Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrG and eno are separated by 76 nucleotides eno and LU632_RS06375 are separated by 234 nucleotides
LU632_RS06365: pyrG - CTP synthase (glutamine hydrolyzing), at 1,222,994 to 1,224,631
pyrG
LU632_RS06370: eno - phosphopyruvate hydratase, at 1,224,708 to 1,226,003
eno
LU632_RS06375: LU632_RS06375 - acyltransferase, at 1,226,238 to 1,227,095
_RS06375
Position (kb)
1224
1225
1226
1227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1224.692 kb on + strand at 1226.190 kb on - strand at 1226.212 kb on - strand at 1226.275 kb on + strand at 1226.276 kb on - strand at 1226.276 kb on - strand at 1226.303 kb on - strand at 1226.340 kb on - strand, within LU632_RS06375 at 1226.388 kb on - strand, within LU632_RS06375 at 1226.419 kb on + strand, within LU632_RS06375 at 1226.464 kb on + strand, within LU632_RS06375 at 1226.464 kb on + strand, within LU632_RS06375 at 1226.464 kb on + strand, within LU632_RS06375 at 1226.465 kb on - strand, within LU632_RS06375 at 1226.496 kb on - strand, within LU632_RS06375 at 1226.515 kb on + strand, within LU632_RS06375 at 1226.516 kb on - strand, within LU632_RS06375 at 1226.528 kb on + strand, within LU632_RS06375 at 1226.530 kb on + strand, within LU632_RS06375 at 1226.592 kb on - strand, within LU632_RS06375 at 1226.622 kb on + strand, within LU632_RS06375 at 1226.622 kb on + strand, within LU632_RS06375 at 1226.622 kb on + strand, within LU632_RS06375 at 1226.622 kb on + strand, within LU632_RS06375 at 1226.623 kb on - strand, within LU632_RS06375 at 1226.623 kb on - strand, within LU632_RS06375 at 1226.624 kb on + strand, within LU632_RS06375 at 1226.630 kb on - strand, within LU632_RS06375 at 1226.708 kb on - strand, within LU632_RS06375 at 1226.708 kb on - strand, within LU632_RS06375 at 1226.760 kb on + strand, within LU632_RS06375 at 1226.760 kb on + strand, within LU632_RS06375 at 1226.761 kb on - strand, within LU632_RS06375 at 1226.762 kb on + strand, within LU632_RS06375 at 1226.762 kb on + strand, within LU632_RS06375 at 1226.763 kb on - strand, within LU632_RS06375 at 1226.854 kb on + strand, within LU632_RS06375 at 1226.888 kb on + strand, within LU632_RS06375 at 1226.998 kb on + strand, within LU632_RS06375
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,224,692 + +2.1 1,226,190 - -1.9 1,226,212 - +0.1 1,226,275 + +0.1 1,226,276 - -1.6 1,226,276 - +0.1 1,226,303 - +0.7 1,226,340 - LU632_RS06375 0.12 +0.5 1,226,388 - LU632_RS06375 0.17 +0.1 1,226,419 + LU632_RS06375 0.21 -0.4 1,226,464 + LU632_RS06375 0.26 +0.4 1,226,464 + LU632_RS06375 0.26 +0.1 1,226,464 + LU632_RS06375 0.26 +0.5 1,226,465 - LU632_RS06375 0.26 +0.1 1,226,496 - LU632_RS06375 0.30 -0.7 1,226,515 + LU632_RS06375 0.32 +0.5 1,226,516 - LU632_RS06375 0.32 -1.2 1,226,528 + LU632_RS06375 0.34 +0.9 1,226,530 + LU632_RS06375 0.34 -0.3 1,226,592 - LU632_RS06375 0.41 +0.1 1,226,622 + LU632_RS06375 0.45 +0.2 1,226,622 + LU632_RS06375 0.45 -0.3 1,226,622 + LU632_RS06375 0.45 -0.7 1,226,622 + LU632_RS06375 0.45 -0.7 1,226,623 - LU632_RS06375 0.45 +0.7 1,226,623 - LU632_RS06375 0.45 +1.6 1,226,624 + LU632_RS06375 0.45 -0.7 1,226,630 - LU632_RS06375 0.46 -0.2 1,226,708 - LU632_RS06375 0.55 +1.1 1,226,708 - LU632_RS06375 0.55 -0.7 1,226,760 + LU632_RS06375 0.61 +0.8 1,226,760 + LU632_RS06375 0.61 +0.1 1,226,761 - LU632_RS06375 0.61 -1.7 1,226,762 + LU632_RS06375 0.61 +0.5 1,226,762 + LU632_RS06375 0.61 -0.5 1,226,763 - LU632_RS06375 0.61 +0.3 1,226,854 + LU632_RS06375 0.72 -0.2 1,226,888 + LU632_RS06375 0.76 +0.7 1,226,998 + LU632_RS06375 0.89 -0.2
Or see this region's nucleotide sequence