Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04515 and LU632_RS04520 are separated by 109 nucleotides LU632_RS04520 and LU632_RS04525 are separated by 376 nucleotides LU632_RS04525 and LU632_RS04530 overlap by 11 nucleotides LU632_RS04530 and LU632_RS04535 are separated by 343 nucleotides
LU632_RS04515: LU632_RS04515 - DUF4158 domain-containing protein, at 887,038 to 887,541
_RS04515
LU632_RS04520: LU632_RS04520 - hypothetical protein, at 887,651 to 887,941
_RS04520
LU632_RS04525: LU632_RS04525 - type II toxin-antitoxin system RelB/DinJ family antitoxin, at 888,318 to 888,572
_RS04525
LU632_RS04530: LU632_RS04530 - type II toxin-antitoxin system RelE/ParE family toxin, at 888,562 to 888,852
_RS04530
LU632_RS04535: LU632_RS04535 - IS91 family transposase, at 889,196 to 890,419
_RS04535
Position (kb) 888
889 Strain fitness (log2 ratio) -1
0
1
2 at 887.627 kb on + strand at 888.166 kb on + strand at 888.166 kb on + strand at 888.166 kb on + strand at 888.166 kb on + strand at 888.167 kb on - strand at 888.167 kb on - strand at 888.167 kb on - strand at 888.173 kb on + strand at 888.173 kb on + strand at 888.174 kb on - strand at 888.177 kb on + strand at 888.178 kb on - strand at 888.190 kb on + strand at 888.609 kb on + strand, within LU632_RS04530 at 888.609 kb on + strand, within LU632_RS04530 at 888.609 kb on + strand, within LU632_RS04530 at 888.782 kb on + strand, within LU632_RS04530 at 888.783 kb on - strand, within LU632_RS04530 at 888.851 kb on - strand at 888.915 kb on - strand at 888.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 887,627 + +0.3 888,166 + -0.6 888,166 + +0.2 888,166 + -0.7 888,166 + +0.1 888,167 - -0.1 888,167 - +1.1 888,167 - +0.1 888,173 + +0.1 888,173 + +1.9 888,174 - +0.0 888,177 + -0.2 888,178 - +0.1 888,190 + -0.1 888,609 + LU632_RS04530 0.16 -0.4 888,609 + LU632_RS04530 0.16 +0.0 888,609 + LU632_RS04530 0.16 +0.7 888,782 + LU632_RS04530 0.76 -0.7 888,783 - LU632_RS04530 0.76 +0.2 888,851 - +0.1 888,915 - -0.6 888,954 + -0.5
Or see this region's nucleotide sequence