Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctE and LU632_RS00520 are separated by 34 nucleotides LU632_RS00520 and LU632_RS00525 are separated by 70 nucleotides
LU632_RS00515: sctE - type III secretion system translocon subunit SctE, at 104,470 to 106,341
sctE
LU632_RS00520: LU632_RS00520 - hypothetical protein, at 106,376 to 107,581
_RS00520
LU632_RS00525: LU632_RS00525 - IpaD/SipD/SspD family type III secretion system needle tip protein, at 107,652 to 108,830
_RS00525
Position (kb) 106
107
108 Strain fitness (log2 ratio) -2
-1
0
1
2 at 105.381 kb on + strand, within sctE at 105.591 kb on + strand, within sctE at 105.727 kb on - strand, within sctE at 105.812 kb on + strand, within sctE at 105.812 kb on + strand, within sctE at 105.813 kb on - strand, within sctE at 105.845 kb on + strand, within sctE at 105.859 kb on + strand, within sctE at 105.952 kb on - strand, within sctE at 106.247 kb on + strand at 106.414 kb on + strand at 106.683 kb on + strand, within LU632_RS00520 at 106.684 kb on - strand, within LU632_RS00520 at 106.684 kb on - strand, within LU632_RS00520 at 106.738 kb on + strand, within LU632_RS00520 at 106.752 kb on + strand, within LU632_RS00520 at 106.758 kb on + strand, within LU632_RS00520 at 106.774 kb on + strand, within LU632_RS00520 at 106.793 kb on + strand, within LU632_RS00520 at 106.793 kb on + strand, within LU632_RS00520 at 106.794 kb on - strand, within LU632_RS00520 at 106.794 kb on - strand, within LU632_RS00520 at 106.862 kb on - strand, within LU632_RS00520 at 106.927 kb on + strand, within LU632_RS00520 at 106.927 kb on + strand, within LU632_RS00520 at 106.928 kb on - strand, within LU632_RS00520 at 106.931 kb on - strand, within LU632_RS00520 at 106.992 kb on + strand, within LU632_RS00520 at 106.992 kb on + strand, within LU632_RS00520 at 107.011 kb on + strand, within LU632_RS00520 at 107.011 kb on + strand, within LU632_RS00520 at 107.012 kb on - strand, within LU632_RS00520 at 107.060 kb on - strand, within LU632_RS00520 at 107.062 kb on + strand, within LU632_RS00520 at 107.065 kb on + strand, within LU632_RS00520 at 107.065 kb on + strand, within LU632_RS00520 at 107.134 kb on + strand, within LU632_RS00520 at 107.236 kb on - strand, within LU632_RS00520 at 108.250 kb on - strand, within LU632_RS00525 at 108.373 kb on + strand, within LU632_RS00525 at 108.453 kb on - strand, within LU632_RS00525 at 108.529 kb on + strand, within LU632_RS00525 at 108.549 kb on + strand, within LU632_RS00525
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 105,381 + sctE LU632_RS00515 0.49 +0.3 105,591 + sctE LU632_RS00515 0.60 +0.4 105,727 - sctE LU632_RS00515 0.67 -0.0 105,812 + sctE LU632_RS00515 0.72 +0.2 105,812 + sctE LU632_RS00515 0.72 +0.4 105,813 - sctE LU632_RS00515 0.72 +0.1 105,845 + sctE LU632_RS00515 0.73 -0.3 105,859 + sctE LU632_RS00515 0.74 -0.3 105,952 - sctE LU632_RS00515 0.79 -0.1 106,247 + -2.4 106,414 + +0.1 106,683 + LU632_RS00520 0.25 +0.5 106,684 - LU632_RS00520 0.26 +0.0 106,684 - LU632_RS00520 0.26 +0.1 106,738 + LU632_RS00520 0.30 +0.1 106,752 + LU632_RS00520 0.31 -0.1 106,758 + LU632_RS00520 0.32 -0.0 106,774 + LU632_RS00520 0.33 +1.3 106,793 + LU632_RS00520 0.35 -0.1 106,793 + LU632_RS00520 0.35 -0.8 106,794 - LU632_RS00520 0.35 -0.4 106,794 - LU632_RS00520 0.35 -0.7 106,862 - LU632_RS00520 0.40 +0.2 106,927 + LU632_RS00520 0.46 +0.2 106,927 + LU632_RS00520 0.46 +1.5 106,928 - LU632_RS00520 0.46 -0.9 106,931 - LU632_RS00520 0.46 +0.7 106,992 + LU632_RS00520 0.51 +2.3 106,992 + LU632_RS00520 0.51 +0.6 107,011 + LU632_RS00520 0.53 -0.5 107,011 + LU632_RS00520 0.53 -0.2 107,012 - LU632_RS00520 0.53 -0.9 107,060 - LU632_RS00520 0.57 -0.1 107,062 + LU632_RS00520 0.57 +0.2 107,065 + LU632_RS00520 0.57 -0.9 107,065 + LU632_RS00520 0.57 -0.2 107,134 + LU632_RS00520 0.63 -0.6 107,236 - LU632_RS00520 0.71 -0.1 108,250 - LU632_RS00525 0.51 -1.4 108,373 + LU632_RS00525 0.61 +0.0 108,453 - LU632_RS00525 0.68 -0.5 108,529 + LU632_RS00525 0.74 -1.1 108,549 + LU632_RS00525 0.76 -0.3
Or see this region's nucleotide sequence