Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04130 and LU632_RS04135 are separated by 52 nucleotides LU632_RS04135 and LU632_RS04140 overlap by 7 nucleotides LU632_RS04140 and istA are separated by 231 nucleotides istA and istB are separated by 10 nucleotides
LU632_RS04130: LU632_RS04130 - type IV toxin-antitoxin system YeeU family antitoxin, at 819,873 to 820,232
_RS04130
LU632_RS04135: LU632_RS04135 - toxin, at 820,285 to 820,461
_RS04135
LU632_RS04140: LU632_RS04140 - terminase family protein, at 820,455 to 820,766
_RS04140
LU632_RS04145: istA - IS21 family transposase, at 820,998 to 822,527
istA
LU632_RS04150: istB - IS21-like element helper ATPase IstB, at 822,538 to 823,281
istB
Position (kb) 820
821
822
823 Strain fitness (log2 ratio) -2
-1
0
1 at 820.010 kb on + strand, within LU632_RS04130 at 820.010 kb on + strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.141 kb on - strand, within LU632_RS04130 at 820.191 kb on - strand, within LU632_RS04130 at 820.194 kb on + strand, within LU632_RS04130 at 820.195 kb on - strand, within LU632_RS04130 at 820.253 kb on + strand at 820.254 kb on - strand at 820.292 kb on + strand at 820.292 kb on + strand at 820.354 kb on - strand, within LU632_RS04135 at 820.375 kb on - strand, within LU632_RS04135 at 820.446 kb on + strand at 820.455 kb on - strand at 820.496 kb on + strand, within LU632_RS04140 at 820.694 kb on - strand, within LU632_RS04140 at 821.527 kb on - strand, within istA at 822.988 kb on + strand, within istB at 823.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 820,010 + LU632_RS04130 0.38 -0.6 820,010 + LU632_RS04130 0.38 +0.4 820,011 - LU632_RS04130 0.38 -0.5 820,011 - LU632_RS04130 0.38 -0.8 820,140 + LU632_RS04130 0.74 +0.9 820,140 + LU632_RS04130 0.74 -0.6 820,140 + LU632_RS04130 0.74 +0.8 820,141 - LU632_RS04130 0.74 -0.3 820,191 - LU632_RS04130 0.88 -0.4 820,194 + LU632_RS04130 0.89 -0.1 820,195 - LU632_RS04130 0.89 -2.1 820,253 + -0.3 820,254 - +0.3 820,292 + +0.0 820,292 + -0.6 820,354 - LU632_RS04135 0.39 +0.0 820,375 - LU632_RS04135 0.51 +1.7 820,446 + +0.4 820,455 - -0.3 820,496 + LU632_RS04140 0.13 +0.5 820,694 - LU632_RS04140 0.77 -1.6 821,527 - istA LU632_RS04145 0.35 -0.6 822,988 + istB LU632_RS04150 0.60 +1.2 823,392 - +0.0
Or see this region's nucleotide sequence