Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS02075 and LU632_RS02080 are separated by 146 nucleotides LU632_RS02080 and cysQ are separated by 312 nucleotides cysQ and LU632_RS02090 are separated by 41 nucleotides LU632_RS02090 and LU632_RS02095 are separated by 303 nucleotides
LU632_RS02075: LU632_RS02075 - transposase, at 408,670 to 409,005
_RS02075
LU632_RS02080: LU632_RS02080 - hypothetical protein, at 409,152 to 409,565
_RS02080
LU632_RS02085: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 409,878 to 410,618
cysQ
LU632_RS02090: LU632_RS02090 - YtfJ family protein, at 410,660 to 411,242
_RS02090
LU632_RS02095: LU632_RS02095 - DUF1107 domain-containing protein, at 411,546 to 411,752
_RS02095
Position (kb) 409
410
411 Strain fitness (log2 ratio) -2
-1
0
1
2 at 408.878 kb on + strand, within LU632_RS02075 at 408.878 kb on + strand, within LU632_RS02075 at 408.956 kb on - strand, within LU632_RS02075 at 409.065 kb on - strand at 409.068 kb on + strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.098 kb on - strand at 409.106 kb on + strand at 409.130 kb on + strand at 409.138 kb on - strand at 409.165 kb on - strand at 409.221 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.272 kb on - strand, within LU632_RS02080 at 409.315 kb on - strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.499 kb on - strand, within LU632_RS02080 at 409.560 kb on + strand at 409.561 kb on - strand at 409.561 kb on - strand at 409.607 kb on - strand at 409.639 kb on - strand at 409.704 kb on - strand at 409.833 kb on + strand at 409.833 kb on + strand at 409.834 kb on - strand at 409.834 kb on - strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.860 kb on + strand at 409.860 kb on + strand at 409.861 kb on - strand at 409.861 kb on - strand at 409.933 kb on + strand at 409.933 kb on + strand at 409.934 kb on - strand at 409.935 kb on + strand at 409.935 kb on + strand at 409.936 kb on - strand at 410.244 kb on - strand, within cysQ at 410.477 kb on + strand, within cysQ at 410.477 kb on + strand, within cysQ at 410.566 kb on - strand at 410.566 kb on - strand at 410.566 kb on - strand at 410.791 kb on + strand, within LU632_RS02090 at 410.855 kb on - strand, within LU632_RS02090 at 410.897 kb on - strand, within LU632_RS02090 at 411.002 kb on + strand, within LU632_RS02090 at 411.043 kb on + strand, within LU632_RS02090 at 411.124 kb on - strand, within LU632_RS02090 at 411.168 kb on + strand, within LU632_RS02090 at 411.171 kb on - strand, within LU632_RS02090 at 411.263 kb on + strand at 411.330 kb on - strand at 411.330 kb on - strand at 411.339 kb on + strand at 411.370 kb on + strand at 411.382 kb on + strand at 411.383 kb on - strand at 411.468 kb on + strand at 411.468 kb on + strand at 411.584 kb on + strand, within LU632_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 408,878 + LU632_RS02075 0.62 +1.8 408,878 + LU632_RS02075 0.62 -0.2 408,956 - LU632_RS02075 0.85 +0.7 409,065 - +0.5 409,068 + -0.1 409,069 - +0.4 409,069 - -0.5 409,069 - +0.1 409,098 - -0.5 409,106 + +1.0 409,130 + -0.1 409,138 - +0.8 409,165 - +0.0 409,221 - LU632_RS02080 0.17 +0.7 409,223 - LU632_RS02080 0.17 -0.3 409,223 - LU632_RS02080 0.17 -0.7 409,223 - LU632_RS02080 0.17 -0.2 409,272 - LU632_RS02080 0.29 +0.0 409,315 - LU632_RS02080 0.39 -0.6 409,335 + LU632_RS02080 0.44 +0.3 409,335 + LU632_RS02080 0.44 -0.1 409,335 + LU632_RS02080 0.44 +0.4 409,336 - LU632_RS02080 0.44 -0.6 409,336 - LU632_RS02080 0.44 -0.3 409,498 + LU632_RS02080 0.84 -0.6 409,498 + LU632_RS02080 0.84 +0.1 409,499 - LU632_RS02080 0.84 -1.0 409,560 + +0.5 409,561 - +0.0 409,561 - -0.0 409,607 - +0.2 409,639 - -0.4 409,704 - +0.5 409,833 + -0.6 409,833 + +0.1 409,834 - -1.8 409,834 - -0.8 409,838 - -2.1 409,838 - -1.1 409,860 + +0.1 409,860 + +0.2 409,861 - -1.2 409,861 - -1.3 409,933 + -1.8 409,933 + -2.1 409,934 - -1.8 409,935 + -2.6 409,935 + -1.3 409,936 - -1.1 410,244 - cysQ LU632_RS02085 0.49 -1.7 410,477 + cysQ LU632_RS02085 0.81 +0.2 410,477 + cysQ LU632_RS02085 0.81 -1.3 410,566 - +1.2 410,566 - -1.1 410,566 - -1.5 410,791 + LU632_RS02090 0.22 -0.0 410,855 - LU632_RS02090 0.33 -0.1 410,897 - LU632_RS02090 0.41 -0.6 411,002 + LU632_RS02090 0.59 +0.0 411,043 + LU632_RS02090 0.66 -0.1 411,124 - LU632_RS02090 0.80 -0.2 411,168 + LU632_RS02090 0.87 +0.1 411,171 - LU632_RS02090 0.88 -0.4 411,263 + +1.2 411,330 - -0.5 411,330 - -0.7 411,339 + -0.6 411,370 + +0.2 411,382 + -0.0 411,383 - +0.5 411,468 + -0.5 411,468 + -0.1 411,584 + LU632_RS02095 0.18 -0.3
Or see this region's nucleotide sequence