Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and LU632_RS06410 are separated by 12 nucleotides LU632_RS06410 and LU632_RS06415 are separated by 435 nucleotides
LU632_RS06405: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 1,231,332 to 1,233,047
cysI
LU632_RS06410: LU632_RS06410 - phosphoadenylyl-sulfate reductase, at 1,233,060 to 1,233,791
_RS06410
LU632_RS06415: LU632_RS06415 - hypothetical protein, at 1,234,227 to 1,234,436
_RS06415
Position (kb) 1233
1234 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1232.120 kb on + strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.123 kb on + strand, within cysI at 1232.134 kb on - strand, within cysI at 1232.259 kb on + strand, within cysI at 1232.610 kb on - strand, within cysI at 1232.747 kb on + strand, within cysI at 1232.747 kb on + strand, within cysI at 1232.748 kb on - strand, within cysI at 1232.835 kb on + strand, within cysI at 1232.838 kb on - strand, within cysI at 1232.873 kb on + strand, within cysI at 1232.883 kb on - strand at 1232.921 kb on + strand at 1233.318 kb on + strand, within LU632_RS06410 at 1233.451 kb on - strand, within LU632_RS06410 at 1233.684 kb on + strand, within LU632_RS06410 at 1233.702 kb on - strand, within LU632_RS06410 at 1233.789 kb on + strand at 1233.867 kb on - strand at 1233.867 kb on - strand at 1233.867 kb on - strand at 1233.963 kb on + strand at 1234.008 kb on - strand at 1234.084 kb on + strand at 1234.084 kb on + strand at 1234.087 kb on + strand at 1234.087 kb on + strand at 1234.101 kb on + strand at 1234.105 kb on + strand at 1234.125 kb on + strand at 1234.175 kb on - strand at 1234.177 kb on - strand at 1234.177 kb on - strand at 1234.181 kb on + strand at 1234.250 kb on - strand, within LU632_RS06415 at 1234.322 kb on + strand, within LU632_RS06415 at 1234.447 kb on + strand at 1234.447 kb on + strand at 1234.447 kb on + strand at 1234.465 kb on + strand at 1234.465 kb on + strand at 1234.465 kb on + strand at 1234.466 kb on - strand at 1234.466 kb on - strand at 1234.466 kb on - strand at 1234.466 kb on - strand at 1234.512 kb on - strand at 1234.514 kb on + strand at 1234.584 kb on - strand at 1234.584 kb on - strand at 1234.723 kb on + strand at 1234.735 kb on - strand at 1234.757 kb on - strand at 1234.778 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,232,120 + cysI LU632_RS06405 0.46 -1.5 1,232,121 - cysI LU632_RS06405 0.46 -3.6 1,232,121 - cysI LU632_RS06405 0.46 -3.4 1,232,121 - cysI LU632_RS06405 0.46 -2.1 1,232,123 + cysI LU632_RS06405 0.46 -2.1 1,232,134 - cysI LU632_RS06405 0.47 -2.9 1,232,259 + cysI LU632_RS06405 0.54 -2.3 1,232,610 - cysI LU632_RS06405 0.74 -2.1 1,232,747 + cysI LU632_RS06405 0.82 -3.4 1,232,747 + cysI LU632_RS06405 0.82 -4.0 1,232,748 - cysI LU632_RS06405 0.83 -2.9 1,232,835 + cysI LU632_RS06405 0.88 -3.3 1,232,838 - cysI LU632_RS06405 0.88 -2.3 1,232,873 + cysI LU632_RS06405 0.90 -3.1 1,232,883 - -1.3 1,232,921 + -1.9 1,233,318 + LU632_RS06410 0.35 -2.5 1,233,451 - LU632_RS06410 0.53 -1.3 1,233,684 + LU632_RS06410 0.85 -2.1 1,233,702 - LU632_RS06410 0.88 +0.1 1,233,789 + +0.3 1,233,867 - +0.9 1,233,867 - -0.0 1,233,867 - +0.8 1,233,963 + -1.1 1,234,008 - -0.3 1,234,084 + -1.1 1,234,084 + -0.1 1,234,087 + -1.9 1,234,087 + -0.3 1,234,101 + +0.5 1,234,105 + -1.2 1,234,125 + +0.1 1,234,175 - +0.1 1,234,177 - +0.6 1,234,177 - -0.1 1,234,181 + -0.5 1,234,250 - LU632_RS06415 0.11 -0.6 1,234,322 + LU632_RS06415 0.45 +0.5 1,234,447 + +1.4 1,234,447 + +0.1 1,234,447 + -0.5 1,234,465 + -0.0 1,234,465 + +0.6 1,234,465 + +0.2 1,234,466 - +0.1 1,234,466 - +0.0 1,234,466 - +0.8 1,234,466 - +1.5 1,234,512 - +0.4 1,234,514 + -0.8 1,234,584 - -0.5 1,234,584 - -1.6 1,234,723 + -2.3 1,234,735 - -2.8 1,234,757 - -2.3 1,234,778 + -0.9
Or see this region's nucleotide sequence