Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04275 and araD overlap by 4 nucleotides araD and LU632_RS04285 are separated by 89 nucleotides LU632_RS04285 and LU632_RS04290 are separated by 582 nucleotides
LU632_RS04275: LU632_RS04275 - L-ribulose-5-phosphate 3-epimerase, at 850,343 to 851,200
_RS04275
LU632_RS04280: araD - L-ribulose-5-phosphate 4-epimerase, at 851,197 to 851,895
araD
LU632_RS04285: LU632_RS04285 - IS5 family transposase, at 851,985 to 852,801
_RS04285
LU632_RS04290: LU632_RS04290 - hypothetical protein, at 853,384 to 854,223
_RS04290
Position (kb) 851
852
853 Strain fitness (log2 ratio) -2
-1
0
1
2 at 851.256 kb on + strand at 851.256 kb on + strand at 851.257 kb on - strand at 851.257 kb on - strand at 851.341 kb on + strand, within araD at 851.364 kb on + strand, within araD at 851.364 kb on + strand, within araD at 851.365 kb on - strand, within araD at 851.365 kb on - strand, within araD at 851.399 kb on + strand, within araD at 851.485 kb on - strand, within araD at 851.485 kb on - strand, within araD at 851.621 kb on - strand, within araD at 851.626 kb on + strand, within araD at 851.627 kb on - strand, within araD at 851.627 kb on - strand, within araD at 851.647 kb on - strand, within araD at 851.648 kb on + strand, within araD at 851.649 kb on - strand, within araD at 851.649 kb on - strand, within araD at 851.670 kb on + strand, within araD at 851.730 kb on + strand, within araD at 851.762 kb on + strand, within araD at 851.763 kb on - strand, within araD at 851.782 kb on + strand, within araD at 851.782 kb on + strand, within araD at 851.782 kb on + strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.855 kb on - strand at 851.882 kb on - strand at 851.896 kb on + strand at 851.899 kb on + strand at 852.832 kb on + strand at 852.832 kb on + strand at 852.832 kb on + strand at 852.832 kb on + strand at 852.832 kb on + strand at 852.832 kb on + strand at 852.834 kb on + strand at 852.961 kb on - strand at 853.049 kb on + strand at 853.165 kb on + strand at 853.165 kb on + strand at 853.165 kb on + strand at 853.169 kb on + strand at 853.169 kb on + strand at 853.170 kb on - strand at 853.170 kb on - strand at 853.743 kb on + strand, within LU632_RS04290 at 853.744 kb on - strand, within LU632_RS04290 at 853.771 kb on + strand, within LU632_RS04290 at 853.772 kb on - strand, within LU632_RS04290
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 851,256 + +0.0 851,256 + +0.3 851,257 - +0.2 851,257 - -0.2 851,341 + araD LU632_RS04280 0.21 -0.9 851,364 + araD LU632_RS04280 0.24 +0.1 851,364 + araD LU632_RS04280 0.24 -1.5 851,365 - araD LU632_RS04280 0.24 -0.2 851,365 - araD LU632_RS04280 0.24 +0.8 851,399 + araD LU632_RS04280 0.29 -0.9 851,485 - araD LU632_RS04280 0.41 -0.5 851,485 - araD LU632_RS04280 0.41 +0.4 851,621 - araD LU632_RS04280 0.61 +1.8 851,626 + araD LU632_RS04280 0.61 -0.2 851,627 - araD LU632_RS04280 0.62 +0.8 851,627 - araD LU632_RS04280 0.62 -0.4 851,647 - araD LU632_RS04280 0.64 +0.6 851,648 + araD LU632_RS04280 0.65 -2.2 851,649 - araD LU632_RS04280 0.65 +0.4 851,649 - araD LU632_RS04280 0.65 +0.6 851,670 + araD LU632_RS04280 0.68 +0.5 851,730 + araD LU632_RS04280 0.76 +0.4 851,762 + araD LU632_RS04280 0.81 -0.3 851,763 - araD LU632_RS04280 0.81 +0.1 851,782 + araD LU632_RS04280 0.84 -0.0 851,782 + araD LU632_RS04280 0.84 +1.3 851,782 + araD LU632_RS04280 0.84 -0.3 851,783 - araD LU632_RS04280 0.84 -0.1 851,783 - araD LU632_RS04280 0.84 -0.6 851,783 - araD LU632_RS04280 0.84 -0.3 851,783 - araD LU632_RS04280 0.84 -0.1 851,783 - araD LU632_RS04280 0.84 -0.4 851,855 - -0.1 851,882 - -0.2 851,896 + +0.4 851,899 + +0.5 852,832 + +0.1 852,832 + -0.6 852,832 + +0.7 852,832 + +0.4 852,832 + +0.2 852,832 + +1.1 852,834 + +0.9 852,961 - +0.2 853,049 + -1.4 853,165 + +0.9 853,165 + -1.2 853,165 + -0.3 853,169 + -0.2 853,169 + -0.1 853,170 - +0.0 853,170 - -0.8 853,743 + LU632_RS04290 0.43 +0.7 853,744 - LU632_RS04290 0.43 +0.2 853,771 + LU632_RS04290 0.46 -1.0 853,772 - LU632_RS04290 0.46 -0.1
Or see this region's nucleotide sequence