Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mscS and tnpA are separated by 171 nucleotides tnpA and LU632_RS05560 are separated by 191 nucleotides LU632_RS05560 and LU632_RS05565 are separated by 25 nucleotides
LU632_RS05550: mscS - small-conductance mechanosensitive channel MscS, at 1,075,203 to 1,076,087
mscS
LU632_RS05555: tnpA - IS200/IS605 family transposase, at 1,076,259 to 1,076,689
tnpA
LU632_RS05560: LU632_RS05560 - LysE family transporter, at 1,076,881 to 1,077,492
_RS05560
LU632_RS05565: LU632_RS05565 - hypothetical protein, at 1,077,518 to 1,077,832
_RS05565
Position (kb)
1076
1077 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1075.281 kb on + strand at 1075.534 kb on + strand, within mscS at 1075.608 kb on - strand, within mscS at 1075.764 kb on - strand, within mscS at 1075.811 kb on - strand, within mscS at 1075.844 kb on - strand, within mscS at 1076.000 kb on + strand at 1076.001 kb on - strand at 1076.001 kb on - strand at 1076.002 kb on + strand at 1076.003 kb on - strand at 1076.040 kb on - strand at 1076.086 kb on - strand at 1076.102 kb on - strand at 1076.894 kb on - strand at 1076.912 kb on + strand at 1076.912 kb on + strand at 1076.951 kb on + strand, within LU632_RS05560 at 1076.972 kb on + strand, within LU632_RS05560 at 1077.024 kb on + strand, within LU632_RS05560 at 1077.024 kb on + strand, within LU632_RS05560 at 1077.024 kb on + strand, within LU632_RS05560 at 1077.025 kb on - strand, within LU632_RS05560 at 1077.025 kb on - strand, within LU632_RS05560 at 1077.027 kb on - strand, within LU632_RS05560 at 1077.027 kb on - strand, within LU632_RS05560 at 1077.027 kb on - strand, within LU632_RS05560 at 1077.084 kb on - strand, within LU632_RS05560 at 1077.213 kb on + strand, within LU632_RS05560 at 1077.264 kb on + strand, within LU632_RS05560 at 1077.484 kb on + strand at 1077.488 kb on + strand at 1077.488 kb on + strand at 1077.489 kb on - strand at 1077.513 kb on - strand at 1077.514 kb on + strand at 1077.514 kb on + strand at 1077.514 kb on + strand at 1077.514 kb on + strand at 1077.514 kb on + strand at 1077.577 kb on + strand, within LU632_RS05565 at 1077.578 kb on - strand, within LU632_RS05565 at 1077.659 kb on + strand, within LU632_RS05565 at 1077.659 kb on + strand, within LU632_RS05565 at 1077.659 kb on + strand, within LU632_RS05565 at 1077.660 kb on - strand, within LU632_RS05565
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,075,281 + -0.1 1,075,534 + mscS LU632_RS05550 0.37 +0.6 1,075,608 - mscS LU632_RS05550 0.46 +0.1 1,075,764 - mscS LU632_RS05550 0.63 +1.3 1,075,811 - mscS LU632_RS05550 0.69 +0.8 1,075,844 - mscS LU632_RS05550 0.72 +0.1 1,076,000 + -0.7 1,076,001 - +0.9 1,076,001 - -0.7 1,076,002 + -0.2 1,076,003 - -0.1 1,076,040 - +0.5 1,076,086 - -0.3 1,076,102 - -0.1 1,076,894 - +0.6 1,076,912 + +0.2 1,076,912 + +1.5 1,076,951 + LU632_RS05560 0.11 +1.4 1,076,972 + LU632_RS05560 0.15 -0.7 1,077,024 + LU632_RS05560 0.23 +0.3 1,077,024 + LU632_RS05560 0.23 -2.8 1,077,024 + LU632_RS05560 0.23 +0.1 1,077,025 - LU632_RS05560 0.24 -0.2 1,077,025 - LU632_RS05560 0.24 +0.4 1,077,027 - LU632_RS05560 0.24 +0.2 1,077,027 - LU632_RS05560 0.24 +0.3 1,077,027 - LU632_RS05560 0.24 +0.0 1,077,084 - LU632_RS05560 0.33 -0.9 1,077,213 + LU632_RS05560 0.54 -0.0 1,077,264 + LU632_RS05560 0.63 +1.8 1,077,484 + +1.0 1,077,488 + -0.1 1,077,488 + -0.3 1,077,489 - +0.2 1,077,513 - +0.6 1,077,514 + +0.7 1,077,514 + -1.4 1,077,514 + -2.0 1,077,514 + +0.1 1,077,514 + +0.4 1,077,577 + LU632_RS05565 0.19 +0.8 1,077,578 - LU632_RS05565 0.19 +0.8 1,077,659 + LU632_RS05565 0.45 +0.7 1,077,659 + LU632_RS05565 0.45 -0.2 1,077,659 + LU632_RS05565 0.45 -0.5 1,077,660 - LU632_RS05565 0.45 -0.2
Or see this region's nucleotide sequence