Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmC and LU632_RS09665 are separated by 64 nucleotides LU632_RS09665 and artM are separated by 256 nucleotides artM and artQ overlap by 7 nucleotides artQ and artJ are separated by 7 nucleotides
LU632_RS09660: rlmC - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 1,830,974 to 1,832,101
rlmC
LU632_RS09665: LU632_RS09665 - transporter substrate-binding domain-containing protein, at 1,832,166 to 1,832,683
_RS09665
LU632_RS09670: artM - arginine ABC transporter permease ArtM, at 1,832,940 to 1,833,608
artM
LU632_RS09675: artQ - arginine ABC transporter permease ArtQ, at 1,833,602 to 1,834,324
artQ
LU632_RS09680: artJ - arginine ABC transporter substrate-binding protein, at 1,834,332 to 1,835,063
artJ
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1831.962 kb on + strand, within rlmC at 1831.962 kb on + strand, within rlmC at 1831.962 kb on + strand, within rlmC at 1831.962 kb on + strand, within rlmC at 1831.963 kb on - strand, within rlmC at 1831.963 kb on - strand, within rlmC at 1832.020 kb on + strand at 1832.020 kb on + strand at 1832.020 kb on + strand at 1832.020 kb on + strand at 1832.021 kb on - strand at 1832.021 kb on - strand at 1832.048 kb on - strand at 1832.050 kb on - strand at 1832.189 kb on + strand at 1832.190 kb on - strand at 1832.358 kb on - strand, within LU632_RS09665 at 1832.400 kb on + strand, within LU632_RS09665 at 1832.400 kb on + strand, within LU632_RS09665 at 1832.401 kb on - strand, within LU632_RS09665 at 1832.403 kb on - strand, within LU632_RS09665 at 1832.403 kb on - strand, within LU632_RS09665 at 1832.460 kb on + strand, within LU632_RS09665 at 1832.527 kb on + strand, within LU632_RS09665 at 1832.528 kb on - strand, within LU632_RS09665 at 1832.535 kb on - strand, within LU632_RS09665 at 1832.571 kb on + strand, within LU632_RS09665 at 1832.571 kb on + strand, within LU632_RS09665 at 1832.572 kb on - strand, within LU632_RS09665 at 1832.572 kb on - strand, within LU632_RS09665 at 1832.595 kb on - strand, within LU632_RS09665 at 1832.675 kb on + strand at 1832.675 kb on + strand at 1832.676 kb on - strand at 1832.745 kb on + strand at 1832.746 kb on - strand at 1832.810 kb on - strand at 1832.813 kb on + strand at 1832.844 kb on + strand at 1832.845 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.910 kb on - strand at 1832.988 kb on + strand at 1833.020 kb on - strand, within artM at 1833.055 kb on + strand, within artM at 1833.056 kb on - strand, within artM at 1833.125 kb on + strand, within artM at 1833.125 kb on + strand, within artM at 1833.126 kb on - strand, within artM at 1833.142 kb on - strand, within artM at 1833.211 kb on - strand, within artM at 1833.216 kb on - strand, within artM at 1833.404 kb on - strand, within artM at 1833.404 kb on - strand, within artM at 1833.406 kb on + strand, within artM at 1833.406 kb on + strand, within artM at 1833.407 kb on - strand, within artM at 1833.595 kb on - strand at 1833.599 kb on - strand at 1833.599 kb on - strand at 1833.655 kb on + strand at 1833.656 kb on - strand at 1833.656 kb on - strand at 1833.690 kb on + strand, within artQ at 1833.695 kb on - strand, within artQ at 1833.709 kb on + strand, within artQ at 1833.710 kb on - strand, within artQ at 1833.711 kb on + strand, within artQ at 1833.746 kb on - strand, within artQ at 1833.746 kb on - strand, within artQ at 1833.763 kb on + strand, within artQ at 1833.982 kb on - strand, within artQ at 1834.312 kb on - strand at 1834.354 kb on + strand at 1834.354 kb on + strand at 1834.354 kb on + strand at 1834.355 kb on - strand at 1834.355 kb on - strand at 1834.417 kb on - strand, within artJ at 1834.598 kb on - strand, within artJ at 1834.607 kb on - strand, within artJ
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,831,962 + rlmC LU632_RS09660 0.88 +0.5 1,831,962 + rlmC LU632_RS09660 0.88 +0.1 1,831,962 + rlmC LU632_RS09660 0.88 +0.5 1,831,962 + rlmC LU632_RS09660 0.88 +0.2 1,831,963 - rlmC LU632_RS09660 0.88 -0.2 1,831,963 - rlmC LU632_RS09660 0.88 +1.5 1,832,020 + +1.2 1,832,020 + +1.2 1,832,020 + -0.1 1,832,020 + -0.9 1,832,021 - +0.7 1,832,021 - +0.6 1,832,048 - -0.2 1,832,050 - -0.2 1,832,189 + +0.6 1,832,190 - -0.6 1,832,358 - LU632_RS09665 0.37 +0.7 1,832,400 + LU632_RS09665 0.45 +0.2 1,832,400 + LU632_RS09665 0.45 -1.1 1,832,401 - LU632_RS09665 0.45 +0.4 1,832,403 - LU632_RS09665 0.46 +1.2 1,832,403 - LU632_RS09665 0.46 -0.0 1,832,460 + LU632_RS09665 0.57 +0.6 1,832,527 + LU632_RS09665 0.70 +0.3 1,832,528 - LU632_RS09665 0.70 -0.0 1,832,535 - LU632_RS09665 0.71 -0.1 1,832,571 + LU632_RS09665 0.78 -0.3 1,832,571 + LU632_RS09665 0.78 -0.3 1,832,572 - LU632_RS09665 0.78 +0.5 1,832,572 - LU632_RS09665 0.78 +0.1 1,832,595 - LU632_RS09665 0.83 -1.6 1,832,675 + +3.3 1,832,675 + +0.3 1,832,676 - -0.9 1,832,745 + -1.4 1,832,746 - -0.5 1,832,810 - -0.9 1,832,813 + +1.5 1,832,844 + +0.2 1,832,845 - +0.4 1,832,910 - +0.2 1,832,910 - -0.3 1,832,910 - +0.3 1,832,910 - -0.2 1,832,910 - -2.5 1,832,910 - +0.6 1,832,910 - +0.3 1,832,988 + -0.9 1,833,020 - artM LU632_RS09670 0.12 -0.3 1,833,055 + artM LU632_RS09670 0.17 -1.1 1,833,056 - artM LU632_RS09670 0.17 -0.0 1,833,125 + artM LU632_RS09670 0.28 +0.5 1,833,125 + artM LU632_RS09670 0.28 -0.7 1,833,126 - artM LU632_RS09670 0.28 -1.3 1,833,142 - artM LU632_RS09670 0.30 -0.7 1,833,211 - artM LU632_RS09670 0.41 +0.3 1,833,216 - artM LU632_RS09670 0.41 -1.1 1,833,404 - artM LU632_RS09670 0.69 +0.0 1,833,404 - artM LU632_RS09670 0.69 +0.4 1,833,406 + artM LU632_RS09670 0.70 -0.7 1,833,406 + artM LU632_RS09670 0.70 +0.1 1,833,407 - artM LU632_RS09670 0.70 +0.3 1,833,595 - -0.7 1,833,599 - +0.3 1,833,599 - +1.4 1,833,655 + +0.4 1,833,656 - -0.3 1,833,656 - -0.1 1,833,690 + artQ LU632_RS09675 0.12 -1.5 1,833,695 - artQ LU632_RS09675 0.13 +0.1 1,833,709 + artQ LU632_RS09675 0.15 +0.2 1,833,710 - artQ LU632_RS09675 0.15 +1.7 1,833,711 + artQ LU632_RS09675 0.15 +0.7 1,833,746 - artQ LU632_RS09675 0.20 -0.9 1,833,746 - artQ LU632_RS09675 0.20 +0.5 1,833,763 + artQ LU632_RS09675 0.22 -0.9 1,833,982 - artQ LU632_RS09675 0.53 +0.7 1,834,312 - -0.5 1,834,354 + +0.3 1,834,354 + -0.0 1,834,354 + +0.7 1,834,355 - -0.2 1,834,355 - -0.1 1,834,417 - artJ LU632_RS09680 0.12 +0.1 1,834,598 - artJ LU632_RS09680 0.36 -0.5 1,834,607 - artJ LU632_RS09680 0.38 -0.4
Or see this region's nucleotide sequence