Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14630 and LU632_RS14635 overlap by 4 nucleotides LU632_RS14635 and cobB are separated by 50 nucleotides cobB and lolE are separated by 50 nucleotides
LU632_RS14630: LU632_RS14630 - metal ABC transporter permease, at 2,782,656 to 2,783,522
_RS14630
LU632_RS14635: LU632_RS14635 - metal ABC transporter permease, at 2,783,519 to 2,784,382
_RS14635
LU632_RS14640: cobB - NAD-dependent protein deacylase, at 2,784,433 to 2,785,266
cobB
LU632_RS14645: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 2,785,317 to 2,786,561
lolE
Position (kb)
2784
2785
2786 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2783.486 kb on + strand at 2783.486 kb on + strand at 2783.486 kb on + strand at 2783.487 kb on - strand at 2783.487 kb on - strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.488 kb on + strand at 2783.489 kb on - strand at 2783.489 kb on - strand at 2783.489 kb on - strand at 2783.489 kb on - strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.490 kb on + strand at 2783.634 kb on + strand, within LU632_RS14635 at 2783.699 kb on + strand, within LU632_RS14635 at 2783.699 kb on + strand, within LU632_RS14635 at 2783.700 kb on - strand, within LU632_RS14635 at 2783.701 kb on + strand, within LU632_RS14635 at 2783.701 kb on + strand, within LU632_RS14635 at 2783.702 kb on - strand, within LU632_RS14635 at 2784.036 kb on + strand, within LU632_RS14635 at 2784.036 kb on + strand, within LU632_RS14635 at 2784.036 kb on + strand, within LU632_RS14635 at 2784.037 kb on - strand, within LU632_RS14635 at 2784.041 kb on - strand, within LU632_RS14635 at 2784.117 kb on + strand, within LU632_RS14635 at 2784.117 kb on + strand, within LU632_RS14635 at 2784.117 kb on + strand, within LU632_RS14635 at 2784.117 kb on + strand, within LU632_RS14635 at 2784.283 kb on - strand, within LU632_RS14635 at 2784.346 kb on + strand at 2784.347 kb on - strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.416 kb on + strand at 2784.434 kb on + strand at 2784.435 kb on - strand at 2784.435 kb on - strand at 2784.558 kb on - strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.825 kb on + strand, within cobB at 2784.826 kb on - strand, within cobB at 2784.826 kb on - strand, within cobB at 2784.965 kb on - strand, within cobB at 2785.269 kb on + strand at 2785.269 kb on + strand at 2785.278 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,783,486 + -0.9 2,783,486 + +1.7 2,783,486 + +0.4 2,783,487 - +0.4 2,783,487 - +0.7 2,783,488 + +0.7 2,783,488 + +0.3 2,783,488 + -0.1 2,783,488 + -0.0 2,783,488 + -0.2 2,783,488 + +2.0 2,783,488 + +0.7 2,783,488 + +0.3 2,783,489 - +0.2 2,783,489 - +0.5 2,783,489 - -0.3 2,783,489 - +0.9 2,783,490 + -0.0 2,783,490 + -0.2 2,783,490 + -0.3 2,783,490 + -0.0 2,783,490 + +0.3 2,783,490 + +0.3 2,783,490 + -0.4 2,783,490 + -0.1 2,783,634 + LU632_RS14635 0.13 -0.6 2,783,699 + LU632_RS14635 0.21 -0.0 2,783,699 + LU632_RS14635 0.21 +0.4 2,783,700 - LU632_RS14635 0.21 -0.0 2,783,701 + LU632_RS14635 0.21 +0.6 2,783,701 + LU632_RS14635 0.21 -0.2 2,783,702 - LU632_RS14635 0.21 -0.4 2,784,036 + LU632_RS14635 0.60 -0.3 2,784,036 + LU632_RS14635 0.60 +3.0 2,784,036 + LU632_RS14635 0.60 -0.9 2,784,037 - LU632_RS14635 0.60 +0.1 2,784,041 - LU632_RS14635 0.60 -0.9 2,784,117 + LU632_RS14635 0.69 +0.1 2,784,117 + LU632_RS14635 0.69 -0.0 2,784,117 + LU632_RS14635 0.69 -0.2 2,784,117 + LU632_RS14635 0.69 +0.0 2,784,283 - LU632_RS14635 0.88 +0.0 2,784,346 + +0.2 2,784,347 - -2.0 2,784,416 + +1.0 2,784,416 + +0.2 2,784,416 + -0.6 2,784,416 + +1.1 2,784,434 + +1.3 2,784,435 - +0.2 2,784,435 - -0.7 2,784,558 - cobB LU632_RS14640 0.15 +0.0 2,784,825 + cobB LU632_RS14640 0.47 -0.8 2,784,825 + cobB LU632_RS14640 0.47 +0.0 2,784,825 + cobB LU632_RS14640 0.47 +0.3 2,784,825 + cobB LU632_RS14640 0.47 -0.3 2,784,826 - cobB LU632_RS14640 0.47 -0.8 2,784,826 - cobB LU632_RS14640 0.47 +0.1 2,784,965 - cobB LU632_RS14640 0.64 +0.7 2,785,269 + -1.0 2,785,269 + -0.8 2,785,278 + +0.1
Or see this region's nucleotide sequence