Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS08910 and hisIE are separated by 322 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH are separated by 5 nucleotides
LU632_RS08910: LU632_RS08910 - hypothetical protein, at 1,682,848 to 1,683,159
_RS08910
LU632_RS08915: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,683,482 to 1,684,093
hisIE
LU632_RS08920: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,684,087 to 1,684,863
hisF
LU632_RS08925: hisA - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,684,845 to 1,685,582
hisA
LU632_RS08930: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,685,588 to 1,686,178
hisH
Position (kb)
1684
1685 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1683.106 kb on - strand, within LU632_RS08910 at 1683.191 kb on - strand at 1683.211 kb on + strand at 1683.212 kb on - strand at 1683.261 kb on + strand at 1683.269 kb on + strand at 1683.313 kb on - strand at 1683.318 kb on - strand at 1683.355 kb on + strand at 1683.385 kb on - strand at 1683.441 kb on + strand at 1683.442 kb on - strand at 1683.448 kb on - strand at 1683.473 kb on + strand at 1683.561 kb on + strand, within hisIE at 1683.562 kb on - strand, within hisIE at 1683.562 kb on - strand, within hisIE at 1683.985 kb on + strand, within hisIE at 1683.985 kb on + strand, within hisIE at 1683.986 kb on - strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.988 kb on - strand, within hisIE at 1684.042 kb on + strand at 1684.043 kb on - strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.149 kb on + strand at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.264 kb on - strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.336 kb on - strand, within hisF at 1684.431 kb on - strand, within hisF at 1684.445 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.467 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.801 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1685.234 kb on + strand, within hisA at 1685.234 kb on + strand, within hisA at 1685.471 kb on - strand, within hisA at 1685.478 kb on + strand, within hisA at 1685.503 kb on + strand, within hisA at 1685.576 kb on + strand at 1685.589 kb on + strand at 1685.590 kb on - strand at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.707 kb on + strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.760 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,683,106 - LU632_RS08910 0.83 +1.8 1,683,191 - -0.5 1,683,211 + -0.2 1,683,212 - +1.0 1,683,261 + -0.9 1,683,269 + -0.3 1,683,313 - -0.9 1,683,318 - +0.0 1,683,355 + -1.0 1,683,385 - +0.2 1,683,441 + -0.6 1,683,442 - +0.2 1,683,448 - -1.0 1,683,473 + +0.5 1,683,561 + hisIE LU632_RS08915 0.13 -1.8 1,683,562 - hisIE LU632_RS08915 0.13 -2.6 1,683,562 - hisIE LU632_RS08915 0.13 +0.3 1,683,985 + hisIE LU632_RS08915 0.82 -2.7 1,683,985 + hisIE LU632_RS08915 0.82 -2.4 1,683,986 - hisIE LU632_RS08915 0.82 -0.2 1,683,987 + hisIE LU632_RS08915 0.83 -2.2 1,683,987 + hisIE LU632_RS08915 0.83 -4.1 1,683,988 - hisIE LU632_RS08915 0.83 -2.0 1,684,042 + -2.9 1,684,043 - -3.2 1,684,146 + -1.8 1,684,146 + -2.4 1,684,146 + -2.7 1,684,149 + -1.5 1,684,263 + hisF LU632_RS08920 0.23 -2.3 1,684,263 + hisF LU632_RS08920 0.23 -1.2 1,684,263 + hisF LU632_RS08920 0.23 -1.2 1,684,264 - hisF LU632_RS08920 0.23 -2.8 1,684,332 + hisF LU632_RS08920 0.32 -3.6 1,684,332 + hisF LU632_RS08920 0.32 -4.7 1,684,336 - hisF LU632_RS08920 0.32 -5.3 1,684,431 - hisF LU632_RS08920 0.44 -2.9 1,684,445 + hisF LU632_RS08920 0.46 -2.0 1,684,466 + hisF LU632_RS08920 0.49 -4.0 1,684,466 + hisF LU632_RS08920 0.49 -0.8 1,684,467 - hisF LU632_RS08920 0.49 -1.5 1,684,589 - hisF LU632_RS08920 0.65 -2.0 1,684,589 - hisF LU632_RS08920 0.65 -1.5 1,684,589 - hisF LU632_RS08920 0.65 -0.8 1,684,801 + -2.4 1,684,866 + -1.8 1,684,866 + -1.8 1,684,866 + -0.8 1,685,234 + hisA LU632_RS08925 0.53 -1.5 1,685,234 + hisA LU632_RS08925 0.53 -1.8 1,685,471 - hisA LU632_RS08925 0.85 -3.3 1,685,478 + hisA LU632_RS08925 0.86 -1.5 1,685,503 + hisA LU632_RS08925 0.89 -2.5 1,685,576 + -3.4 1,685,589 + -2.4 1,685,590 - -0.8 1,685,703 + hisH LU632_RS08930 0.19 -1.8 1,685,703 + hisH LU632_RS08930 0.19 -2.9 1,685,703 + hisH LU632_RS08930 0.19 -1.2 1,685,704 - hisH LU632_RS08930 0.20 -4.5 1,685,704 - hisH LU632_RS08930 0.20 -4.8 1,685,707 + hisH LU632_RS08930 0.20 -1.5 1,685,708 - hisH LU632_RS08930 0.20 -3.9 1,685,708 - hisH LU632_RS08930 0.20 -1.7 1,685,708 - hisH LU632_RS08930 0.20 -2.7 1,685,708 - hisH LU632_RS08930 0.20 -2.5 1,685,708 - hisH LU632_RS08930 0.20 -5.1 1,685,745 + hisH LU632_RS08930 0.27 -4.2 1,685,745 + hisH LU632_RS08930 0.27 -2.5 1,685,746 - hisH LU632_RS08930 0.27 -3.4 1,685,746 - hisH LU632_RS08930 0.27 -3.9 1,685,746 - hisH LU632_RS08930 0.27 -1.8 1,685,760 + hisH LU632_RS08930 0.29 -2.4 1,685,766 + hisH LU632_RS08930 0.30 +0.8 1,685,766 + hisH LU632_RS08930 0.30 -2.5 1,685,767 - hisH LU632_RS08930 0.30 -2.4 1,685,767 - hisH LU632_RS08930 0.30 -5.4 1,685,767 - hisH LU632_RS08930 0.30 -3.8
Or see this region's nucleotide sequence