Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS05820 and LU632_RS05825 overlap by 14 nucleotides LU632_RS05825 and LU632_RS05830 are separated by 80 nucleotides LU632_RS05830 and LU632_RS05835 are separated by 411 nucleotides
LU632_RS05820: LU632_RS05820 - Mu transposase C-terminal domain-containing protein, at 1,104,278 to 1,106,260
_RS05820
LU632_RS05825: LU632_RS05825 - hypothetical protein, at 1,106,247 to 1,106,471
_RS05825
LU632_RS05830: LU632_RS05830 - helix-turn-helix transcriptional regulator, at 1,106,552 to 1,107,373
_RS05830
LU632_RS05835: LU632_RS05835 - hypothetical protein, at 1,107,785 to 1,108,168
_RS05835
Position (kb)
1106
1107
1108 Strain fitness (log2 ratio)
-1
0
1 at 1107.821 kb on - strand at 1107.873 kb on + strand, within LU632_RS05835 at 1108.039 kb on + strand, within LU632_RS05835 at 1108.040 kb on - strand, within LU632_RS05835 at 1108.048 kb on + strand, within LU632_RS05835 at 1108.066 kb on + strand, within LU632_RS05835 at 1108.155 kb on + strand at 1108.158 kb on + strand at 1108.167 kb on - strand at 1108.235 kb on - strand at 1108.254 kb on + strand at 1108.279 kb on + strand at 1108.279 kb on + strand at 1108.291 kb on - strand at 1108.291 kb on - strand at 1108.327 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,107,821 - +0.4 1,107,873 + LU632_RS05835 0.23 +0.2 1,108,039 + LU632_RS05835 0.66 +0.3 1,108,040 - LU632_RS05835 0.66 +0.1 1,108,048 + LU632_RS05835 0.68 +0.5 1,108,066 + LU632_RS05835 0.73 -0.1 1,108,155 + -1.7 1,108,158 + -0.3 1,108,167 - -0.3 1,108,235 - +0.8 1,108,254 + -0.2 1,108,279 + +0.6 1,108,279 + -0.8 1,108,291 - -0.3 1,108,291 - -1.5 1,108,327 + -0.3
Or see this region's nucleotide sequence