Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsA and fliR are separated by 645 nucleotides fliR and fliQ overlap by 1 nucleotides fliQ and fliP are separated by 141 nucleotides
LU632_RS10510: rcsA - transcriptional regulator RcsA, at 2,009,851 to 2,010,486
rcsA
LU632_RS10515: fliR - flagellar type III secretion system protein FliR, at 2,011,132 to 2,011,917
fliR
LU632_RS10520: fliQ - flagellar biosynthesis protein FliQ, at 2,011,917 to 2,012,186
fliQ
LU632_RS10525: fliP - flagellar type III secretion system pore protein FliP, at 2,012,328 to 2,013,062
fliP
Position (kb)
2011
2012 Strain fitness (log2 ratio)
-1
0
1
2 at 2010.200 kb on + strand, within rcsA at 2010.544 kb on - strand at 2010.906 kb on + strand at 2010.913 kb on + strand at 2010.994 kb on - strand at 2011.129 kb on + strand at 2011.177 kb on + strand at 2011.234 kb on - strand, within fliR at 2011.237 kb on + strand, within fliR at 2011.412 kb on + strand, within fliR at 2011.453 kb on - strand, within fliR at 2011.479 kb on + strand, within fliR at 2011.480 kb on - strand, within fliR at 2011.684 kb on + strand, within fliR at 2011.684 kb on + strand, within fliR at 2011.685 kb on - strand, within fliR at 2011.756 kb on + strand, within fliR at 2011.768 kb on - strand, within fliR at 2011.768 kb on - strand, within fliR at 2011.768 kb on - strand, within fliR at 2011.768 kb on - strand, within fliR at 2011.801 kb on - strand, within fliR at 2011.803 kb on + strand, within fliR at 2011.807 kb on - strand, within fliR at 2011.837 kb on + strand, within fliR at 2011.837 kb on + strand, within fliR at 2011.837 kb on + strand, within fliR at 2011.870 kb on + strand at 2011.912 kb on + strand at 2011.943 kb on + strand at 2011.944 kb on - strand, within fliQ at 2011.956 kb on + strand, within fliQ at 2011.957 kb on - strand, within fliQ at 2011.957 kb on - strand, within fliQ at 2011.958 kb on + strand, within fliQ at 2011.958 kb on + strand, within fliQ at 2011.959 kb on - strand, within fliQ at 2011.960 kb on + strand, within fliQ at 2011.961 kb on - strand, within fliQ at 2011.961 kb on - strand, within fliQ at 2011.977 kb on + strand, within fliQ at 2012.034 kb on - strand, within fliQ at 2012.160 kb on + strand at 2012.161 kb on - strand at 2012.214 kb on + strand at 2012.251 kb on + strand at 2012.251 kb on + strand at 2012.329 kb on + strand at 2012.360 kb on + strand at 2012.364 kb on + strand at 2012.365 kb on - strand at 2012.365 kb on - strand at 2012.403 kb on - strand, within fliP at 2012.416 kb on - strand, within fliP at 2012.416 kb on - strand, within fliP at 2012.416 kb on - strand, within fliP at 2012.417 kb on + strand, within fliP at 2012.546 kb on - strand, within fliP at 2012.546 kb on - strand, within fliP at 2012.561 kb on + strand, within fliP at 2012.561 kb on + strand, within fliP at 2012.562 kb on - strand, within fliP at 2012.654 kb on + strand, within fliP at 2012.655 kb on - strand, within fliP at 2012.765 kb on + strand, within fliP at 2012.768 kb on + strand, within fliP at 2012.769 kb on - strand, within fliP at 2012.802 kb on + strand, within fliP at 2012.802 kb on + strand, within fliP at 2012.861 kb on + strand, within fliP
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,010,200 + rcsA LU632_RS10510 0.55 +0.3 2,010,544 - -0.1 2,010,906 + +0.4 2,010,913 + +0.9 2,010,994 - +0.3 2,011,129 + +0.0 2,011,177 + -0.7 2,011,234 - fliR LU632_RS10515 0.13 +0.1 2,011,237 + fliR LU632_RS10515 0.13 -0.2 2,011,412 + fliR LU632_RS10515 0.36 -0.3 2,011,453 - fliR LU632_RS10515 0.41 +2.0 2,011,479 + fliR LU632_RS10515 0.44 +0.4 2,011,480 - fliR LU632_RS10515 0.44 -0.5 2,011,684 + fliR LU632_RS10515 0.70 -0.2 2,011,684 + fliR LU632_RS10515 0.70 -0.2 2,011,685 - fliR LU632_RS10515 0.70 +0.4 2,011,756 + fliR LU632_RS10515 0.79 +0.0 2,011,768 - fliR LU632_RS10515 0.81 +0.8 2,011,768 - fliR LU632_RS10515 0.81 -0.5 2,011,768 - fliR LU632_RS10515 0.81 -0.1 2,011,768 - fliR LU632_RS10515 0.81 +0.9 2,011,801 - fliR LU632_RS10515 0.85 -0.4 2,011,803 + fliR LU632_RS10515 0.85 +0.2 2,011,807 - fliR LU632_RS10515 0.86 +0.1 2,011,837 + fliR LU632_RS10515 0.90 +0.1 2,011,837 + fliR LU632_RS10515 0.90 -0.1 2,011,837 + fliR LU632_RS10515 0.90 +0.1 2,011,870 + +0.1 2,011,912 + +0.1 2,011,943 + +0.1 2,011,944 - fliQ LU632_RS10520 0.10 -0.0 2,011,956 + fliQ LU632_RS10520 0.14 -0.4 2,011,957 - fliQ LU632_RS10520 0.15 +0.3 2,011,957 - fliQ LU632_RS10520 0.15 -0.6 2,011,958 + fliQ LU632_RS10520 0.15 +0.0 2,011,958 + fliQ LU632_RS10520 0.15 -0.7 2,011,959 - fliQ LU632_RS10520 0.16 +0.1 2,011,960 + fliQ LU632_RS10520 0.16 +0.4 2,011,961 - fliQ LU632_RS10520 0.16 +0.8 2,011,961 - fliQ LU632_RS10520 0.16 -0.5 2,011,977 + fliQ LU632_RS10520 0.22 -0.1 2,012,034 - fliQ LU632_RS10520 0.43 +0.8 2,012,160 + -0.5 2,012,161 - -0.5 2,012,214 + -0.1 2,012,251 + -0.6 2,012,251 + +0.2 2,012,329 + -0.5 2,012,360 + +0.1 2,012,364 + +0.2 2,012,365 - +0.3 2,012,365 - +0.3 2,012,403 - fliP LU632_RS10525 0.10 -1.0 2,012,416 - fliP LU632_RS10525 0.12 +0.6 2,012,416 - fliP LU632_RS10525 0.12 +0.1 2,012,416 - fliP LU632_RS10525 0.12 -0.0 2,012,417 + fliP LU632_RS10525 0.12 +0.2 2,012,546 - fliP LU632_RS10525 0.30 +0.5 2,012,546 - fliP LU632_RS10525 0.30 -0.1 2,012,561 + fliP LU632_RS10525 0.32 +0.4 2,012,561 + fliP LU632_RS10525 0.32 -0.1 2,012,562 - fliP LU632_RS10525 0.32 -0.6 2,012,654 + fliP LU632_RS10525 0.44 -0.5 2,012,655 - fliP LU632_RS10525 0.44 +0.2 2,012,765 + fliP LU632_RS10525 0.59 -0.3 2,012,768 + fliP LU632_RS10525 0.60 -0.3 2,012,769 - fliP LU632_RS10525 0.60 +2.2 2,012,802 + fliP LU632_RS10525 0.64 +0.7 2,012,802 + fliP LU632_RS10525 0.64 -0.1 2,012,861 + fliP LU632_RS10525 0.73 +0.3
Or see this region's nucleotide sequence