Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS06030 and LU632_RS06035 overlap by 4 nucleotides LU632_RS06035 and lysA are separated by 117 nucleotides
LU632_RS06030: LU632_RS06030 - MFS transporter, at 1,144,299 to 1,145,374
_RS06030
LU632_RS06035: LU632_RS06035 - LysR family transcriptional regulator, at 1,145,371 to 1,146,291
_RS06035
LU632_RS06040: lysA - diaminopimelate decarboxylase, at 1,146,409 to 1,147,668
lysA
Position (kb)
1145
1146
1147 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1144.401 kb on + strand at 1144.618 kb on + strand, within LU632_RS06030 at 1144.677 kb on + strand, within LU632_RS06030 at 1144.677 kb on + strand, within LU632_RS06030 at 1144.677 kb on + strand, within LU632_RS06030 at 1144.743 kb on + strand, within LU632_RS06030 at 1144.743 kb on + strand, within LU632_RS06030 at 1144.747 kb on + strand, within LU632_RS06030 at 1144.747 kb on + strand, within LU632_RS06030 at 1144.747 kb on + strand, within LU632_RS06030 at 1144.821 kb on + strand, within LU632_RS06030 at 1144.837 kb on + strand, within LU632_RS06030 at 1144.837 kb on + strand, within LU632_RS06030 at 1144.871 kb on + strand, within LU632_RS06030 at 1144.871 kb on + strand, within LU632_RS06030 at 1144.871 kb on + strand, within LU632_RS06030 at 1144.871 kb on + strand, within LU632_RS06030 at 1144.872 kb on - strand, within LU632_RS06030 at 1144.872 kb on - strand, within LU632_RS06030 at 1144.873 kb on + strand, within LU632_RS06030 at 1144.873 kb on + strand, within LU632_RS06030 at 1144.966 kb on + strand, within LU632_RS06030 at 1144.966 kb on + strand, within LU632_RS06030 at 1144.966 kb on + strand, within LU632_RS06030 at 1144.967 kb on - strand, within LU632_RS06030 at 1145.137 kb on - strand, within LU632_RS06030 at 1145.255 kb on - strand, within LU632_RS06030 at 1145.262 kb on - strand, within LU632_RS06030 at 1145.313 kb on + strand at 1145.313 kb on + strand at 1145.314 kb on - strand at 1145.372 kb on + strand at 1145.412 kb on + strand at 1145.412 kb on + strand at 1145.490 kb on + strand, within LU632_RS06035 at 1145.574 kb on + strand, within LU632_RS06035 at 1145.711 kb on + strand, within LU632_RS06035 at 1145.712 kb on - strand, within LU632_RS06035 at 1145.910 kb on + strand, within LU632_RS06035 at 1145.911 kb on - strand, within LU632_RS06035 at 1145.913 kb on + strand, within LU632_RS06035 at 1145.914 kb on - strand, within LU632_RS06035 at 1146.097 kb on + strand, within LU632_RS06035 at 1146.268 kb on - strand at 1146.333 kb on - strand at 1146.333 kb on - strand at 1146.379 kb on + strand at 1146.436 kb on - strand at 1146.444 kb on - strand at 1146.499 kb on + strand at 1146.500 kb on - strand at 1146.537 kb on - strand, within lysA at 1146.537 kb on - strand, within lysA at 1146.723 kb on + strand, within lysA at 1146.723 kb on + strand, within lysA at 1146.724 kb on - strand, within lysA at 1146.724 kb on - strand, within lysA at 1146.994 kb on + strand, within lysA at 1147.132 kb on + strand, within lysA at 1147.158 kb on + strand, within lysA at 1147.165 kb on - strand, within lysA
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,144,401 + +0.4 1,144,618 + LU632_RS06030 0.30 +0.3 1,144,677 + LU632_RS06030 0.35 -0.8 1,144,677 + LU632_RS06030 0.35 +0.2 1,144,677 + LU632_RS06030 0.35 -0.2 1,144,743 + LU632_RS06030 0.41 +1.1 1,144,743 + LU632_RS06030 0.41 +0.7 1,144,747 + LU632_RS06030 0.42 -0.6 1,144,747 + LU632_RS06030 0.42 -0.6 1,144,747 + LU632_RS06030 0.42 +0.5 1,144,821 + LU632_RS06030 0.49 -0.2 1,144,837 + LU632_RS06030 0.50 -0.7 1,144,837 + LU632_RS06030 0.50 +0.0 1,144,871 + LU632_RS06030 0.53 -0.3 1,144,871 + LU632_RS06030 0.53 -0.2 1,144,871 + LU632_RS06030 0.53 +0.3 1,144,871 + LU632_RS06030 0.53 +0.3 1,144,872 - LU632_RS06030 0.53 -0.5 1,144,872 - LU632_RS06030 0.53 +0.0 1,144,873 + LU632_RS06030 0.53 +0.4 1,144,873 + LU632_RS06030 0.53 +0.4 1,144,966 + LU632_RS06030 0.62 +0.1 1,144,966 + LU632_RS06030 0.62 -0.2 1,144,966 + LU632_RS06030 0.62 +0.9 1,144,967 - LU632_RS06030 0.62 +0.1 1,145,137 - LU632_RS06030 0.78 +0.9 1,145,255 - LU632_RS06030 0.89 -1.0 1,145,262 - LU632_RS06030 0.89 +0.3 1,145,313 + -1.7 1,145,313 + +0.2 1,145,314 - +0.1 1,145,372 + -0.2 1,145,412 + -0.3 1,145,412 + -0.3 1,145,490 + LU632_RS06035 0.13 +0.1 1,145,574 + LU632_RS06035 0.22 -0.6 1,145,711 + LU632_RS06035 0.37 +0.3 1,145,712 - LU632_RS06035 0.37 -0.1 1,145,910 + LU632_RS06035 0.59 -1.8 1,145,911 - LU632_RS06035 0.59 +0.6 1,145,913 + LU632_RS06035 0.59 +0.3 1,145,914 - LU632_RS06035 0.59 +0.1 1,146,097 + LU632_RS06035 0.79 -0.5 1,146,268 - -0.3 1,146,333 - +0.3 1,146,333 - -0.2 1,146,379 + +0.0 1,146,436 - -4.4 1,146,444 - -3.6 1,146,499 + -4.3 1,146,500 - -0.5 1,146,537 - lysA LU632_RS06040 0.10 -0.9 1,146,537 - lysA LU632_RS06040 0.10 -2.1 1,146,723 + lysA LU632_RS06040 0.25 -2.4 1,146,723 + lysA LU632_RS06040 0.25 -5.4 1,146,724 - lysA LU632_RS06040 0.25 -4.0 1,146,724 - lysA LU632_RS06040 0.25 -0.5 1,146,994 + lysA LU632_RS06040 0.46 -3.9 1,147,132 + lysA LU632_RS06040 0.57 -1.9 1,147,158 + lysA LU632_RS06040 0.59 -0.9 1,147,165 - lysA LU632_RS06040 0.60 +1.1
Or see this region's nucleotide sequence