Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zntB and LU632_RS12215 are separated by 328 nucleotides LU632_RS12215 and LU632_RS12220 are separated by 78 nucleotides LU632_RS12220 and LU632_RS12225 are separated by 104 nucleotides
LU632_RS12210: zntB - zinc transporter ZntB, at 2,317,534 to 2,318,517
zntB
LU632_RS12215: LU632_RS12215 - PTS transporter subunit EIIB, at 2,318,846 to 2,319,178
_RS12215
LU632_RS12220: LU632_RS12220 - IS481 family transposase, at 2,319,257 to 2,320,297
_RS12220
LU632_RS12225: LU632_RS12225 - glucose PTS transporter subunit IIA, at 2,320,402 to 2,321,949
_RS12225
Position (kb)
2319
2320
2321 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2318.259 kb on + strand, within zntB at 2318.260 kb on - strand, within zntB at 2318.260 kb on - strand, within zntB at 2318.260 kb on - strand, within zntB at 2318.347 kb on + strand, within zntB at 2318.376 kb on + strand, within zntB at 2318.389 kb on + strand, within zntB at 2318.389 kb on + strand, within zntB at 2318.416 kb on - strand, within zntB at 2318.451 kb on + strand at 2318.473 kb on - strand at 2318.529 kb on - strand at 2318.556 kb on + strand at 2318.581 kb on - strand at 2318.581 kb on - strand at 2318.596 kb on - strand at 2318.672 kb on - strand at 2318.683 kb on + strand at 2318.697 kb on - strand at 2318.701 kb on + strand at 2318.824 kb on - strand at 2318.853 kb on - strand at 2318.853 kb on - strand at 2318.874 kb on + strand at 2318.875 kb on - strand at 2318.995 kb on + strand, within LU632_RS12215 at 2319.033 kb on + strand, within LU632_RS12215 at 2319.040 kb on - strand, within LU632_RS12215 at 2319.040 kb on - strand, within LU632_RS12215 at 2319.044 kb on - strand, within LU632_RS12215 at 2319.047 kb on + strand, within LU632_RS12215 at 2319.047 kb on + strand, within LU632_RS12215 at 2319.047 kb on + strand, within LU632_RS12215 at 2319.047 kb on + strand, within LU632_RS12215 at 2319.047 kb on + strand, within LU632_RS12215 at 2320.392 kb on + strand at 2320.393 kb on - strand at 2320.488 kb on - strand at 2320.511 kb on + strand at 2320.530 kb on - strand at 2320.541 kb on - strand at 2320.602 kb on - strand, within LU632_RS12225 at 2320.693 kb on + strand, within LU632_RS12225 at 2320.693 kb on + strand, within LU632_RS12225 at 2320.693 kb on + strand, within LU632_RS12225 at 2320.693 kb on + strand, within LU632_RS12225 at 2320.694 kb on - strand, within LU632_RS12225 at 2320.694 kb on - strand, within LU632_RS12225 at 2320.786 kb on - strand, within LU632_RS12225 at 2320.802 kb on + strand, within LU632_RS12225 at 2320.802 kb on + strand, within LU632_RS12225 at 2320.803 kb on - strand, within LU632_RS12225 at 2320.803 kb on - strand, within LU632_RS12225 at 2320.900 kb on + strand, within LU632_RS12225 at 2320.928 kb on - strand, within LU632_RS12225 at 2321.015 kb on - strand, within LU632_RS12225 at 2321.106 kb on + strand, within LU632_RS12225 at 2321.171 kb on + strand, within LU632_RS12225 at 2321.174 kb on - strand, within LU632_RS12225 at 2321.174 kb on - strand, within LU632_RS12225 at 2321.182 kb on - strand, within LU632_RS12225 at 2321.187 kb on - strand, within LU632_RS12225 at 2321.187 kb on - strand, within LU632_RS12225 at 2321.187 kb on - strand, within LU632_RS12225 at 2321.190 kb on + strand, within LU632_RS12225 at 2321.269 kb on + strand, within LU632_RS12225 at 2321.269 kb on + strand, within LU632_RS12225 at 2321.270 kb on - strand, within LU632_RS12225 at 2321.270 kb on - strand, within LU632_RS12225 at 2321.287 kb on - strand, within LU632_RS12225 at 2321.290 kb on - strand, within LU632_RS12225 at 2321.290 kb on - strand, within LU632_RS12225 at 2321.291 kb on + strand, within LU632_RS12225 at 2321.292 kb on - strand, within LU632_RS12225 at 2321.292 kb on - strand, within LU632_RS12225 at 2321.295 kb on + strand, within LU632_RS12225
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,318,259 + zntB LU632_RS12210 0.74 -0.4 2,318,260 - zntB LU632_RS12210 0.74 -0.5 2,318,260 - zntB LU632_RS12210 0.74 -1.3 2,318,260 - zntB LU632_RS12210 0.74 +0.1 2,318,347 + zntB LU632_RS12210 0.83 -1.1 2,318,376 + zntB LU632_RS12210 0.86 -5.9 2,318,389 + zntB LU632_RS12210 0.87 -0.1 2,318,389 + zntB LU632_RS12210 0.87 -0.3 2,318,416 - zntB LU632_RS12210 0.90 +0.2 2,318,451 + +0.0 2,318,473 - +0.2 2,318,529 - -1.3 2,318,556 + -0.7 2,318,581 - -0.3 2,318,581 - +0.3 2,318,596 - -0.3 2,318,672 - -1.3 2,318,683 + +1.2 2,318,697 - -0.8 2,318,701 + +0.9 2,318,824 - -0.0 2,318,853 - +0.8 2,318,853 - -0.1 2,318,874 + -2.1 2,318,875 - +0.1 2,318,995 + LU632_RS12215 0.45 -1.0 2,319,033 + LU632_RS12215 0.56 +0.8 2,319,040 - LU632_RS12215 0.58 -1.5 2,319,040 - LU632_RS12215 0.58 -0.1 2,319,044 - LU632_RS12215 0.59 -0.1 2,319,047 + LU632_RS12215 0.60 +0.2 2,319,047 + LU632_RS12215 0.60 +0.2 2,319,047 + LU632_RS12215 0.60 -1.1 2,319,047 + LU632_RS12215 0.60 -0.2 2,319,047 + LU632_RS12215 0.60 +0.6 2,320,392 + -0.3 2,320,393 - -1.2 2,320,488 - +0.3 2,320,511 + -0.4 2,320,530 - +0.4 2,320,541 - +0.4 2,320,602 - LU632_RS12225 0.13 -0.6 2,320,693 + LU632_RS12225 0.19 -0.1 2,320,693 + LU632_RS12225 0.19 +0.3 2,320,693 + LU632_RS12225 0.19 +0.3 2,320,693 + LU632_RS12225 0.19 -0.1 2,320,694 - LU632_RS12225 0.19 +0.2 2,320,694 - LU632_RS12225 0.19 -0.3 2,320,786 - LU632_RS12225 0.25 +1.7 2,320,802 + LU632_RS12225 0.26 -0.9 2,320,802 + LU632_RS12225 0.26 +0.0 2,320,803 - LU632_RS12225 0.26 -0.7 2,320,803 - LU632_RS12225 0.26 +0.4 2,320,900 + LU632_RS12225 0.32 +1.2 2,320,928 - LU632_RS12225 0.34 -0.9 2,321,015 - LU632_RS12225 0.40 -0.1 2,321,106 + LU632_RS12225 0.45 -0.5 2,321,171 + LU632_RS12225 0.50 -0.3 2,321,174 - LU632_RS12225 0.50 +0.3 2,321,174 - LU632_RS12225 0.50 +1.5 2,321,182 - LU632_RS12225 0.50 -0.2 2,321,187 - LU632_RS12225 0.51 -1.2 2,321,187 - LU632_RS12225 0.51 -0.3 2,321,187 - LU632_RS12225 0.51 +2.0 2,321,190 + LU632_RS12225 0.51 -0.3 2,321,269 + LU632_RS12225 0.56 +0.7 2,321,269 + LU632_RS12225 0.56 -0.1 2,321,270 - LU632_RS12225 0.56 -1.0 2,321,270 - LU632_RS12225 0.56 -1.2 2,321,287 - LU632_RS12225 0.57 -0.2 2,321,290 - LU632_RS12225 0.57 -0.2 2,321,290 - LU632_RS12225 0.57 +0.4 2,321,291 + LU632_RS12225 0.57 -0.2 2,321,292 - LU632_RS12225 0.57 -0.4 2,321,292 - LU632_RS12225 0.57 +0.2 2,321,295 + LU632_RS12225 0.58 +1.9
Or see this region's nucleotide sequence