Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS06385 and queE are separated by 115 nucleotides queE and queD are separated by 16 nucleotides queD and cysJ are separated by 305 nucleotides
LU632_RS06385: LU632_RS06385 - hypothetical protein, at 1,227,390 to 1,228,067
_RS06385
LU632_RS06390: queE - 7-carboxy-7-deazaguanine synthase QueE, at 1,228,183 to 1,228,854
queE
LU632_RS06395: queD - 6-carboxytetrahydropterin synthase QueD, at 1,228,871 to 1,229,233
queD
LU632_RS06400: cysJ - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 1,229,539 to 1,231,332
cysJ
Position (kb) 1228
1229
1230 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1227.906 kb on + strand, within LU632_RS06385 at 1227.906 kb on + strand, within LU632_RS06385 at 1227.906 kb on + strand, within LU632_RS06385 at 1227.906 kb on + strand, within LU632_RS06385 at 1227.907 kb on - strand, within LU632_RS06385 at 1227.909 kb on - strand, within LU632_RS06385 at 1227.956 kb on - strand, within LU632_RS06385 at 1228.061 kb on + strand at 1228.061 kb on + strand at 1228.061 kb on + strand at 1228.062 kb on - strand at 1228.065 kb on + strand at 1228.065 kb on - strand at 1228.066 kb on - strand at 1228.094 kb on - strand at 1228.146 kb on + strand at 1228.234 kb on + strand at 1228.234 kb on + strand at 1228.235 kb on - strand at 1228.235 kb on - strand at 1228.413 kb on + strand, within queE at 1228.414 kb on - strand, within queE at 1228.414 kb on - strand, within queE at 1228.502 kb on - strand, within queE at 1228.552 kb on - strand, within queE at 1228.552 kb on - strand, within queE at 1228.602 kb on + strand, within queE at 1228.603 kb on - strand, within queE at 1228.603 kb on - strand, within queE at 1228.655 kb on + strand, within queE at 1228.655 kb on + strand, within queE at 1228.656 kb on - strand, within queE at 1228.786 kb on - strand, within queE at 1228.806 kb on + strand at 1228.834 kb on - strand at 1228.837 kb on + strand at 1228.842 kb on + strand at 1228.843 kb on - strand at 1228.845 kb on + strand at 1228.845 kb on + strand at 1229.009 kb on - strand, within queD at 1229.019 kb on + strand, within queD at 1229.019 kb on + strand, within queD at 1229.019 kb on + strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.041 kb on - strand, within queD at 1229.043 kb on - strand, within queD at 1229.131 kb on + strand, within queD at 1229.132 kb on - strand, within queD at 1229.162 kb on - strand, within queD at 1229.162 kb on - strand, within queD at 1229.219 kb on + strand at 1229.396 kb on - strand at 1229.435 kb on + strand at 1229.451 kb on + strand at 1229.465 kb on + strand at 1229.466 kb on - strand at 1229.653 kb on + strand at 1229.653 kb on + strand at 1229.654 kb on - strand at 1229.724 kb on - strand, within cysJ at 1229.784 kb on - strand, within cysJ at 1229.799 kb on + strand, within cysJ at 1229.800 kb on - strand, within cysJ at 1229.829 kb on + strand, within cysJ at 1229.998 kb on + strand, within cysJ
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 1,227,906 + LU632_RS06385 0.76 +0.8 1,227,906 + LU632_RS06385 0.76 -0.7 1,227,906 + LU632_RS06385 0.76 +0.6 1,227,906 + LU632_RS06385 0.76 +0.1 1,227,907 - LU632_RS06385 0.76 +0.1 1,227,909 - LU632_RS06385 0.77 -1.7 1,227,956 - LU632_RS06385 0.83 -0.9 1,228,061 + -0.2 1,228,061 + -0.2 1,228,061 + -0.5 1,228,062 - +1.3 1,228,065 + -0.4 1,228,065 - +0.0 1,228,066 - -0.4 1,228,094 - +0.2 1,228,146 + +0.9 1,228,234 + +1.3 1,228,234 + +0.2 1,228,235 - +0.3 1,228,235 - +0.3 1,228,413 + queE LU632_RS06390 0.34 -0.3 1,228,414 - queE LU632_RS06390 0.34 +0.2 1,228,414 - queE LU632_RS06390 0.34 +0.6 1,228,502 - queE LU632_RS06390 0.47 +0.6 1,228,552 - queE LU632_RS06390 0.55 +1.7 1,228,552 - queE LU632_RS06390 0.55 +2.5 1,228,602 + queE LU632_RS06390 0.62 -0.4 1,228,603 - queE LU632_RS06390 0.62 +0.3 1,228,603 - queE LU632_RS06390 0.62 -0.1 1,228,655 + queE LU632_RS06390 0.70 +0.6 1,228,655 + queE LU632_RS06390 0.70 -0.1 1,228,656 - queE LU632_RS06390 0.70 -0.1 1,228,786 - queE LU632_RS06390 0.90 +0.9 1,228,806 + +0.2 1,228,834 - +0.0 1,228,837 + +1.3 1,228,842 + -0.3 1,228,843 - +0.0 1,228,845 + -0.3 1,228,845 + -0.3 1,229,009 - queD LU632_RS06395 0.38 +1.3 1,229,019 + queD LU632_RS06395 0.41 +0.9 1,229,019 + queD LU632_RS06395 0.41 -0.1 1,229,019 + queD LU632_RS06395 0.41 +1.1 1,229,020 - queD LU632_RS06395 0.41 +0.3 1,229,020 - queD LU632_RS06395 0.41 +0.1 1,229,020 - queD LU632_RS06395 0.41 +0.8 1,229,020 - queD LU632_RS06395 0.41 +0.9 1,229,020 - queD LU632_RS06395 0.41 +0.1 1,229,040 + queD LU632_RS06395 0.47 +0.9 1,229,040 + queD LU632_RS06395 0.47 +0.2 1,229,040 + queD LU632_RS06395 0.47 +0.4 1,229,040 + queD LU632_RS06395 0.47 -0.1 1,229,041 - queD LU632_RS06395 0.47 -0.1 1,229,043 - queD LU632_RS06395 0.47 -0.0 1,229,131 + queD LU632_RS06395 0.72 -0.3 1,229,132 - queD LU632_RS06395 0.72 -0.4 1,229,162 - queD LU632_RS06395 0.80 +0.1 1,229,162 - queD LU632_RS06395 0.80 +0.5 1,229,219 + +0.3 1,229,396 - -0.7 1,229,435 + -1.0 1,229,451 + -0.2 1,229,465 + -1.2 1,229,466 - -3.0 1,229,653 + -3.0 1,229,653 + -3.9 1,229,654 - -3.1 1,229,724 - cysJ LU632_RS06400 0.10 -1.1 1,229,784 - cysJ LU632_RS06400 0.14 -2.4 1,229,799 + cysJ LU632_RS06400 0.14 -2.6 1,229,800 - cysJ LU632_RS06400 0.15 -3.7 1,229,829 + cysJ LU632_RS06400 0.16 -2.9 1,229,998 + cysJ LU632_RS06400 0.26 -0.7
Or see this region's nucleotide sequence