Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiC and LU632_RS06220 are separated by 86 nucleotides LU632_RS06220 and mltA are separated by 204 nucleotides mltA and tcdA are separated by 53 nucleotides tcdA and csdE are separated by 0 nucleotides
LU632_RS06215: amiC - N-acetylmuramoyl-L-alanine amidase AmiC, at 1,191,094 to 1,192,323
amiC
LU632_RS06220: LU632_RS06220 - tRNA-Met, at 1,192,410 to 1,192,486
_RS06220
LU632_RS06225: mltA - murein transglycosylase A, at 1,192,691 to 1,193,833
mltA
LU632_RS06230: tcdA - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 1,193,887 to 1,194,687
tcdA
LU632_RS06235: csdE - cysteine desulfurase sulfur acceptor subunit CsdE, at 1,194,688 to 1,195,131
csdE
Position (kb) 1192
1193
1194 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1191.714 kb on + strand, within amiC at 1191.715 kb on - strand, within amiC at 1191.715 kb on - strand, within amiC at 1191.715 kb on - strand, within amiC at 1191.715 kb on - strand, within amiC at 1191.819 kb on - strand, within amiC at 1191.819 kb on - strand, within amiC at 1191.819 kb on - strand, within amiC at 1191.876 kb on + strand, within amiC at 1191.882 kb on + strand, within amiC at 1191.900 kb on + strand, within amiC at 1191.974 kb on - strand, within amiC at 1192.003 kb on - strand, within amiC at 1192.037 kb on - strand, within amiC at 1192.047 kb on + strand, within amiC at 1192.047 kb on + strand, within amiC at 1192.060 kb on - strand, within amiC at 1192.078 kb on - strand, within amiC at 1192.078 kb on - strand, within amiC at 1192.115 kb on + strand, within amiC at 1192.115 kb on + strand, within amiC at 1192.115 kb on + strand, within amiC at 1192.116 kb on - strand, within amiC at 1192.116 kb on - strand, within amiC at 1192.119 kb on + strand, within amiC at 1192.119 kb on + strand, within amiC at 1192.120 kb on - strand, within amiC at 1192.120 kb on - strand, within amiC at 1192.120 kb on - strand, within amiC at 1192.120 kb on - strand, within amiC at 1192.192 kb on - strand, within amiC at 1192.221 kb on + strand at 1192.221 kb on + strand at 1192.245 kb on + strand at 1192.245 kb on + strand at 1192.246 kb on - strand at 1192.246 kb on - strand at 1192.246 kb on - strand at 1192.301 kb on + strand at 1192.342 kb on - strand at 1192.342 kb on - strand at 1192.345 kb on + strand at 1192.346 kb on - strand at 1192.346 kb on - strand at 1192.347 kb on + strand at 1192.449 kb on + strand, within LU632_RS06220 at 1192.449 kb on + strand, within LU632_RS06220 at 1192.502 kb on + strand at 1192.503 kb on - strand at 1192.587 kb on + strand at 1192.588 kb on - strand at 1192.588 kb on - strand at 1192.653 kb on - strand at 1192.736 kb on - strand at 1192.785 kb on - strand at 1192.786 kb on + strand at 1192.787 kb on - strand at 1192.906 kb on + strand, within mltA at 1192.907 kb on - strand, within mltA at 1192.927 kb on + strand, within mltA at 1192.931 kb on + strand, within mltA at 1192.932 kb on - strand, within mltA at 1192.999 kb on + strand, within mltA at 1193.003 kb on + strand, within mltA at 1193.004 kb on - strand, within mltA at 1193.004 kb on - strand, within mltA at 1193.028 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.055 kb on - strand, within mltA at 1193.056 kb on + strand, within mltA at 1193.056 kb on + strand, within mltA at 1193.057 kb on - strand, within mltA at 1193.146 kb on + strand, within mltA at 1193.159 kb on + strand, within mltA at 1193.160 kb on - strand, within mltA at 1193.183 kb on + strand, within mltA at 1193.216 kb on - strand, within mltA at 1193.247 kb on - strand, within mltA at 1193.247 kb on - strand, within mltA at 1193.247 kb on - strand, within mltA at 1193.255 kb on + strand, within mltA at 1193.297 kb on - strand, within mltA at 1193.458 kb on + strand, within mltA at 1193.590 kb on + strand, within mltA at 1193.590 kb on + strand, within mltA at 1193.596 kb on + strand, within mltA at 1193.612 kb on + strand, within mltA at 1193.722 kb on + strand at 1193.729 kb on + strand at 1193.942 kb on - strand at 1193.979 kb on + strand, within tcdA at 1193.979 kb on + strand, within tcdA at 1193.980 kb on - strand, within tcdA at 1194.005 kb on + strand, within tcdA at 1194.005 kb on + strand, within tcdA at 1194.060 kb on + strand, within tcdA at 1194.198 kb on - strand, within tcdA at 1194.234 kb on - strand, within tcdA at 1194.234 kb on - strand, within tcdA at 1194.254 kb on - strand, within tcdA at 1194.319 kb on - strand, within tcdA at 1194.319 kb on - strand, within tcdA at 1194.333 kb on + strand, within tcdA at 1194.420 kb on + strand, within tcdA at 1194.425 kb on + strand, within tcdA at 1194.485 kb on + strand, within tcdA at 1194.485 kb on + strand, within tcdA at 1194.485 kb on + strand, within tcdA at 1194.517 kb on + strand, within tcdA at 1194.518 kb on - strand, within tcdA at 1194.654 kb on - strand at 1194.791 kb on - strand, within csdE at 1194.796 kb on + strand, within csdE
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,191,714 + amiC LU632_RS06215 0.50 +0.9 1,191,715 - amiC LU632_RS06215 0.50 +0.9 1,191,715 - amiC LU632_RS06215 0.50 +1.5 1,191,715 - amiC LU632_RS06215 0.50 -0.1 1,191,715 - amiC LU632_RS06215 0.50 -0.3 1,191,819 - amiC LU632_RS06215 0.59 +0.0 1,191,819 - amiC LU632_RS06215 0.59 +0.1 1,191,819 - amiC LU632_RS06215 0.59 +0.3 1,191,876 + amiC LU632_RS06215 0.64 +0.6 1,191,882 + amiC LU632_RS06215 0.64 -0.5 1,191,900 + amiC LU632_RS06215 0.66 -0.2 1,191,974 - amiC LU632_RS06215 0.72 -0.2 1,192,003 - amiC LU632_RS06215 0.74 -0.2 1,192,037 - amiC LU632_RS06215 0.77 -0.0 1,192,047 + amiC LU632_RS06215 0.77 -0.1 1,192,047 + amiC LU632_RS06215 0.77 +0.0 1,192,060 - amiC LU632_RS06215 0.79 -0.4 1,192,078 - amiC LU632_RS06215 0.80 -2.3 1,192,078 - amiC LU632_RS06215 0.80 +0.3 1,192,115 + amiC LU632_RS06215 0.83 -0.5 1,192,115 + amiC LU632_RS06215 0.83 -0.3 1,192,115 + amiC LU632_RS06215 0.83 -0.1 1,192,116 - amiC LU632_RS06215 0.83 -1.3 1,192,116 - amiC LU632_RS06215 0.83 -2.1 1,192,119 + amiC LU632_RS06215 0.83 -1.3 1,192,119 + amiC LU632_RS06215 0.83 -0.1 1,192,120 - amiC LU632_RS06215 0.83 +0.3 1,192,120 - amiC LU632_RS06215 0.83 +0.3 1,192,120 - amiC LU632_RS06215 0.83 -0.1 1,192,120 - amiC LU632_RS06215 0.83 +0.5 1,192,192 - amiC LU632_RS06215 0.89 +1.1 1,192,221 + +0.5 1,192,221 + +0.7 1,192,245 + -1.1 1,192,245 + +0.5 1,192,246 - -0.1 1,192,246 - +0.2 1,192,246 - +0.1 1,192,301 + +0.7 1,192,342 - +0.1 1,192,342 - +0.2 1,192,345 + +0.4 1,192,346 - +0.1 1,192,346 - +1.1 1,192,347 + -0.5 1,192,449 + LU632_RS06220 0.51 -1.9 1,192,449 + LU632_RS06220 0.51 +0.5 1,192,502 + -0.2 1,192,503 - -0.5 1,192,587 + +0.1 1,192,588 - +0.2 1,192,588 - -0.2 1,192,653 - -0.4 1,192,736 - +1.4 1,192,785 - -0.3 1,192,786 + +0.1 1,192,787 - -0.2 1,192,906 + mltA LU632_RS06225 0.19 +0.3 1,192,907 - mltA LU632_RS06225 0.19 +0.3 1,192,927 + mltA LU632_RS06225 0.21 -0.5 1,192,931 + mltA LU632_RS06225 0.21 -0.3 1,192,932 - mltA LU632_RS06225 0.21 +0.4 1,192,999 + mltA LU632_RS06225 0.27 +0.1 1,193,003 + mltA LU632_RS06225 0.27 -1.1 1,193,004 - mltA LU632_RS06225 0.27 +0.2 1,193,004 - mltA LU632_RS06225 0.27 +0.1 1,193,028 - mltA LU632_RS06225 0.29 -0.5 1,193,055 - mltA LU632_RS06225 0.32 -0.0 1,193,055 - mltA LU632_RS06225 0.32 -0.5 1,193,055 - mltA LU632_RS06225 0.32 -0.2 1,193,055 - mltA LU632_RS06225 0.32 +0.2 1,193,055 - mltA LU632_RS06225 0.32 -0.5 1,193,055 - mltA LU632_RS06225 0.32 +0.1 1,193,056 + mltA LU632_RS06225 0.32 -0.2 1,193,056 + mltA LU632_RS06225 0.32 +2.1 1,193,057 - mltA LU632_RS06225 0.32 -0.9 1,193,146 + mltA LU632_RS06225 0.40 -1.4 1,193,159 + mltA LU632_RS06225 0.41 +0.3 1,193,160 - mltA LU632_RS06225 0.41 +0.3 1,193,183 + mltA LU632_RS06225 0.43 -1.3 1,193,216 - mltA LU632_RS06225 0.46 -0.4 1,193,247 - mltA LU632_RS06225 0.49 -0.2 1,193,247 - mltA LU632_RS06225 0.49 -0.4 1,193,247 - mltA LU632_RS06225 0.49 -0.9 1,193,255 + mltA LU632_RS06225 0.49 -0.9 1,193,297 - mltA LU632_RS06225 0.53 +0.3 1,193,458 + mltA LU632_RS06225 0.67 -0.5 1,193,590 + mltA LU632_RS06225 0.79 -0.8 1,193,590 + mltA LU632_RS06225 0.79 +0.5 1,193,596 + mltA LU632_RS06225 0.79 -0.6 1,193,612 + mltA LU632_RS06225 0.81 +0.1 1,193,722 + +0.1 1,193,729 + +0.3 1,193,942 - +0.2 1,193,979 + tcdA LU632_RS06230 0.11 +0.0 1,193,979 + tcdA LU632_RS06230 0.11 +0.2 1,193,980 - tcdA LU632_RS06230 0.12 +0.1 1,194,005 + tcdA LU632_RS06230 0.15 +0.3 1,194,005 + tcdA LU632_RS06230 0.15 -0.0 1,194,060 + tcdA LU632_RS06230 0.22 -0.1 1,194,198 - tcdA LU632_RS06230 0.39 +0.8 1,194,234 - tcdA LU632_RS06230 0.43 -1.1 1,194,234 - tcdA LU632_RS06230 0.43 -0.5 1,194,254 - tcdA LU632_RS06230 0.46 +0.5 1,194,319 - tcdA LU632_RS06230 0.54 -3.0 1,194,319 - tcdA LU632_RS06230 0.54 -0.9 1,194,333 + tcdA LU632_RS06230 0.56 -0.5 1,194,420 + tcdA LU632_RS06230 0.67 +0.1 1,194,425 + tcdA LU632_RS06230 0.67 +1.6 1,194,485 + tcdA LU632_RS06230 0.75 +0.2 1,194,485 + tcdA LU632_RS06230 0.75 -0.0 1,194,485 + tcdA LU632_RS06230 0.75 -0.4 1,194,517 + tcdA LU632_RS06230 0.79 -0.5 1,194,518 - tcdA LU632_RS06230 0.79 -2.0 1,194,654 - +1.4 1,194,791 - csdE LU632_RS06235 0.23 +0.5 1,194,796 + csdE LU632_RS06235 0.24 +0.6
Or see this region's nucleotide sequence