Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and LU632_RS09935 are separated by 259 nucleotides LU632_RS09935 and LU632_RS09940 are separated by 15 nucleotides LU632_RS09940 and LU632_RS09945 are separated by 52 nucleotides LU632_RS09945 and ssuE are separated by 10 nucleotides
LU632_RS09930: pepN - aminopeptidase N, at 1,896,432 to 1,899,047
pepN
LU632_RS09935: LU632_RS09935 - aliphatic sulfonate ABC transporter substrate-binding protein, at 1,899,307 to 1,899,450
_RS09935
LU632_RS09940: LU632_RS09940 - IS1 family transposase, at 1,899,466 to 1,900,163
_RS09940
LU632_RS09945: LU632_RS09945 - aliphatic sulfonate ABC transporter substrate-binding protein, at 1,900,216 to 1,900,416
_RS09945
LU632_RS09950: ssuE - NADPH-dependent FMN reductase, at 1,900,427 to 1,900,968
ssuE
Position (kb)
1899
1900
1901 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1898.624 kb on - strand, within pepN at 1898.687 kb on - strand, within pepN at 1898.922 kb on - strand at 1898.942 kb on + strand at 1898.956 kb on + strand at 1899.167 kb on + strand at 1899.259 kb on + strand at 1899.259 kb on + strand at 1899.259 kb on + strand at 1899.260 kb on - strand at 1899.263 kb on - strand at 1899.263 kb on - strand at 1899.306 kb on - strand at 1899.336 kb on - strand, within LU632_RS09935 at 1899.370 kb on - strand, within LU632_RS09935 at 1899.378 kb on + strand, within LU632_RS09935 at 1900.546 kb on + strand, within ssuE at 1900.569 kb on - strand, within ssuE at 1900.640 kb on + strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.641 kb on - strand, within ssuE at 1900.681 kb on + strand, within ssuE at 1900.726 kb on - strand, within ssuE at 1900.740 kb on + strand, within ssuE at 1900.849 kb on + strand, within ssuE at 1900.852 kb on - strand, within ssuE at 1900.853 kb on + strand, within ssuE at 1900.854 kb on - strand, within ssuE at 1900.957 kb on - strand at 1900.960 kb on - strand at 1900.973 kb on - strand at 1901.023 kb on + strand at 1901.050 kb on + strand at 1901.050 kb on + strand at 1901.088 kb on + strand at 1901.088 kb on + strand at 1901.092 kb on + strand at 1901.092 kb on + strand at 1901.100 kb on + strand at 1901.131 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,898,624 - pepN LU632_RS09930 0.84 +1.7 1,898,687 - pepN LU632_RS09930 0.86 -0.2 1,898,922 - -0.7 1,898,942 + +0.5 1,898,956 + -0.0 1,899,167 + +0.2 1,899,259 + -0.3 1,899,259 + -0.1 1,899,259 + -1.2 1,899,260 - -0.6 1,899,263 - +0.1 1,899,263 - -0.8 1,899,306 - -0.1 1,899,336 - LU632_RS09935 0.20 +0.9 1,899,370 - LU632_RS09935 0.44 -0.2 1,899,378 + LU632_RS09935 0.49 -0.3 1,900,546 + ssuE LU632_RS09950 0.22 +0.9 1,900,569 - ssuE LU632_RS09950 0.26 +0.1 1,900,640 + ssuE LU632_RS09950 0.39 +0.3 1,900,641 - ssuE LU632_RS09950 0.39 +1.3 1,900,641 - ssuE LU632_RS09950 0.39 -0.9 1,900,641 - ssuE LU632_RS09950 0.39 -1.4 1,900,641 - ssuE LU632_RS09950 0.39 -0.2 1,900,641 - ssuE LU632_RS09950 0.39 -0.3 1,900,641 - ssuE LU632_RS09950 0.39 +0.7 1,900,681 + ssuE LU632_RS09950 0.47 +0.2 1,900,726 - ssuE LU632_RS09950 0.55 -0.0 1,900,740 + ssuE LU632_RS09950 0.58 -0.6 1,900,849 + ssuE LU632_RS09950 0.78 -0.0 1,900,852 - ssuE LU632_RS09950 0.78 +2.2 1,900,853 + ssuE LU632_RS09950 0.79 +0.0 1,900,854 - ssuE LU632_RS09950 0.79 -2.3 1,900,957 - -0.5 1,900,960 - -0.3 1,900,973 - -0.4 1,901,023 + -0.3 1,901,050 + +0.4 1,901,050 + +0.7 1,901,088 + +0.3 1,901,088 + +0.6 1,901,092 + -0.6 1,901,092 + +0.5 1,901,100 + +0.1 1,901,131 + -0.2
Or see this region's nucleotide sequence