Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS08095 and elaB are separated by 190 nucleotides elaB and menD are separated by 332 nucleotides menD and menH overlap by 4 nucleotides menH and menB are separated by 9 nucleotides
LU632_RS08095: LU632_RS08095 - glyoxalase, at 1,515,460 to 1,515,696
_RS08095
LU632_RS08100: elaB - stress response protein ElaB, at 1,515,887 to 1,516,198
elaB
LU632_RS08105: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 1,516,531 to 1,518,192
menD
LU632_RS08110: menH - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 1,518,189 to 1,518,953
menH
LU632_RS08115: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 1,518,963 to 1,519,820
menB
Position (kb) 1516
1517
1518
1519 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1515.550 kb on + strand, within LU632_RS08095 at 1515.581 kb on - strand, within LU632_RS08095 at 1515.581 kb on - strand, within LU632_RS08095 at 1515.581 kb on - strand, within LU632_RS08095 at 1515.581 kb on - strand, within LU632_RS08095 at 1515.581 kb on - strand, within LU632_RS08095 at 1515.727 kb on - strand at 1515.728 kb on + strand at 1515.780 kb on + strand at 1515.803 kb on + strand at 1516.073 kb on + strand, within elaB at 1516.074 kb on - strand, within elaB at 1516.074 kb on - strand, within elaB at 1516.077 kb on - strand, within elaB at 1516.077 kb on - strand, within elaB at 1516.080 kb on - strand, within elaB at 1516.099 kb on + strand, within elaB at 1516.100 kb on - strand, within elaB at 1516.233 kb on + strand at 1516.243 kb on + strand at 1516.256 kb on + strand at 1516.257 kb on - strand at 1516.409 kb on - strand at 1516.417 kb on + strand at 1516.488 kb on + strand at 1516.488 kb on + strand at 1516.496 kb on + strand at 1516.496 kb on + strand at 1516.496 kb on + strand at 1516.498 kb on + strand at 1516.531 kb on - strand at 1516.531 kb on - strand at 1516.599 kb on - strand at 1516.599 kb on - strand at 1516.665 kb on + strand at 1516.666 kb on - strand at 1516.812 kb on + strand, within menD at 1516.887 kb on + strand, within menD at 1516.887 kb on + strand, within menD at 1516.932 kb on + strand, within menD at 1516.933 kb on - strand, within menD at 1517.153 kb on + strand, within menD at 1517.412 kb on + strand, within menD at 1517.412 kb on + strand, within menD at 1517.413 kb on - strand, within menD at 1517.489 kb on - strand, within menD at 1517.517 kb on + strand, within menD at 1517.758 kb on + strand, within menD at 1517.759 kb on - strand, within menD at 1517.759 kb on - strand, within menD at 1517.959 kb on - strand, within menD at 1518.052 kb on + strand at 1518.053 kb on - strand at 1518.053 kb on - strand at 1518.053 kb on - strand at 1518.055 kb on + strand at 1518.140 kb on + strand at 1518.395 kb on - strand, within menH at 1518.395 kb on - strand, within menH at 1518.395 kb on - strand, within menH at 1518.471 kb on + strand, within menH at 1518.471 kb on + strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.475 kb on - strand, within menH at 1518.532 kb on - strand, within menH at 1518.548 kb on + strand, within menH at 1518.637 kb on - strand, within menH at 1518.662 kb on + strand, within menH at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.951 kb on + strand at 1519.003 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,515,550 + LU632_RS08095 0.38 +0.0 1,515,581 - LU632_RS08095 0.51 -0.3 1,515,581 - LU632_RS08095 0.51 -0.4 1,515,581 - LU632_RS08095 0.51 -0.3 1,515,581 - LU632_RS08095 0.51 -1.2 1,515,581 - LU632_RS08095 0.51 -0.0 1,515,727 - +0.3 1,515,728 + +0.0 1,515,780 + +0.8 1,515,803 + -0.0 1,516,073 + elaB LU632_RS08100 0.60 -0.1 1,516,074 - elaB LU632_RS08100 0.60 +0.2 1,516,074 - elaB LU632_RS08100 0.60 +0.4 1,516,077 - elaB LU632_RS08100 0.61 +0.1 1,516,077 - elaB LU632_RS08100 0.61 -1.0 1,516,080 - elaB LU632_RS08100 0.62 +0.4 1,516,099 + elaB LU632_RS08100 0.68 -0.0 1,516,100 - elaB LU632_RS08100 0.68 +0.4 1,516,233 + -1.4 1,516,243 + -1.7 1,516,256 + +0.1 1,516,257 - -0.3 1,516,409 - +0.5 1,516,417 + -0.3 1,516,488 + -0.0 1,516,488 + +1.2 1,516,496 + +1.1 1,516,496 + -0.1 1,516,496 + -0.5 1,516,498 + -0.2 1,516,531 - -0.3 1,516,531 - +0.4 1,516,599 - -2.0 1,516,599 - -0.3 1,516,665 + +1.2 1,516,666 - +0.7 1,516,812 + menD LU632_RS08105 0.17 -2.1 1,516,887 + menD LU632_RS08105 0.21 -0.1 1,516,887 + menD LU632_RS08105 0.21 +0.8 1,516,932 + menD LU632_RS08105 0.24 -0.3 1,516,933 - menD LU632_RS08105 0.24 +2.1 1,517,153 + menD LU632_RS08105 0.37 +0.1 1,517,412 + menD LU632_RS08105 0.53 +0.2 1,517,412 + menD LU632_RS08105 0.53 +0.8 1,517,413 - menD LU632_RS08105 0.53 +0.4 1,517,489 - menD LU632_RS08105 0.58 -1.4 1,517,517 + menD LU632_RS08105 0.59 +0.7 1,517,758 + menD LU632_RS08105 0.74 +0.5 1,517,759 - menD LU632_RS08105 0.74 -0.0 1,517,759 - menD LU632_RS08105 0.74 -1.8 1,517,959 - menD LU632_RS08105 0.86 +0.2 1,518,052 + -0.0 1,518,053 - -0.3 1,518,053 - -0.4 1,518,053 - -1.1 1,518,055 + -0.1 1,518,140 + -1.9 1,518,395 - menH LU632_RS08110 0.27 +0.1 1,518,395 - menH LU632_RS08110 0.27 +0.0 1,518,395 - menH LU632_RS08110 0.27 -0.6 1,518,471 + menH LU632_RS08110 0.37 -1.2 1,518,471 + menH LU632_RS08110 0.37 +0.8 1,518,472 - menH LU632_RS08110 0.37 +0.8 1,518,472 - menH LU632_RS08110 0.37 -0.2 1,518,472 - menH LU632_RS08110 0.37 +0.2 1,518,472 - menH LU632_RS08110 0.37 +0.3 1,518,475 - menH LU632_RS08110 0.37 -0.0 1,518,532 - menH LU632_RS08110 0.45 +0.1 1,518,548 + menH LU632_RS08110 0.47 +0.4 1,518,637 - menH LU632_RS08110 0.59 -0.9 1,518,662 + menH LU632_RS08110 0.62 +0.6 1,518,942 - -0.6 1,518,942 - -1.3 1,518,942 - -0.1 1,518,951 + -2.7 1,519,003 + -1.2
Or see this region's nucleotide sequence