Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slmA and pyrE are separated by 30 nucleotides pyrE and rph are separated by 54 nucleotides rph and LU632_RS22025 are separated by 56 nucleotides LU632_RS22025 and LU632_RS22030 are separated by 134 nucleotides
LU632_RS22010: slmA - nucleoid occlusion factor SlmA, at 4,202,988 to 4,203,584
slmA
LU632_RS22015: pyrE - orotate phosphoribosyltransferase, at 4,203,615 to 4,204,256
pyrE
LU632_RS22020: rph - ribonuclease PH, at 4,204,311 to 4,205,027
rph
LU632_RS22025: LU632_RS22025 - hypothetical protein, at 4,205,084 to 4,205,326
_RS22025
LU632_RS22030: LU632_RS22030 - ABC transporter permease, at 4,205,461 to 4,206,552
_RS22030
Position (kb) 4204
4205
4206 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4203.371 kb on - strand, within slmA at 4203.896 kb on + strand, within pyrE at 4203.897 kb on - strand, within pyrE at 4203.897 kb on - strand, within pyrE at 4203.899 kb on + strand, within pyrE at 4203.899 kb on + strand, within pyrE at 4203.973 kb on + strand, within pyrE at 4203.974 kb on - strand, within pyrE at 4203.979 kb on - strand, within pyrE at 4203.979 kb on - strand, within pyrE at 4204.035 kb on - strand, within pyrE at 4204.042 kb on + strand, within pyrE at 4204.043 kb on - strand, within pyrE at 4204.043 kb on - strand, within pyrE at 4204.056 kb on + strand, within pyrE at 4204.057 kb on - strand, within pyrE at 4204.312 kb on + strand at 4204.312 kb on + strand at 4204.578 kb on + strand, within rph at 4204.579 kb on - strand, within rph at 4204.579 kb on - strand, within rph at 4204.583 kb on + strand, within rph at 4204.584 kb on - strand, within rph at 4204.584 kb on - strand, within rph at 4204.694 kb on + strand, within rph at 4204.694 kb on + strand, within rph at 4204.694 kb on + strand, within rph at 4204.695 kb on - strand, within rph at 4204.835 kb on + strand, within rph at 4204.835 kb on + strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.841 kb on - strand, within rph at 4204.969 kb on + strand at 4205.054 kb on + strand at 4205.070 kb on + strand at 4205.101 kb on + strand at 4205.102 kb on - strand at 4205.104 kb on + strand at 4205.104 kb on + strand at 4205.104 kb on + strand at 4205.160 kb on + strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.253 kb on - strand, within LU632_RS22025 at 4205.327 kb on - strand at 4205.373 kb on - strand at 4205.396 kb on + strand at 4205.397 kb on - strand at 4205.397 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.425 kb on - strand at 4205.495 kb on - strand at 4205.495 kb on - strand at 4205.496 kb on + strand at 4205.497 kb on - strand at 4205.612 kb on - strand, within LU632_RS22030 at 4205.662 kb on - strand, within LU632_RS22030 at 4205.674 kb on + strand, within LU632_RS22030 at 4205.675 kb on - strand, within LU632_RS22030 at 4205.691 kb on + strand, within LU632_RS22030 at 4205.723 kb on - strand, within LU632_RS22030 at 4205.754 kb on + strand, within LU632_RS22030 at 4205.780 kb on + strand, within LU632_RS22030 at 4205.794 kb on + strand, within LU632_RS22030 at 4205.795 kb on - strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.797 kb on - strand, within LU632_RS22030 at 4205.859 kb on + strand, within LU632_RS22030 at 4205.876 kb on - strand, within LU632_RS22030 at 4205.891 kb on + strand, within LU632_RS22030 at 4205.892 kb on - strand, within LU632_RS22030
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 4,203,371 - slmA LU632_RS22010 0.64 +0.1 4,203,896 + pyrE LU632_RS22015 0.44 -4.7 4,203,897 - pyrE LU632_RS22015 0.44 +1.1 4,203,897 - pyrE LU632_RS22015 0.44 -1.9 4,203,899 + pyrE LU632_RS22015 0.44 -2.4 4,203,899 + pyrE LU632_RS22015 0.44 -2.5 4,203,973 + pyrE LU632_RS22015 0.56 +0.1 4,203,974 - pyrE LU632_RS22015 0.56 -1.7 4,203,979 - pyrE LU632_RS22015 0.57 -2.2 4,203,979 - pyrE LU632_RS22015 0.57 -1.5 4,204,035 - pyrE LU632_RS22015 0.65 -2.7 4,204,042 + pyrE LU632_RS22015 0.67 -1.5 4,204,043 - pyrE LU632_RS22015 0.67 -4.5 4,204,043 - pyrE LU632_RS22015 0.67 -3.4 4,204,056 + pyrE LU632_RS22015 0.69 -1.7 4,204,057 - pyrE LU632_RS22015 0.69 -1.9 4,204,312 + -2.7 4,204,312 + -1.7 4,204,578 + rph LU632_RS22020 0.37 -0.9 4,204,579 - rph LU632_RS22020 0.37 +0.2 4,204,579 - rph LU632_RS22020 0.37 +0.1 4,204,583 + rph LU632_RS22020 0.38 -1.3 4,204,584 - rph LU632_RS22020 0.38 +0.6 4,204,584 - rph LU632_RS22020 0.38 -0.5 4,204,694 + rph LU632_RS22020 0.53 +0.3 4,204,694 + rph LU632_RS22020 0.53 -3.1 4,204,694 + rph LU632_RS22020 0.53 +0.5 4,204,695 - rph LU632_RS22020 0.54 +0.4 4,204,835 + rph LU632_RS22020 0.73 +0.5 4,204,835 + rph LU632_RS22020 0.73 -2.1 4,204,836 - rph LU632_RS22020 0.73 -0.5 4,204,836 - rph LU632_RS22020 0.73 +0.2 4,204,836 - rph LU632_RS22020 0.73 +0.8 4,204,836 - rph LU632_RS22020 0.73 +1.3 4,204,836 - rph LU632_RS22020 0.73 +1.1 4,204,836 - rph LU632_RS22020 0.73 -0.9 4,204,841 - rph LU632_RS22020 0.74 +0.5 4,204,969 + -2.2 4,205,054 + -0.6 4,205,070 + +0.1 4,205,101 + -0.4 4,205,102 - -0.6 4,205,104 + -0.3 4,205,104 + +2.3 4,205,104 + +0.1 4,205,160 + LU632_RS22025 0.31 +0.1 4,205,161 - LU632_RS22025 0.32 -0.9 4,205,161 - LU632_RS22025 0.32 -1.0 4,205,161 - LU632_RS22025 0.32 -1.7 4,205,253 - LU632_RS22025 0.70 +0.2 4,205,327 - +0.5 4,205,373 - +0.9 4,205,396 + +0.3 4,205,397 - -0.1 4,205,397 - +1.5 4,205,399 - +0.2 4,205,399 - -0.6 4,205,399 - +0.1 4,205,399 - -1.2 4,205,425 - +0.8 4,205,495 - -0.3 4,205,495 - +0.9 4,205,496 + +0.4 4,205,497 - +0.4 4,205,612 - LU632_RS22030 0.14 +1.1 4,205,662 - LU632_RS22030 0.18 +0.0 4,205,674 + LU632_RS22030 0.20 +0.3 4,205,675 - LU632_RS22030 0.20 -0.7 4,205,691 + LU632_RS22030 0.21 +0.2 4,205,723 - LU632_RS22030 0.24 +0.2 4,205,754 + LU632_RS22030 0.27 +0.0 4,205,780 + LU632_RS22030 0.29 -0.9 4,205,794 + LU632_RS22030 0.30 -0.6 4,205,795 - LU632_RS22030 0.31 +0.4 4,205,796 + LU632_RS22030 0.31 +0.6 4,205,796 + LU632_RS22030 0.31 +0.1 4,205,796 + LU632_RS22030 0.31 +0.6 4,205,797 - LU632_RS22030 0.31 -0.1 4,205,859 + LU632_RS22030 0.36 +1.5 4,205,876 - LU632_RS22030 0.38 +0.4 4,205,891 + LU632_RS22030 0.39 +0.6 4,205,892 - LU632_RS22030 0.39 +0.2
Or see this region's nucleotide sequence