Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpCF are separated by 2 nucleotides trpCF and trpB are separated by 50 nucleotides
LU632_RS12760: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 2,427,929 to 2,429,521
trpD
LU632_RS12765: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 2,429,524 to 2,430,885
trpCF
LU632_RS12770: trpB - tryptophan synthase subunit beta, at 2,430,936 to 2,432,126
trpB
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-2
-1
0
1 at 2428.529 kb on - strand, within trpD at 2428.564 kb on - strand, within trpD at 2428.599 kb on + strand, within trpD at 2428.600 kb on - strand, within trpD at 2428.783 kb on + strand, within trpD at 2428.783 kb on + strand, within trpD at 2428.784 kb on - strand, within trpD at 2428.784 kb on - strand, within trpD at 2428.786 kb on + strand, within trpD at 2428.786 kb on + strand, within trpD at 2428.786 kb on + strand, within trpD at 2428.786 kb on + strand, within trpD at 2428.789 kb on + strand, within trpD at 2428.954 kb on + strand, within trpD at 2428.955 kb on - strand, within trpD at 2428.955 kb on - strand, within trpD at 2429.036 kb on - strand, within trpD at 2429.102 kb on + strand, within trpD at 2429.349 kb on + strand, within trpD at 2429.349 kb on + strand, within trpD at 2429.349 kb on + strand, within trpD at 2429.349 kb on + strand, within trpD at 2429.350 kb on - strand, within trpD at 2429.537 kb on + strand at 2429.537 kb on + strand at 2429.560 kb on - strand at 2429.619 kb on + strand at 2429.620 kb on - strand at 2429.620 kb on - strand at 2429.644 kb on + strand at 2429.673 kb on - strand, within trpCF at 2429.676 kb on + strand, within trpCF at 2429.677 kb on - strand, within trpCF at 2429.800 kb on + strand, within trpCF at 2429.801 kb on - strand, within trpCF at 2429.911 kb on + strand, within trpCF at 2429.912 kb on - strand, within trpCF at 2429.933 kb on + strand, within trpCF at 2429.962 kb on + strand, within trpCF at 2429.988 kb on + strand, within trpCF at 2429.989 kb on - strand, within trpCF at 2429.989 kb on - strand, within trpCF at 2430.030 kb on - strand, within trpCF at 2430.150 kb on + strand, within trpCF at 2430.151 kb on - strand, within trpCF at 2430.178 kb on - strand, within trpCF at 2430.184 kb on + strand, within trpCF at 2430.184 kb on + strand, within trpCF at 2430.298 kb on - strand, within trpCF at 2430.358 kb on + strand, within trpCF at 2430.359 kb on - strand, within trpCF at 2430.441 kb on + strand, within trpCF at 2430.441 kb on + strand, within trpCF at 2430.442 kb on - strand, within trpCF at 2430.442 kb on - strand, within trpCF at 2430.445 kb on + strand, within trpCF at 2430.445 kb on + strand, within trpCF at 2430.446 kb on - strand, within trpCF at 2430.584 kb on + strand, within trpCF at 2430.590 kb on + strand, within trpCF at 2430.609 kb on + strand, within trpCF at 2430.610 kb on - strand, within trpCF at 2430.843 kb on + strand at 2430.844 kb on - strand at 2430.946 kb on + strand at 2430.978 kb on + strand at 2430.978 kb on + strand at 2430.978 kb on + strand at 2430.979 kb on - strand at 2430.979 kb on - strand at 2431.087 kb on - strand, within trpB at 2431.158 kb on - strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.369 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.490 kb on + strand, within trpB at 2431.491 kb on - strand, within trpB at 2431.491 kb on - strand, within trpB at 2431.716 kb on - strand, within trpB at 2431.763 kb on + strand, within trpB at 2431.767 kb on + strand, within trpB at 2431.767 kb on + strand, within trpB at 2431.768 kb on - strand, within trpB at 2431.768 kb on - strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.881 kb on - strand, within trpB
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,428,529 - trpD LU632_RS12760 0.38 +0.3 2,428,564 - trpD LU632_RS12760 0.40 -0.8 2,428,599 + trpD LU632_RS12760 0.42 -0.2 2,428,600 - trpD LU632_RS12760 0.42 +0.2 2,428,783 + trpD LU632_RS12760 0.54 +0.4 2,428,783 + trpD LU632_RS12760 0.54 +0.7 2,428,784 - trpD LU632_RS12760 0.54 -0.6 2,428,784 - trpD LU632_RS12760 0.54 -0.4 2,428,786 + trpD LU632_RS12760 0.54 +0.5 2,428,786 + trpD LU632_RS12760 0.54 +0.7 2,428,786 + trpD LU632_RS12760 0.54 -0.7 2,428,786 + trpD LU632_RS12760 0.54 -0.1 2,428,789 + trpD LU632_RS12760 0.54 +0.8 2,428,954 + trpD LU632_RS12760 0.64 +0.2 2,428,955 - trpD LU632_RS12760 0.64 +0.3 2,428,955 - trpD LU632_RS12760 0.64 -0.1 2,429,036 - trpD LU632_RS12760 0.69 +0.2 2,429,102 + trpD LU632_RS12760 0.74 +0.4 2,429,349 + trpD LU632_RS12760 0.89 +0.2 2,429,349 + trpD LU632_RS12760 0.89 -0.9 2,429,349 + trpD LU632_RS12760 0.89 +0.7 2,429,349 + trpD LU632_RS12760 0.89 -0.7 2,429,350 - trpD LU632_RS12760 0.89 -0.3 2,429,537 + -0.5 2,429,537 + +0.9 2,429,560 - -0.0 2,429,619 + -0.1 2,429,620 - -0.2 2,429,620 - -0.3 2,429,644 + -0.2 2,429,673 - trpCF LU632_RS12765 0.11 -2.1 2,429,676 + trpCF LU632_RS12765 0.11 -0.0 2,429,677 - trpCF LU632_RS12765 0.11 +0.9 2,429,800 + trpCF LU632_RS12765 0.20 -0.7 2,429,801 - trpCF LU632_RS12765 0.20 -0.1 2,429,911 + trpCF LU632_RS12765 0.28 +0.1 2,429,912 - trpCF LU632_RS12765 0.28 -0.6 2,429,933 + trpCF LU632_RS12765 0.30 +0.1 2,429,962 + trpCF LU632_RS12765 0.32 +0.3 2,429,988 + trpCF LU632_RS12765 0.34 -0.3 2,429,989 - trpCF LU632_RS12765 0.34 -1.3 2,429,989 - trpCF LU632_RS12765 0.34 +0.0 2,430,030 - trpCF LU632_RS12765 0.37 -0.3 2,430,150 + trpCF LU632_RS12765 0.46 -0.2 2,430,151 - trpCF LU632_RS12765 0.46 -0.3 2,430,178 - trpCF LU632_RS12765 0.48 +1.2 2,430,184 + trpCF LU632_RS12765 0.48 -1.5 2,430,184 + trpCF LU632_RS12765 0.48 -0.8 2,430,298 - trpCF LU632_RS12765 0.57 -0.3 2,430,358 + trpCF LU632_RS12765 0.61 +0.7 2,430,359 - trpCF LU632_RS12765 0.61 -0.7 2,430,441 + trpCF LU632_RS12765 0.67 -2.3 2,430,441 + trpCF LU632_RS12765 0.67 -1.3 2,430,442 - trpCF LU632_RS12765 0.67 -0.6 2,430,442 - trpCF LU632_RS12765 0.67 +0.9 2,430,445 + trpCF LU632_RS12765 0.68 -0.2 2,430,445 + trpCF LU632_RS12765 0.68 -0.7 2,430,446 - trpCF LU632_RS12765 0.68 -0.7 2,430,584 + trpCF LU632_RS12765 0.78 -1.2 2,430,590 + trpCF LU632_RS12765 0.78 -0.6 2,430,609 + trpCF LU632_RS12765 0.80 -1.9 2,430,610 - trpCF LU632_RS12765 0.80 +0.1 2,430,843 + -0.5 2,430,844 - +0.3 2,430,946 + -1.0 2,430,978 + -1.0 2,430,978 + +0.4 2,430,978 + +0.7 2,430,979 - -0.1 2,430,979 - +0.9 2,431,087 - trpB LU632_RS12770 0.13 +0.1 2,431,158 - trpB LU632_RS12770 0.19 -1.5 2,431,329 + trpB LU632_RS12770 0.33 +0.9 2,431,329 + trpB LU632_RS12770 0.33 -1.5 2,431,329 + trpB LU632_RS12770 0.33 -0.5 2,431,369 + trpB LU632_RS12770 0.36 +0.2 2,431,488 + trpB LU632_RS12770 0.46 -0.9 2,431,488 + trpB LU632_RS12770 0.46 -0.1 2,431,488 + trpB LU632_RS12770 0.46 -0.5 2,431,488 + trpB LU632_RS12770 0.46 -0.6 2,431,488 + trpB LU632_RS12770 0.46 -0.5 2,431,489 - trpB LU632_RS12770 0.46 -1.3 2,431,489 - trpB LU632_RS12770 0.46 -0.1 2,431,489 - trpB LU632_RS12770 0.46 -0.9 2,431,489 - trpB LU632_RS12770 0.46 -1.0 2,431,489 - trpB LU632_RS12770 0.46 -0.9 2,431,490 + trpB LU632_RS12770 0.47 -0.6 2,431,491 - trpB LU632_RS12770 0.47 +0.4 2,431,491 - trpB LU632_RS12770 0.47 -0.6 2,431,716 - trpB LU632_RS12770 0.65 +0.7 2,431,763 + trpB LU632_RS12770 0.69 -0.1 2,431,767 + trpB LU632_RS12770 0.70 -0.1 2,431,767 + trpB LU632_RS12770 0.70 -0.3 2,431,768 - trpB LU632_RS12770 0.70 -2.3 2,431,768 - trpB LU632_RS12770 0.70 -0.6 2,431,824 + trpB LU632_RS12770 0.75 -0.8 2,431,824 + trpB LU632_RS12770 0.75 -1.0 2,431,824 + trpB LU632_RS12770 0.75 -0.1 2,431,881 - trpB LU632_RS12770 0.79 -1.2
Or see this region's nucleotide sequence