Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14395 and LU632_RS14400 are separated by 11 nucleotides LU632_RS14400 and LU632_RS14405 overlap by 4 nucleotides LU632_RS14405 and LU632_RS14410 overlap by 8 nucleotides
LU632_RS14395: LU632_RS14395 - hypothetical protein, at 2,745,471 to 2,746,109
_RS14395
LU632_RS14400: LU632_RS14400 - class I SAM-dependent methyltransferase, at 2,746,121 to 2,746,735
_RS14400
LU632_RS14405: LU632_RS14405 - glycosyltransferase family 9 protein, at 2,746,732 to 2,747,637
_RS14405
LU632_RS14410: LU632_RS14410 - hypothetical protein, at 2,747,630 to 2,748,172
_RS14410
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2745.791 kb on - strand, within LU632_RS14395 at 2746.140 kb on + strand at 2746.252 kb on + strand, within LU632_RS14400 at 2746.253 kb on - strand, within LU632_RS14400 at 2746.253 kb on - strand, within LU632_RS14400 at 2746.369 kb on + strand, within LU632_RS14400 at 2746.390 kb on + strand, within LU632_RS14400 at 2746.416 kb on + strand, within LU632_RS14400 at 2746.416 kb on + strand, within LU632_RS14400 at 2746.469 kb on - strand, within LU632_RS14400 at 2746.697 kb on + strand at 2746.697 kb on + strand at 2746.698 kb on - strand at 2746.698 kb on - strand at 2746.698 kb on - strand at 2746.760 kb on + strand at 2746.760 kb on + strand at 2746.760 kb on + strand at 2746.859 kb on + strand, within LU632_RS14405 at 2746.859 kb on + strand, within LU632_RS14405 at 2746.863 kb on + strand, within LU632_RS14405 at 2746.864 kb on - strand, within LU632_RS14405 at 2746.984 kb on - strand, within LU632_RS14405 at 2747.183 kb on + strand, within LU632_RS14405 at 2747.183 kb on + strand, within LU632_RS14405 at 2747.184 kb on - strand, within LU632_RS14405 at 2747.184 kb on - strand, within LU632_RS14405 at 2747.191 kb on + strand, within LU632_RS14405 at 2747.292 kb on - strand, within LU632_RS14405 at 2747.300 kb on - strand, within LU632_RS14405 at 2747.415 kb on + strand, within LU632_RS14405 at 2747.416 kb on - strand, within LU632_RS14405 at 2747.510 kb on + strand, within LU632_RS14405 at 2747.588 kb on - strand at 2747.639 kb on + strand at 2747.640 kb on - strand at 2747.640 kb on - strand at 2747.702 kb on + strand, within LU632_RS14410 at 2747.771 kb on - strand, within LU632_RS14410 at 2747.839 kb on - strand, within LU632_RS14410 at 2747.947 kb on - strand, within LU632_RS14410 at 2747.957 kb on - strand, within LU632_RS14410 at 2748.009 kb on + strand, within LU632_RS14410 at 2748.009 kb on + strand, within LU632_RS14410 at 2748.009 kb on + strand, within LU632_RS14410 at 2748.009 kb on + strand, within LU632_RS14410 at 2748.010 kb on - strand, within LU632_RS14410 at 2748.039 kb on + strand, within LU632_RS14410 at 2748.040 kb on - strand, within LU632_RS14410 at 2748.040 kb on - strand, within LU632_RS14410 at 2748.043 kb on + strand, within LU632_RS14410 at 2748.043 kb on + strand, within LU632_RS14410 at 2748.043 kb on + strand, within LU632_RS14410 at 2748.116 kb on + strand, within LU632_RS14410 at 2748.160 kb on + strand at 2748.160 kb on + strand at 2748.161 kb on - strand at 2748.163 kb on + strand at 2748.163 kb on + strand at 2748.163 kb on + strand at 2748.163 kb on + strand at 2748.163 kb on + strand at 2748.164 kb on - strand at 2748.164 kb on - strand at 2748.164 kb on - strand at 2748.167 kb on - strand at 2748.183 kb on - strand at 2748.200 kb on - strand at 2748.201 kb on - strand at 2748.201 kb on - strand at 2748.204 kb on + strand at 2748.205 kb on - strand at 2748.264 kb on - strand at 2748.276 kb on + strand at 2748.294 kb on - strand at 2748.313 kb on + strand at 2748.326 kb on - strand at 2748.352 kb on - strand at 2748.477 kb on + strand at 2748.533 kb on - strand at 2748.542 kb on - strand at 2748.582 kb on + strand at 2748.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,745,791 - LU632_RS14395 0.50 -0.2 2,746,140 + -0.6 2,746,252 + LU632_RS14400 0.21 +0.3 2,746,253 - LU632_RS14400 0.21 -0.1 2,746,253 - LU632_RS14400 0.21 -0.5 2,746,369 + LU632_RS14400 0.40 -0.6 2,746,390 + LU632_RS14400 0.44 -1.3 2,746,416 + LU632_RS14400 0.48 -0.1 2,746,416 + LU632_RS14400 0.48 -0.0 2,746,469 - LU632_RS14400 0.57 -0.5 2,746,697 + -2.1 2,746,697 + -0.2 2,746,698 - +0.4 2,746,698 - -2.1 2,746,698 - +0.5 2,746,760 + -0.3 2,746,760 + +0.3 2,746,760 + +0.0 2,746,859 + LU632_RS14405 0.14 +0.3 2,746,859 + LU632_RS14405 0.14 -0.6 2,746,863 + LU632_RS14405 0.14 -1.0 2,746,864 - LU632_RS14405 0.15 -0.0 2,746,984 - LU632_RS14405 0.28 -0.3 2,747,183 + LU632_RS14405 0.50 +0.0 2,747,183 + LU632_RS14405 0.50 -0.5 2,747,184 - LU632_RS14405 0.50 +0.7 2,747,184 - LU632_RS14405 0.50 -0.3 2,747,191 + LU632_RS14405 0.51 -2.1 2,747,292 - LU632_RS14405 0.62 +0.1 2,747,300 - LU632_RS14405 0.63 +0.5 2,747,415 + LU632_RS14405 0.75 -0.4 2,747,416 - LU632_RS14405 0.75 -0.3 2,747,510 + LU632_RS14405 0.86 -0.2 2,747,588 - -0.3 2,747,639 + +0.2 2,747,640 - -0.3 2,747,640 - +0.1 2,747,702 + LU632_RS14410 0.13 -0.3 2,747,771 - LU632_RS14410 0.26 -0.2 2,747,839 - LU632_RS14410 0.38 +0.3 2,747,947 - LU632_RS14410 0.58 -0.3 2,747,957 - LU632_RS14410 0.60 -0.7 2,748,009 + LU632_RS14410 0.70 -0.0 2,748,009 + LU632_RS14410 0.70 +0.2 2,748,009 + LU632_RS14410 0.70 +0.1 2,748,009 + LU632_RS14410 0.70 -0.2 2,748,010 - LU632_RS14410 0.70 -0.2 2,748,039 + LU632_RS14410 0.75 -1.7 2,748,040 - LU632_RS14410 0.76 +0.2 2,748,040 - LU632_RS14410 0.76 -0.3 2,748,043 + LU632_RS14410 0.76 -0.4 2,748,043 + LU632_RS14410 0.76 -1.0 2,748,043 + LU632_RS14410 0.76 -0.3 2,748,116 + LU632_RS14410 0.90 +0.1 2,748,160 + +0.7 2,748,160 + -0.2 2,748,161 - -0.3 2,748,163 + +0.4 2,748,163 + -0.2 2,748,163 + -0.1 2,748,163 + +1.4 2,748,163 + +2.1 2,748,164 - -1.1 2,748,164 - -0.3 2,748,164 - -0.5 2,748,167 - +0.9 2,748,183 - +0.6 2,748,200 - +0.1 2,748,201 - +0.5 2,748,201 - +0.5 2,748,204 + +3.5 2,748,205 - +0.9 2,748,264 - -1.2 2,748,276 + +1.7 2,748,294 - +0.1 2,748,313 + +0.1 2,748,326 - -0.3 2,748,352 - +0.8 2,748,477 + -0.2 2,748,533 - +0.1 2,748,542 - +0.2 2,748,582 + +0.1 2,748,588 + +0.3
Or see this region's nucleotide sequence