Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS12615 and LU632_RS12620 overlap by 12 nucleotides LU632_RS12620 and LU632_RS12625 are separated by 72 nucleotides LU632_RS12625 and hrpA are separated by 314 nucleotides
LU632_RS12615: LU632_RS12615 - oxidoreductase, at 2,399,206 to 2,400,369
_RS12615
LU632_RS12620: LU632_RS12620 - hypothetical protein, at 2,400,358 to 2,400,663
_RS12620
LU632_RS12625: LU632_RS12625 - methyl-accepting chemotaxis protein, at 2,400,736 to 2,402,292
_RS12625
LU632_RS12630: hrpA - ATP-dependent RNA helicase HrpA, at 2,402,607 to 2,406,503
hrpA
Position (kb)
2400
2401
2402
2403 Strain fitness (log2 ratio)
-1
0
1 at 2399.769 kb on - strand, within LU632_RS12615 at 2399.936 kb on + strand, within LU632_RS12615 at 2399.936 kb on + strand, within LU632_RS12615 at 2399.997 kb on - strand, within LU632_RS12615 at 2400.072 kb on + strand, within LU632_RS12615 at 2400.086 kb on + strand, within LU632_RS12615 at 2400.103 kb on - strand, within LU632_RS12615 at 2400.233 kb on + strand, within LU632_RS12615 at 2400.242 kb on + strand, within LU632_RS12615 at 2400.344 kb on + strand at 2400.345 kb on - strand at 2400.379 kb on + strand at 2400.379 kb on + strand at 2400.380 kb on - strand at 2400.380 kb on - strand at 2400.582 kb on + strand, within LU632_RS12620 at 2400.583 kb on - strand, within LU632_RS12620 at 2400.598 kb on - strand, within LU632_RS12620 at 2400.737 kb on + strand at 2400.738 kb on - strand at 2400.802 kb on - strand at 2400.876 kb on + strand at 2400.877 kb on - strand at 2400.942 kb on + strand, within LU632_RS12625 at 2400.943 kb on - strand, within LU632_RS12625 at 2400.943 kb on - strand, within LU632_RS12625 at 2400.943 kb on - strand, within LU632_RS12625 at 2400.970 kb on - strand, within LU632_RS12625 at 2401.006 kb on + strand, within LU632_RS12625 at 2401.174 kb on - strand, within LU632_RS12625 at 2401.175 kb on - strand, within LU632_RS12625 at 2401.177 kb on - strand, within LU632_RS12625 at 2401.179 kb on + strand, within LU632_RS12625 at 2401.206 kb on + strand, within LU632_RS12625 at 2401.212 kb on + strand, within LU632_RS12625 at 2401.245 kb on + strand, within LU632_RS12625 at 2401.350 kb on + strand, within LU632_RS12625 at 2401.351 kb on - strand, within LU632_RS12625 at 2401.400 kb on - strand, within LU632_RS12625 at 2401.530 kb on + strand, within LU632_RS12625 at 2401.838 kb on + strand, within LU632_RS12625 at 2401.857 kb on + strand, within LU632_RS12625 at 2401.947 kb on + strand, within LU632_RS12625 at 2401.947 kb on + strand, within LU632_RS12625 at 2401.948 kb on - strand, within LU632_RS12625 at 2402.028 kb on + strand, within LU632_RS12625 at 2402.059 kb on - strand, within LU632_RS12625 at 2402.322 kb on + strand at 2402.324 kb on + strand at 2402.329 kb on - strand at 2402.370 kb on - strand at 2402.374 kb on - strand at 2402.399 kb on + strand at 2402.541 kb on - strand at 2402.683 kb on + strand at 2402.715 kb on + strand at 2402.716 kb on - strand at 2402.759 kb on + strand at 2402.771 kb on + strand at 2402.797 kb on - strand at 2402.882 kb on + strand at 2402.912 kb on + strand at 2402.982 kb on - strand at 2403.008 kb on + strand, within hrpA at 2403.009 kb on - strand, within hrpA at 2403.009 kb on - strand, within hrpA at 2403.018 kb on + strand, within hrpA at 2403.030 kb on + strand, within hrpA at 2403.051 kb on + strand, within hrpA at 2403.052 kb on - strand, within hrpA at 2403.052 kb on - strand, within hrpA at 2403.052 kb on - strand, within hrpA
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,399,769 - LU632_RS12615 0.48 -0.3 2,399,936 + LU632_RS12615 0.63 +0.1 2,399,936 + LU632_RS12615 0.63 +0.1 2,399,997 - LU632_RS12615 0.68 -0.2 2,400,072 + LU632_RS12615 0.74 +1.7 2,400,086 + LU632_RS12615 0.76 +0.7 2,400,103 - LU632_RS12615 0.77 +0.8 2,400,233 + LU632_RS12615 0.88 -0.3 2,400,242 + LU632_RS12615 0.89 -0.1 2,400,344 + -0.3 2,400,345 - +0.2 2,400,379 + +0.1 2,400,379 + -0.3 2,400,380 - +0.7 2,400,380 - -1.4 2,400,582 + LU632_RS12620 0.73 -0.9 2,400,583 - LU632_RS12620 0.74 -0.4 2,400,598 - LU632_RS12620 0.78 +0.1 2,400,737 + +0.4 2,400,738 - +0.7 2,400,802 - -0.2 2,400,876 + +0.3 2,400,877 - -1.2 2,400,942 + LU632_RS12625 0.13 +0.1 2,400,943 - LU632_RS12625 0.13 +0.0 2,400,943 - LU632_RS12625 0.13 +0.3 2,400,943 - LU632_RS12625 0.13 -0.3 2,400,970 - LU632_RS12625 0.15 +0.3 2,401,006 + LU632_RS12625 0.17 +0.5 2,401,174 - LU632_RS12625 0.28 +0.7 2,401,175 - LU632_RS12625 0.28 -0.5 2,401,177 - LU632_RS12625 0.28 -0.3 2,401,179 + LU632_RS12625 0.28 +0.6 2,401,206 + LU632_RS12625 0.30 -0.3 2,401,212 + LU632_RS12625 0.31 -0.1 2,401,245 + LU632_RS12625 0.33 -1.1 2,401,350 + LU632_RS12625 0.39 -0.2 2,401,351 - LU632_RS12625 0.39 +0.7 2,401,400 - LU632_RS12625 0.43 +0.5 2,401,530 + LU632_RS12625 0.51 +0.7 2,401,838 + LU632_RS12625 0.71 +0.3 2,401,857 + LU632_RS12625 0.72 +0.8 2,401,947 + LU632_RS12625 0.78 -0.8 2,401,947 + LU632_RS12625 0.78 +1.4 2,401,948 - LU632_RS12625 0.78 -0.2 2,402,028 + LU632_RS12625 0.83 +0.2 2,402,059 - LU632_RS12625 0.85 +0.2 2,402,322 + +0.1 2,402,324 + +0.2 2,402,329 - -0.7 2,402,370 - -0.1 2,402,374 - +0.4 2,402,399 + -0.7 2,402,541 - -0.2 2,402,683 + +0.7 2,402,715 + +0.4 2,402,716 - -0.7 2,402,759 + -0.2 2,402,771 + +0.2 2,402,797 - +0.4 2,402,882 + -0.9 2,402,912 + -0.8 2,402,982 - +0.4 2,403,008 + hrpA LU632_RS12630 0.10 -0.9 2,403,009 - hrpA LU632_RS12630 0.10 -0.4 2,403,009 - hrpA LU632_RS12630 0.10 -0.3 2,403,018 + hrpA LU632_RS12630 0.11 +0.1 2,403,030 + hrpA LU632_RS12630 0.11 -0.2 2,403,051 + hrpA LU632_RS12630 0.11 -0.9 2,403,052 - hrpA LU632_RS12630 0.11 +0.5 2,403,052 - hrpA LU632_RS12630 0.11 +0.2 2,403,052 - hrpA LU632_RS12630 0.11 +0.7
Or see this region's nucleotide sequence