Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS01845 and LU632_RS01850 are separated by 115 nucleotides LU632_RS01850 and aspA are separated by 4 nucleotides aspA and LU632_RS01860 are separated by 347 nucleotides
LU632_RS01845: LU632_RS01845 - anaerobic C4-dicarboxylate transporter, at 355,557 to 356,858
_RS01845
LU632_RS01850: LU632_RS01850 - hypothetical protein, at 356,974 to 357,117
_RS01850
LU632_RS01855: aspA - aspartate ammonia-lyase, at 357,122 to 357,357
aspA
LU632_RS01860: LU632_RS01860 - FxsA family protein, at 357,705 to 358,160
_RS01860
Position (kb)
356
357
358 Strain fitness (log2 ratio)
-2
-1
0
1 at 356.042 kb on + strand, within LU632_RS01845 at 356.043 kb on - strand, within LU632_RS01845 at 356.070 kb on + strand, within LU632_RS01845 at 356.070 kb on + strand, within LU632_RS01845 at 356.071 kb on - strand, within LU632_RS01845 at 356.091 kb on + strand, within LU632_RS01845 at 356.521 kb on - strand, within LU632_RS01845 at 356.521 kb on - strand, within LU632_RS01845 at 356.567 kb on + strand, within LU632_RS01845 at 356.567 kb on + strand, within LU632_RS01845 at 356.568 kb on - strand, within LU632_RS01845 at 356.568 kb on - strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.569 kb on + strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.570 kb on - strand, within LU632_RS01845 at 356.573 kb on + strand, within LU632_RS01845 at 356.700 kb on - strand, within LU632_RS01845 at 356.736 kb on + strand at 356.841 kb on + strand at 356.841 kb on + strand at 356.842 kb on - strand at 356.842 kb on - strand at 356.842 kb on - strand at 356.902 kb on - strand at 356.962 kb on + strand at 356.963 kb on - strand at 357.040 kb on + strand, within LU632_RS01850 at 357.041 kb on - strand, within LU632_RS01850 at 357.041 kb on - strand, within LU632_RS01850 at 357.041 kb on - strand, within LU632_RS01850 at 357.041 kb on - strand, within LU632_RS01850 at 357.041 kb on - strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.042 kb on + strand, within LU632_RS01850 at 357.043 kb on - strand, within LU632_RS01850 at 357.043 kb on - strand, within LU632_RS01850 at 357.043 kb on - strand, within LU632_RS01850 at 357.118 kb on + strand at 357.118 kb on + strand at 357.119 kb on - strand at 357.119 kb on - strand at 357.119 kb on - strand at 357.119 kb on - strand at 357.119 kb on - strand at 357.119 kb on - strand at 357.121 kb on - strand at 357.121 kb on - strand at 357.121 kb on - strand at 357.140 kb on - strand at 357.229 kb on + strand, within aspA at 357.242 kb on - strand, within aspA at 357.280 kb on + strand, within aspA at 357.281 kb on - strand, within aspA at 357.291 kb on + strand, within aspA at 357.295 kb on - strand, within aspA at 357.295 kb on - strand, within aspA at 357.467 kb on - strand at 357.485 kb on + strand at 357.486 kb on - strand at 357.508 kb on + strand at 357.531 kb on - strand at 357.531 kb on - strand at 357.564 kb on - strand at 357.566 kb on + strand at 357.566 kb on + strand at 357.635 kb on - strand at 357.722 kb on + strand at 357.781 kb on + strand, within LU632_RS01860 at 357.781 kb on + strand, within LU632_RS01860 at 357.782 kb on - strand, within LU632_RS01860 at 357.806 kb on - strand, within LU632_RS01860 at 357.874 kb on + strand, within LU632_RS01860 at 357.875 kb on - strand, within LU632_RS01860 at 357.938 kb on - strand, within LU632_RS01860 at 358.000 kb on + strand, within LU632_RS01860 at 358.000 kb on + strand, within LU632_RS01860
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 356,042 + LU632_RS01845 0.37 +1.5 356,043 - LU632_RS01845 0.37 -0.5 356,070 + LU632_RS01845 0.39 +0.1 356,070 + LU632_RS01845 0.39 +0.0 356,071 - LU632_RS01845 0.39 +0.5 356,091 + LU632_RS01845 0.41 +0.2 356,521 - LU632_RS01845 0.74 -0.1 356,521 - LU632_RS01845 0.74 -0.1 356,567 + LU632_RS01845 0.78 -1.1 356,567 + LU632_RS01845 0.78 -0.3 356,568 - LU632_RS01845 0.78 +0.2 356,568 - LU632_RS01845 0.78 -0.7 356,569 + LU632_RS01845 0.78 +0.2 356,569 + LU632_RS01845 0.78 -0.5 356,569 + LU632_RS01845 0.78 -0.1 356,569 + LU632_RS01845 0.78 +0.1 356,569 + LU632_RS01845 0.78 +0.3 356,569 + LU632_RS01845 0.78 +0.2 356,569 + LU632_RS01845 0.78 +0.3 356,570 - LU632_RS01845 0.78 -0.6 356,570 - LU632_RS01845 0.78 -0.0 356,570 - LU632_RS01845 0.78 +0.7 356,570 - LU632_RS01845 0.78 +1.2 356,570 - LU632_RS01845 0.78 +0.1 356,570 - LU632_RS01845 0.78 -0.1 356,570 - LU632_RS01845 0.78 +0.4 356,570 - LU632_RS01845 0.78 +1.2 356,573 + LU632_RS01845 0.78 +0.1 356,700 - LU632_RS01845 0.88 -0.2 356,736 + -0.6 356,841 + +0.8 356,841 + +0.9 356,842 - +0.9 356,842 - -0.4 356,842 - +0.4 356,902 - -0.5 356,962 + +1.4 356,963 - +0.4 357,040 + LU632_RS01850 0.46 -0.2 357,041 - LU632_RS01850 0.47 -0.2 357,041 - LU632_RS01850 0.47 -1.1 357,041 - LU632_RS01850 0.47 -0.0 357,041 - LU632_RS01850 0.47 -0.5 357,041 - LU632_RS01850 0.47 +0.3 357,042 + LU632_RS01850 0.47 -0.8 357,042 + LU632_RS01850 0.47 +0.2 357,042 + LU632_RS01850 0.47 +0.2 357,042 + LU632_RS01850 0.47 -2.5 357,042 + LU632_RS01850 0.47 -0.8 357,042 + LU632_RS01850 0.47 -0.0 357,043 - LU632_RS01850 0.48 -0.3 357,043 - LU632_RS01850 0.48 -0.0 357,043 - LU632_RS01850 0.48 -1.1 357,118 + -1.8 357,118 + +0.2 357,119 - -0.3 357,119 - -0.8 357,119 - +0.7 357,119 - +0.2 357,119 - -0.1 357,119 - -1.6 357,121 - +0.1 357,121 - -0.6 357,121 - +1.2 357,140 - -0.5 357,229 + aspA LU632_RS01855 0.45 +1.2 357,242 - aspA LU632_RS01855 0.51 +0.5 357,280 + aspA LU632_RS01855 0.67 +0.1 357,281 - aspA LU632_RS01855 0.67 -0.5 357,291 + aspA LU632_RS01855 0.72 -0.2 357,295 - aspA LU632_RS01855 0.73 +0.5 357,295 - aspA LU632_RS01855 0.73 -2.0 357,467 - +1.2 357,485 + +0.3 357,486 - +0.5 357,508 + +0.1 357,531 - +0.4 357,531 - +0.4 357,564 - +0.3 357,566 + -0.1 357,566 + -0.5 357,635 - -0.2 357,722 + -0.2 357,781 + LU632_RS01860 0.17 -0.3 357,781 + LU632_RS01860 0.17 -0.8 357,782 - LU632_RS01860 0.17 -1.0 357,806 - LU632_RS01860 0.22 -0.0 357,874 + LU632_RS01860 0.37 +1.1 357,875 - LU632_RS01860 0.37 -1.8 357,938 - LU632_RS01860 0.51 -0.6 358,000 + LU632_RS01860 0.65 +0.9 358,000 + LU632_RS01860 0.65 -1.8
Or see this region's nucleotide sequence