Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and LU632_RS00975 are separated by 311 nucleotides LU632_RS00975 and nudC are separated by 85 nucleotides nudC and hemE are separated by 38 nucleotides
LU632_RS00970: thiC - phosphomethylpyrimidine synthase ThiC, at 209,875 to 211,809
thiC
LU632_RS00975: LU632_RS00975 - Rsd/AlgQ family anti-sigma factor, at 212,121 to 212,630
_RS00975
LU632_RS00980: nudC - NAD(+) diphosphatase, at 212,716 to 213,489
nudC
LU632_RS00985: hemE - uroporphyrinogen decarboxylase, at 213,528 to 214,595
hemE
Position (kb)
212
213 Strain fitness (log2 ratio)
-2
-1
0
1 at 211.122 kb on + strand, within thiC at 211.152 kb on + strand, within thiC at 211.164 kb on + strand, within thiC at 211.329 kb on + strand, within thiC at 211.464 kb on + strand, within thiC at 211.465 kb on - strand, within thiC at 211.465 kb on - strand, within thiC at 211.537 kb on + strand, within thiC at 211.537 kb on + strand, within thiC at 211.537 kb on + strand, within thiC at 211.583 kb on + strand, within thiC at 211.809 kb on + strand at 211.810 kb on - strand at 211.860 kb on + strand at 211.860 kb on + strand at 211.861 kb on - strand at 211.930 kb on + strand at 211.930 kb on + strand at 212.028 kb on + strand at 212.042 kb on + strand at 212.074 kb on - strand at 212.093 kb on - strand at 212.094 kb on - strand at 212.150 kb on + strand at 212.195 kb on + strand, within LU632_RS00975 at 212.195 kb on + strand, within LU632_RS00975 at 212.198 kb on + strand, within LU632_RS00975 at 212.199 kb on - strand, within LU632_RS00975 at 212.272 kb on + strand, within LU632_RS00975 at 212.273 kb on - strand, within LU632_RS00975 at 212.313 kb on + strand, within LU632_RS00975 at 212.313 kb on + strand, within LU632_RS00975 at 212.314 kb on - strand, within LU632_RS00975 at 212.353 kb on + strand, within LU632_RS00975 at 212.403 kb on - strand, within LU632_RS00975 at 212.414 kb on - strand, within LU632_RS00975 at 212.441 kb on - strand, within LU632_RS00975 at 212.466 kb on - strand, within LU632_RS00975 at 212.530 kb on + strand, within LU632_RS00975 at 212.530 kb on + strand, within LU632_RS00975 at 212.533 kb on + strand, within LU632_RS00975 at 212.533 kb on + strand, within LU632_RS00975 at 212.681 kb on - strand at 212.715 kb on + strand at 212.716 kb on - strand at 212.716 kb on - strand at 212.738 kb on + strand at 212.899 kb on - strand, within nudC at 213.083 kb on - strand, within nudC at 213.108 kb on + strand, within nudC at 213.108 kb on + strand, within nudC at 213.109 kb on - strand, within nudC at 213.117 kb on + strand, within nudC at 213.154 kb on - strand, within nudC at 213.175 kb on + strand, within nudC at 213.175 kb on + strand, within nudC at 213.175 kb on + strand, within nudC at 213.175 kb on + strand, within nudC at 213.175 kb on + strand, within nudC at 213.176 kb on - strand, within nudC at 213.176 kb on - strand, within nudC at 213.176 kb on - strand, within nudC at 213.277 kb on + strand, within nudC at 213.277 kb on + strand, within nudC at 213.277 kb on + strand, within nudC at 213.278 kb on - strand, within nudC at 213.337 kb on + strand, within nudC at 213.496 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 211,122 + thiC LU632_RS00970 0.64 +0.0 211,152 + thiC LU632_RS00970 0.66 +0.2 211,164 + thiC LU632_RS00970 0.67 -0.6 211,329 + thiC LU632_RS00970 0.75 -0.3 211,464 + thiC LU632_RS00970 0.82 +0.5 211,465 - thiC LU632_RS00970 0.82 -1.8 211,465 - thiC LU632_RS00970 0.82 -0.3 211,537 + thiC LU632_RS00970 0.86 +0.8 211,537 + thiC LU632_RS00970 0.86 +0.1 211,537 + thiC LU632_RS00970 0.86 +0.6 211,583 + thiC LU632_RS00970 0.88 -0.1 211,809 + -0.3 211,810 - +0.0 211,860 + -0.8 211,860 + -0.5 211,861 - +0.0 211,930 + +0.2 211,930 + -0.0 212,028 + +0.0 212,042 + +0.1 212,074 - -0.2 212,093 - -0.1 212,094 - +0.8 212,150 + +0.4 212,195 + LU632_RS00975 0.15 +1.7 212,195 + LU632_RS00975 0.15 -1.1 212,198 + LU632_RS00975 0.15 +0.2 212,199 - LU632_RS00975 0.15 +0.6 212,272 + LU632_RS00975 0.30 -0.2 212,273 - LU632_RS00975 0.30 -0.0 212,313 + LU632_RS00975 0.38 -0.8 212,313 + LU632_RS00975 0.38 -1.6 212,314 - LU632_RS00975 0.38 -0.4 212,353 + LU632_RS00975 0.45 -0.7 212,403 - LU632_RS00975 0.55 -1.1 212,414 - LU632_RS00975 0.57 -0.2 212,441 - LU632_RS00975 0.63 -0.8 212,466 - LU632_RS00975 0.68 -0.1 212,530 + LU632_RS00975 0.80 -0.8 212,530 + LU632_RS00975 0.80 -1.4 212,533 + LU632_RS00975 0.81 +0.7 212,533 + LU632_RS00975 0.81 -0.9 212,681 - -0.5 212,715 + -0.6 212,716 - -0.1 212,716 - +0.2 212,738 + -0.7 212,899 - nudC LU632_RS00980 0.24 -0.4 213,083 - nudC LU632_RS00980 0.47 -1.8 213,108 + nudC LU632_RS00980 0.51 -0.3 213,108 + nudC LU632_RS00980 0.51 -0.9 213,109 - nudC LU632_RS00980 0.51 +0.3 213,117 + nudC LU632_RS00980 0.52 +0.7 213,154 - nudC LU632_RS00980 0.57 +0.9 213,175 + nudC LU632_RS00980 0.59 -0.2 213,175 + nudC LU632_RS00980 0.59 -1.8 213,175 + nudC LU632_RS00980 0.59 +0.1 213,175 + nudC LU632_RS00980 0.59 -1.8 213,175 + nudC LU632_RS00980 0.59 -1.8 213,176 - nudC LU632_RS00980 0.59 -1.1 213,176 - nudC LU632_RS00980 0.59 -0.7 213,176 - nudC LU632_RS00980 0.59 -0.1 213,277 + nudC LU632_RS00980 0.72 -0.7 213,277 + nudC LU632_RS00980 0.72 -0.7 213,277 + nudC LU632_RS00980 0.72 -0.1 213,278 - nudC LU632_RS00980 0.73 -0.4 213,337 + nudC LU632_RS00980 0.80 +0.5 213,496 + -0.8
Or see this region's nucleotide sequence