Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14635 and cobB are separated by 50 nucleotides cobB and lolE are separated by 50 nucleotides lolE and lolD overlap by 1 nucleotides lolD and lolC overlap by 8 nucleotides
LU632_RS14635: LU632_RS14635 - metal ABC transporter permease, at 2,783,519 to 2,784,382
_RS14635
LU632_RS14640: cobB - NAD-dependent protein deacylase, at 2,784,433 to 2,785,266
cobB
LU632_RS14645: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 2,785,317 to 2,786,561
lolE
LU632_RS14650: lolD - lipoprotein-releasing ABC transporter ATP-binding protein LolD, at 2,786,561 to 2,787,265
lolD
LU632_RS14655: lolC - lipoprotein-releasing ABC transporter permease subunit LolC, at 2,787,258 to 2,788,457
lolC
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1 at 2784.346 kb on + strand at 2784.347 kb on - strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.434 kb on + strand at 2784.435 kb on - strand at 2784.435 kb on - strand at 2784.558 kb on - strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.826 kb on - strand, within cobB at 2784.826 kb on - strand, within cobB at 2784.965 kb on - strand, within cobB at 2785.269 kb on + strand at 2785.269 kb on + strand at 2785.278 kb on + strand at 2787.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,784,346 + +0.2 2,784,347 - -2.0 2,784,416 + +1.0 2,784,416 + +0.2 2,784,416 + -0.6 2,784,416 + +1.1 2,784,434 + +1.3 2,784,435 - +0.2 2,784,435 - -0.7 2,784,558 - cobB LU632_RS14640 0.15 +0.0 2,784,825 + cobB LU632_RS14640 0.47 -0.8 2,784,825 + cobB LU632_RS14640 0.47 +0.0 2,784,825 + cobB LU632_RS14640 0.47 +0.3 2,784,825 + cobB LU632_RS14640 0.47 -0.3 2,784,826 - cobB LU632_RS14640 0.47 -0.8 2,784,826 - cobB LU632_RS14640 0.47 +0.1 2,784,965 - cobB LU632_RS14640 0.64 +0.7 2,785,269 + -1.0 2,785,269 + -0.8 2,785,278 + +0.1 2,787,266 - -2.3
Or see this region's nucleotide sequence