Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS16270 and LU632_RS16275 are separated by 7 nucleotides LU632_RS16275 and LU632_RS16280 are separated by 23 nucleotides LU632_RS16280 and LU632_RS16285 are separated by 43 nucleotides LU632_RS16285 and LU632_RS16290 are separated by 7 nucleotides LU632_RS16290 and LU632_RS16295 are separated by 38 nucleotides LU632_RS16295 and ubiF are separated by 101 nucleotides ubiF and miaB are separated by 184 nucleotides
LU632_RS16270: LU632_RS16270 - tRNA-Met, at 3,081,901 to 3,081,977
_RS16270
LU632_RS16275: LU632_RS16275 - tRNA-Leu, at 3,081,985 to 3,082,069
_RS16275
LU632_RS16280: LU632_RS16280 - tRNA-Gln, at 3,082,093 to 3,082,167
_RS16280
LU632_RS16285: LU632_RS16285 - tRNA-Met, at 3,082,211 to 3,082,287
_RS16285
LU632_RS16290: LU632_RS16290 - tRNA-Gln, at 3,082,295 to 3,082,369
_RS16290
LU632_RS16295: LU632_RS16295 - tRNA-Gln, at 3,082,408 to 3,082,482
_RS16295
LU632_RS16300: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 3,082,584 to 3,083,774
ubiF
LU632_RS16305: miaB - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 3,083,959 to 3,085,383
miaB
Position (kb)
3082
3083
3084 Strain fitness (log2 ratio)
-1
0
1
2 at 3081.592 kb on + strand at 3081.593 kb on - strand at 3081.593 kb on - strand at 3081.593 kb on - strand at 3081.593 kb on - strand at 3081.612 kb on - strand at 3081.612 kb on - strand at 3081.613 kb on + strand at 3081.613 kb on + strand at 3081.613 kb on + strand at 3081.681 kb on + strand at 3081.756 kb on - strand at 3081.785 kb on + strand at 3082.189 kb on + strand at 3082.190 kb on - strand at 3082.219 kb on - strand, within LU632_RS16285 at 3082.219 kb on - strand, within LU632_RS16285 at 3082.374 kb on + strand at 3082.374 kb on + strand at 3082.374 kb on + strand at 3082.375 kb on - strand at 3082.402 kb on + strand at 3082.403 kb on - strand at 3082.403 kb on - strand at 3082.406 kb on - strand at 3082.502 kb on + strand at 3082.503 kb on - strand at 3082.503 kb on - strand at 3082.524 kb on + strand at 3082.524 kb on + strand at 3082.526 kb on + strand at 3082.527 kb on - strand at 3082.552 kb on + strand at 3082.552 kb on + strand at 3082.555 kb on + strand at 3083.831 kb on - strand at 3083.884 kb on + strand at 3083.884 kb on + strand at 3083.884 kb on + strand at 3083.884 kb on + strand at 3083.885 kb on - strand at 3083.885 kb on - strand at 3083.913 kb on - strand at 3083.946 kb on + strand at 3084.055 kb on - strand at 3084.099 kb on + strand at 3084.100 kb on - strand at 3084.100 kb on - strand at 3084.190 kb on - strand, within miaB at 3084.263 kb on + strand, within miaB at 3084.433 kb on - strand, within miaB at 3084.437 kb on - strand, within miaB at 3084.441 kb on + strand, within miaB at 3084.441 kb on + strand, within miaB at 3084.590 kb on - strand, within miaB at 3084.590 kb on - strand, within miaB at 3084.591 kb on + strand, within miaB at 3084.602 kb on + strand, within miaB at 3084.602 kb on + strand, within miaB at 3084.603 kb on - strand, within miaB
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,081,592 + +0.7 3,081,593 - -0.4 3,081,593 - +0.2 3,081,593 - -0.1 3,081,593 - +0.2 3,081,612 - -0.1 3,081,612 - -0.3 3,081,613 + +0.3 3,081,613 + -1.2 3,081,613 + +0.9 3,081,681 + +0.2 3,081,756 - +0.4 3,081,785 + -0.2 3,082,189 + -0.5 3,082,190 - -0.2 3,082,219 - LU632_RS16285 0.10 -0.6 3,082,219 - LU632_RS16285 0.10 +0.3 3,082,374 + -0.4 3,082,374 + -0.2 3,082,374 + +0.0 3,082,375 - +0.6 3,082,402 + -0.4 3,082,403 - +0.4 3,082,403 - +0.4 3,082,406 - -1.0 3,082,502 + -0.1 3,082,503 - -0.2 3,082,503 - +0.0 3,082,524 + -0.4 3,082,524 + +0.3 3,082,526 + -0.3 3,082,527 - -0.1 3,082,552 + +0.0 3,082,552 + +0.8 3,082,555 + -0.5 3,083,831 - +0.1 3,083,884 + -0.5 3,083,884 + +0.2 3,083,884 + -0.2 3,083,884 + -0.4 3,083,885 - +0.3 3,083,885 - +0.1 3,083,913 - +0.2 3,083,946 + -0.6 3,084,055 - -0.8 3,084,099 + -0.7 3,084,100 - -0.5 3,084,100 - -1.1 3,084,190 - miaB LU632_RS16305 0.16 -0.6 3,084,263 + miaB LU632_RS16305 0.21 +0.3 3,084,433 - miaB LU632_RS16305 0.33 +1.8 3,084,437 - miaB LU632_RS16305 0.34 -0.4 3,084,441 + miaB LU632_RS16305 0.34 -0.2 3,084,441 + miaB LU632_RS16305 0.34 -0.8 3,084,590 - miaB LU632_RS16305 0.44 -0.2 3,084,590 - miaB LU632_RS16305 0.44 +0.8 3,084,591 + miaB LU632_RS16305 0.44 -0.5 3,084,602 + miaB LU632_RS16305 0.45 +0.6 3,084,602 + miaB LU632_RS16305 0.45 -0.1 3,084,603 - miaB LU632_RS16305 0.45 -1.5
Or see this region's nucleotide sequence