340 + LU632_RS08390 0.70 -1.4 1,576,353 + LU632_RS08390 0.77 -1.1 1,576,353 + LU632_RS08390 0.77 -0.2 1,576,353 + LU632_RS08390 0.77 +0.0 1,576,353 + LU632_RS08390 0.77 -0.5 1,576,353 + LU632_RS08390 0.77 -0.8 1,576,354 - LU632_RS08390 0.77 -0.8 1,576,354 - LU632_RS08390 0.77 +0.8 1,576,354 - LU632_RS08390 0.77 +0.4 1,576,366 + LU632_RS08390 0.83 +0.1 1,576,435 + -0.5 1,576,436 - -1.3 1,576,477 + -1.0 1,576,504 + +0.4 1,576,622 - LU632_RS08395 0.85 +1.2 1,576,652 - -0.0 1,576,744 + +0.7 1,576,744 + +0.6 1,576,744 + -0.7 1,576,744 + -0.3
Or see this region's nucleotide sequence