Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10030 and LU632_RS10035 are separated by 48 nucleotides LU632_RS10035 and LU632_RS10040 are separated by 39 nucleotides LU632_RS10040 and LU632_RS10045 are separated by 147 nucleotides LU632_RS10045 and helD are separated by 131 nucleotides
LU632_RS10030: LU632_RS10030 - IS1 family transposase, at 1,919,218 to 1,919,915
_RS10030
LU632_RS10035: LU632_RS10035 - YccS/YhfK family membrane protein, at 1,919,964 to 1,920,691
_RS10035
LU632_RS10040: LU632_RS10040 - IS3 family transposase, at 1,920,731 to 1,921,932
_RS10040
LU632_RS10045: LU632_RS10045 - YccF domain-containing protein, at 1,922,080 to 1,922,526
_RS10045
LU632_RS10050: helD - DNA helicase IV, at 1,922,658 to 1,924,712
helD
Position (kb)
1920
1921
1922 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1919.940 kb on + strand at 1919.979 kb on + strand at 1920.000 kb on + strand at 1920.001 kb on - strand at 1920.003 kb on - strand at 1920.020 kb on + strand at 1920.093 kb on + strand, within LU632_RS10035 at 1920.097 kb on - strand, within LU632_RS10035 at 1920.097 kb on - strand, within LU632_RS10035 at 1920.097 kb on - strand, within LU632_RS10035 at 1920.102 kb on - strand, within LU632_RS10035 at 1920.153 kb on - strand, within LU632_RS10035 at 1920.153 kb on - strand, within LU632_RS10035 at 1920.216 kb on - strand, within LU632_RS10035 at 1920.216 kb on - strand, within LU632_RS10035 at 1920.317 kb on + strand, within LU632_RS10035 at 1920.318 kb on - strand, within LU632_RS10035 at 1920.318 kb on - strand, within LU632_RS10035 at 1920.350 kb on - strand, within LU632_RS10035 at 1920.367 kb on - strand, within LU632_RS10035 at 1920.367 kb on - strand, within LU632_RS10035 at 1920.406 kb on - strand, within LU632_RS10035 at 1920.439 kb on + strand, within LU632_RS10035 at 1920.441 kb on + strand, within LU632_RS10035 at 1920.441 kb on + strand, within LU632_RS10035 at 1920.441 kb on + strand, within LU632_RS10035 at 1920.442 kb on - strand, within LU632_RS10035 at 1920.442 kb on - strand, within LU632_RS10035 at 1920.541 kb on + strand, within LU632_RS10035 at 1920.542 kb on - strand, within LU632_RS10035 at 1920.584 kb on + strand, within LU632_RS10035 at 1920.619 kb on + strand at 1920.623 kb on + strand at 1920.624 kb on - strand at 1920.671 kb on + strand at 1920.671 kb on + strand at 1920.672 kb on - strand at 1920.691 kb on + strand at 1920.691 kb on + strand at 1920.692 kb on - strand at 1921.934 kb on + strand at 1921.937 kb on + strand at 1922.038 kb on + strand at 1922.039 kb on - strand at 1922.047 kb on - strand at 1922.061 kb on + strand at 1922.105 kb on - strand at 1922.126 kb on - strand, within LU632_RS10045 at 1922.136 kb on + strand, within LU632_RS10045 at 1922.137 kb on - strand, within LU632_RS10045 at 1922.141 kb on - strand, within LU632_RS10045 at 1922.242 kb on + strand, within LU632_RS10045 at 1922.243 kb on - strand, within LU632_RS10045 at 1922.243 kb on - strand, within LU632_RS10045 at 1922.243 kb on - strand, within LU632_RS10045 at 1922.243 kb on - strand, within LU632_RS10045 at 1922.285 kb on - strand, within LU632_RS10045 at 1922.289 kb on + strand, within LU632_RS10045 at 1922.290 kb on - strand, within LU632_RS10045 at 1922.367 kb on + strand, within LU632_RS10045 at 1922.526 kb on + strand at 1922.553 kb on + strand at 1922.554 kb on - strand at 1922.639 kb on + strand at 1922.639 kb on + strand at 1922.640 kb on - strand at 1922.685 kb on - strand at 1922.707 kb on - strand at 1922.763 kb on + strand at 1922.763 kb on + strand at 1922.763 kb on + strand at 1922.764 kb on - strand at 1922.764 kb on - strand at 1922.765 kb on + strand at 1922.765 kb on + strand at 1922.765 kb on + strand at 1922.765 kb on + strand at 1922.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,919,940 + -0.2 1,919,979 + -0.1 1,920,000 + -1.3 1,920,001 - +0.1 1,920,003 - -0.3 1,920,020 + -0.0 1,920,093 + LU632_RS10035 0.18 +0.7 1,920,097 - LU632_RS10035 0.18 +0.4 1,920,097 - LU632_RS10035 0.18 +0.7 1,920,097 - LU632_RS10035 0.18 +0.2 1,920,102 - LU632_RS10035 0.19 +2.3 1,920,153 - LU632_RS10035 0.26 -0.2 1,920,153 - LU632_RS10035 0.26 +0.4 1,920,216 - LU632_RS10035 0.35 +0.2 1,920,216 - LU632_RS10035 0.35 +0.5 1,920,317 + LU632_RS10035 0.48 -0.1 1,920,318 - LU632_RS10035 0.49 +0.1 1,920,318 - LU632_RS10035 0.49 -0.5 1,920,350 - LU632_RS10035 0.53 -0.5 1,920,367 - LU632_RS10035 0.55 +0.3 1,920,367 - LU632_RS10035 0.55 +0.0 1,920,406 - LU632_RS10035 0.61 +0.4 1,920,439 + LU632_RS10035 0.65 +0.4 1,920,441 + LU632_RS10035 0.66 -0.6 1,920,441 + LU632_RS10035 0.66 -0.4 1,920,441 + LU632_RS10035 0.66 +0.0 1,920,442 - LU632_RS10035 0.66 -0.8 1,920,442 - LU632_RS10035 0.66 +0.2 1,920,541 + LU632_RS10035 0.79 +0.2 1,920,542 - LU632_RS10035 0.79 -0.3 1,920,584 + LU632_RS10035 0.85 +0.8 1,920,619 + -2.1 1,920,623 + -0.5 1,920,624 - +0.9 1,920,671 + -0.6 1,920,671 + +0.4 1,920,672 - +0.4 1,920,691 + -0.0 1,920,691 + +0.2 1,920,692 - -0.9 1,921,934 + +0.2 1,921,937 + -0.5 1,922,038 + +0.4 1,922,039 - +0.0 1,922,047 - -0.5 1,922,061 + +0.8 1,922,105 - +0.3 1,922,126 - LU632_RS10045 0.10 -0.5 1,922,136 + LU632_RS10045 0.13 -1.4 1,922,137 - LU632_RS10045 0.13 -0.3 1,922,141 - LU632_RS10045 0.14 +0.6 1,922,242 + LU632_RS10045 0.36 -0.1 1,922,243 - LU632_RS10045 0.36 -2.3 1,922,243 - LU632_RS10045 0.36 +0.9 1,922,243 - LU632_RS10045 0.36 -0.2 1,922,243 - LU632_RS10045 0.36 -0.1 1,922,285 - LU632_RS10045 0.46 -0.5 1,922,289 + LU632_RS10045 0.47 -0.1 1,922,290 - LU632_RS10045 0.47 +0.1 1,922,367 + LU632_RS10045 0.64 -1.2 1,922,526 + -1.4 1,922,553 + -0.3 1,922,554 - +0.9 1,922,639 + +0.5 1,922,639 + +0.3 1,922,640 - -0.3 1,922,685 - -0.3 1,922,707 - -1.5 1,922,763 + -0.6 1,922,763 + -0.6 1,922,763 + -0.3 1,922,764 - -0.7 1,922,764 - -1.1 1,922,765 + -0.3 1,922,765 + -1.0 1,922,765 + +0.5 1,922,765 + -1.3 1,922,766 - -1.3
Or see this region's nucleotide sequence