Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS18015 and LU632_RS18020 are separated by 158 nucleotides LU632_RS18020 and LU632_RS18025 overlap by 79 nucleotides LU632_RS18025 and LU632_RS18030 are separated by 302 nucleotides LU632_RS18030 and LU632_RS18035 are separated by 15 nucleotides
LU632_RS18015: LU632_RS18015 - hypothetical protein, at 3,405,070 to 3,405,468
_RS18015
LU632_RS18020: LU632_RS18020 - hypothetical protein, at 3,405,627 to 3,406,508
_RS18020
LU632_RS18025: LU632_RS18025 - IS1 family transposase, at 3,406,430 to 3,406,997
_RS18025
LU632_RS18030: LU632_RS18030 - hypothetical protein, at 3,407,300 to 3,407,605
_RS18030
LU632_RS18035: LU632_RS18035 - transposase, at 3,407,621 to 3,407,794
_RS18035
Position (kb)
3406
3407 Strain fitness (log2 ratio)
-1
0
1 at 3405.452 kb on - strand at 3405.456 kb on + strand at 3405.507 kb on - strand at 3405.507 kb on - strand at 3405.537 kb on - strand at 3405.656 kb on + strand at 3405.906 kb on - strand, within LU632_RS18020 at 3407.048 kb on - strand at 3407.146 kb on + strand at 3407.156 kb on - strand at 3407.176 kb on - strand at 3407.183 kb on - strand at 3407.185 kb on + strand at 3407.202 kb on + strand at 3407.263 kb on + strand at 3407.299 kb on + strand at 3407.300 kb on - strand at 3407.499 kb on + strand, within LU632_RS18030 at 3407.603 kb on + strand at 3407.606 kb on + strand at 3407.606 kb on + strand at 3407.606 kb on + strand at 3407.607 kb on - strand at 3407.608 kb on + strand at 3407.609 kb on - strand at 3407.617 kb on + strand at 3407.617 kb on + strand at 3407.853 kb on + strand at 3407.863 kb on - strand at 3407.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,405,452 - -0.5 3,405,456 + +0.9 3,405,507 - +0.0 3,405,507 - +1.1 3,405,537 - -0.4 3,405,656 + +1.3 3,405,906 - LU632_RS18020 0.32 -0.2 3,407,048 - -1.1 3,407,146 + +0.3 3,407,156 - -0.4 3,407,176 - -0.2 3,407,183 - -0.3 3,407,185 + +0.4 3,407,202 + +1.1 3,407,263 + -0.2 3,407,299 + -0.3 3,407,300 - -1.0 3,407,499 + LU632_RS18030 0.65 +0.0 3,407,603 + +0.2 3,407,606 + -0.1 3,407,606 + -0.3 3,407,606 + -0.0 3,407,607 - -0.1 3,407,608 + -0.5 3,407,609 - -0.0 3,407,617 + +0.9 3,407,617 + +0.3 3,407,853 + +0.3 3,407,863 - -0.2 3,407,943 - -0.6
Or see this region's nucleotide sequence