Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflC and LU632_RS02075 are separated by 481 nucleotides LU632_RS02075 and LU632_RS02080 are separated by 146 nucleotides LU632_RS02080 and cysQ are separated by 312 nucleotides
LU632_RS02070: hflC - protease modulator HflC, at 407,178 to 408,188
hflC
LU632_RS02075: LU632_RS02075 - transposase, at 408,670 to 409,005
_RS02075
LU632_RS02080: LU632_RS02080 - hypothetical protein, at 409,152 to 409,565
_RS02080
LU632_RS02085: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 409,878 to 410,618
cysQ
Position (kb) 408
409
410 Strain fitness (log2 ratio) -2
-1
0
1
2 at 407.847 kb on + strand, within hflC at 408.057 kb on + strand, within hflC at 408.058 kb on - strand, within hflC at 408.065 kb on + strand, within hflC at 408.179 kb on + strand at 408.186 kb on + strand at 408.187 kb on - strand at 408.222 kb on + strand at 408.223 kb on - strand at 408.314 kb on + strand at 408.337 kb on + strand at 408.347 kb on + strand at 408.348 kb on - strand at 408.402 kb on + strand at 408.425 kb on + strand at 408.425 kb on + strand at 408.425 kb on - strand at 408.426 kb on - strand at 408.426 kb on - strand at 408.426 kb on - strand at 408.426 kb on - strand at 408.426 kb on - strand at 408.426 kb on - strand at 408.557 kb on + strand at 408.557 kb on + strand at 408.558 kb on - strand at 408.581 kb on - strand at 408.600 kb on + strand at 408.659 kb on - strand at 408.705 kb on + strand, within LU632_RS02075 at 408.878 kb on + strand, within LU632_RS02075 at 408.878 kb on + strand, within LU632_RS02075 at 408.956 kb on - strand, within LU632_RS02075 at 409.065 kb on - strand at 409.068 kb on + strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.098 kb on - strand at 409.106 kb on + strand at 409.130 kb on + strand at 409.138 kb on - strand at 409.165 kb on - strand at 409.221 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.272 kb on - strand, within LU632_RS02080 at 409.315 kb on - strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.499 kb on - strand, within LU632_RS02080 at 409.560 kb on + strand at 409.561 kb on - strand at 409.561 kb on - strand at 409.607 kb on - strand at 409.639 kb on - strand at 409.704 kb on - strand at 409.833 kb on + strand at 409.833 kb on + strand at 409.834 kb on - strand at 409.834 kb on - strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.860 kb on + strand at 409.860 kb on + strand at 409.861 kb on - strand at 409.861 kb on - strand at 409.933 kb on + strand at 409.933 kb on + strand at 409.934 kb on - strand at 409.935 kb on + strand at 409.935 kb on + strand at 409.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 407,847 + hflC LU632_RS02070 0.66 -2.0 408,057 + hflC LU632_RS02070 0.87 -0.3 408,058 - hflC LU632_RS02070 0.87 -1.6 408,065 + hflC LU632_RS02070 0.88 -2.1 408,179 + +0.2 408,186 + +0.7 408,187 - -0.5 408,222 + -0.1 408,223 - +0.7 408,314 + -0.2 408,337 + -0.3 408,347 + +0.5 408,348 - -0.8 408,402 + -0.3 408,425 + -0.5 408,425 + -0.4 408,425 - +0.1 408,426 - +0.4 408,426 - +0.0 408,426 - +0.1 408,426 - -0.5 408,426 - -0.3 408,426 - -0.7 408,557 + -0.1 408,557 + -0.0 408,558 - -0.2 408,581 - +0.3 408,600 + +0.2 408,659 - +0.7 408,705 + LU632_RS02075 0.10 -0.4 408,878 + LU632_RS02075 0.62 +1.8 408,878 + LU632_RS02075 0.62 -0.2 408,956 - LU632_RS02075 0.85 +0.7 409,065 - +0.5 409,068 + -0.1 409,069 - +0.4 409,069 - -0.5 409,069 - +0.1 409,098 - -0.5 409,106 + +1.0 409,130 + -0.1 409,138 - +0.8 409,165 - +0.0 409,221 - LU632_RS02080 0.17 +0.7 409,223 - LU632_RS02080 0.17 -0.3 409,223 - LU632_RS02080 0.17 -0.7 409,223 - LU632_RS02080 0.17 -0.2 409,272 - LU632_RS02080 0.29 +0.0 409,315 - LU632_RS02080 0.39 -0.6 409,335 + LU632_RS02080 0.44 +0.3 409,335 + LU632_RS02080 0.44 -0.1 409,335 + LU632_RS02080 0.44 +0.4 409,336 - LU632_RS02080 0.44 -0.6 409,336 - LU632_RS02080 0.44 -0.3 409,498 + LU632_RS02080 0.84 -0.6 409,498 + LU632_RS02080 0.84 +0.1 409,499 - LU632_RS02080 0.84 -1.0 409,560 + +0.5 409,561 - +0.0 409,561 - -0.0 409,607 - +0.2 409,639 - -0.4 409,704 - +0.5 409,833 + -0.6 409,833 + +0.1 409,834 - -1.8 409,834 - -0.8 409,838 - -2.1 409,838 - -1.1 409,860 + +0.1 409,860 + +0.2 409,861 - -1.2 409,861 - -1.3 409,933 + -1.8 409,933 + -2.1 409,934 - -1.8 409,935 + -2.6 409,935 + -1.3 409,936 - -1.1
Or see this region's nucleotide sequence