Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argH and LU632_RS19605 are separated by 84 nucleotides LU632_RS19605 and argB are separated by 19 nucleotides argB and argC are separated by 15 nucleotides
LU632_RS19600: argH - argininosuccinate lyase, at 3,716,815 to 3,718,188
argH
LU632_RS19605: LU632_RS19605 - argininosuccinate synthase, at 3,718,273 to 3,719,487
_RS19605
LU632_RS19610: argB - acetylglutamate kinase, at 3,719,507 to 3,720,283
argB
LU632_RS19615: argC - N-acetyl-gamma-glutamyl-phosphate reductase, at 3,720,299 to 3,721,303
argC
Position (kb)
3718
3719
3720 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3717.317 kb on - strand, within argH at 3717.317 kb on - strand, within argH at 3717.317 kb on - strand, within argH at 3717.408 kb on + strand, within argH at 3717.628 kb on - strand, within argH at 3717.675 kb on - strand, within argH at 3717.729 kb on - strand, within argH at 3717.729 kb on - strand, within argH at 3717.785 kb on - strand, within argH at 3717.876 kb on + strand, within argH at 3717.880 kb on + strand, within argH at 3717.881 kb on - strand, within argH at 3717.930 kb on - strand, within argH at 3717.930 kb on - strand, within argH at 3717.930 kb on - strand, within argH at 3717.930 kb on - strand, within argH at 3717.990 kb on + strand, within argH at 3718.075 kb on + strand at 3718.075 kb on + strand at 3718.077 kb on + strand at 3718.078 kb on - strand at 3718.078 kb on - strand at 3718.078 kb on - strand at 3718.087 kb on - strand at 3718.215 kb on + strand at 3718.218 kb on + strand at 3718.225 kb on - strand at 3718.352 kb on + strand at 3718.402 kb on - strand, within LU632_RS19605 at 3718.535 kb on + strand, within LU632_RS19605 at 3718.536 kb on - strand, within LU632_RS19605 at 3718.544 kb on + strand, within LU632_RS19605 at 3718.544 kb on + strand, within LU632_RS19605 at 3718.545 kb on - strand, within LU632_RS19605 at 3718.545 kb on - strand, within LU632_RS19605 at 3718.545 kb on - strand, within LU632_RS19605 at 3718.545 kb on - strand, within LU632_RS19605 at 3719.079 kb on - strand, within LU632_RS19605 at 3719.196 kb on + strand, within LU632_RS19605 at 3719.213 kb on + strand, within LU632_RS19605 at 3719.214 kb on - strand, within LU632_RS19605 at 3719.218 kb on - strand, within LU632_RS19605 at 3719.226 kb on - strand, within LU632_RS19605 at 3719.258 kb on + strand, within LU632_RS19605 at 3719.399 kb on + strand at 3719.555 kb on + strand at 3719.630 kb on + strand, within argB at 3719.644 kb on + strand, within argB at 3719.646 kb on - strand, within argB at 3719.656 kb on + strand, within argB at 3719.892 kb on - strand, within argB at 3719.979 kb on - strand, within argB at 3720.028 kb on + strand, within argB at 3720.269 kb on + strand at 3720.269 kb on + strand at 3720.270 kb on - strand at 3720.270 kb on - strand at 3720.300 kb on + strand at 3720.337 kb on + strand at 3720.338 kb on - strand at 3720.340 kb on + strand at 3720.340 kb on + strand at 3720.341 kb on - strand at 3720.341 kb on - strand at 3720.486 kb on + strand, within argC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,717,317 - argH LU632_RS19600 0.37 -0.6 3,717,317 - argH LU632_RS19600 0.37 -1.6 3,717,317 - argH LU632_RS19600 0.37 -3.6 3,717,408 + argH LU632_RS19600 0.43 -3.5 3,717,628 - argH LU632_RS19600 0.59 -1.2 3,717,675 - argH LU632_RS19600 0.63 -2.0 3,717,729 - argH LU632_RS19600 0.67 -2.5 3,717,729 - argH LU632_RS19600 0.67 -1.4 3,717,785 - argH LU632_RS19600 0.71 -1.7 3,717,876 + argH LU632_RS19600 0.77 -3.3 3,717,880 + argH LU632_RS19600 0.78 -1.0 3,717,881 - argH LU632_RS19600 0.78 -1.4 3,717,930 - argH LU632_RS19600 0.81 -2.5 3,717,930 - argH LU632_RS19600 0.81 -3.5 3,717,930 - argH LU632_RS19600 0.81 -2.1 3,717,930 - argH LU632_RS19600 0.81 -2.8 3,717,990 + argH LU632_RS19600 0.86 -2.9 3,718,075 + -2.6 3,718,075 + -2.9 3,718,077 + -3.1 3,718,078 - -2.9 3,718,078 - -3.0 3,718,078 - -3.0 3,718,087 - -1.0 3,718,215 + -3.8 3,718,218 + -2.6 3,718,225 - -0.4 3,718,352 + -1.6 3,718,402 - LU632_RS19605 0.11 -2.4 3,718,535 + LU632_RS19605 0.22 -3.3 3,718,536 - LU632_RS19605 0.22 -3.1 3,718,544 + LU632_RS19605 0.22 -1.8 3,718,544 + LU632_RS19605 0.22 -2.7 3,718,545 - LU632_RS19605 0.22 -2.1 3,718,545 - LU632_RS19605 0.22 -2.5 3,718,545 - LU632_RS19605 0.22 -2.2 3,718,545 - LU632_RS19605 0.22 -1.8 3,719,079 - LU632_RS19605 0.66 -1.0 3,719,196 + LU632_RS19605 0.76 -2.5 3,719,213 + LU632_RS19605 0.77 -3.2 3,719,214 - LU632_RS19605 0.77 -2.3 3,719,218 - LU632_RS19605 0.78 -2.7 3,719,226 - LU632_RS19605 0.78 -2.4 3,719,258 + LU632_RS19605 0.81 -3.9 3,719,399 + -2.4 3,719,555 + -1.7 3,719,630 + argB LU632_RS19610 0.16 -1.8 3,719,644 + argB LU632_RS19610 0.18 -3.2 3,719,646 - argB LU632_RS19610 0.18 -2.6 3,719,656 + argB LU632_RS19610 0.19 -2.5 3,719,892 - argB LU632_RS19610 0.50 -0.6 3,719,979 - argB LU632_RS19610 0.61 -2.2 3,720,028 + argB LU632_RS19610 0.67 -2.9 3,720,269 + -2.7 3,720,269 + -2.1 3,720,270 - -3.4 3,720,270 - -1.7 3,720,300 + -0.6 3,720,337 + -1.9 3,720,338 - -1.8 3,720,340 + -2.8 3,720,340 + +0.4 3,720,341 - -2.6 3,720,341 - -2.5 3,720,486 + argC LU632_RS19615 0.19 -0.0
Or see this region's nucleotide sequence