Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04115 and radC are separated by 11 nucleotides radC and LU632_RS04125 are separated by 12 nucleotides LU632_RS04125 and LU632_RS04130 are separated by 22 nucleotides LU632_RS04130 and LU632_RS04135 are separated by 52 nucleotides LU632_RS04135 and LU632_RS04140 overlap by 7 nucleotides LU632_RS04140 and istA are separated by 231 nucleotides
LU632_RS04115: LU632_RS04115 - antirestriction protein, at 818,646 to 819,125
_RS04115
LU632_RS04120: radC - DNA repair protein RadC, at 819,137 to 819,616
radC
LU632_RS04125: LU632_RS04125 - DUF987 domain-containing protein, at 819,629 to 819,850
_RS04125
LU632_RS04130: LU632_RS04130 - type IV toxin-antitoxin system YeeU family antitoxin, at 819,873 to 820,232
_RS04130
LU632_RS04135: LU632_RS04135 - toxin, at 820,285 to 820,461
_RS04135
LU632_RS04140: LU632_RS04140 - terminase family protein, at 820,455 to 820,766
_RS04140
LU632_RS04145: istA - IS21 family transposase, at 820,998 to 822,527
istA
Position (kb) 819
820
821 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 818.921 kb on + strand, within LU632_RS04115 at 818.922 kb on - strand, within LU632_RS04115 at 818.922 kb on - strand, within LU632_RS04115 at 818.922 kb on - strand, within LU632_RS04115 at 818.926 kb on + strand, within LU632_RS04115 at 819.007 kb on + strand, within LU632_RS04115 at 819.007 kb on + strand, within LU632_RS04115 at 819.057 kb on + strand, within LU632_RS04115 at 819.057 kb on + strand, within LU632_RS04115 at 819.057 kb on + strand, within LU632_RS04115 at 819.156 kb on + strand at 819.171 kb on + strand at 819.228 kb on + strand, within radC at 819.229 kb on - strand, within radC at 819.352 kb on + strand, within radC at 819.353 kb on - strand, within radC at 819.624 kb on + strand at 819.663 kb on + strand, within LU632_RS04125 at 819.664 kb on - strand, within LU632_RS04125 at 819.713 kb on + strand, within LU632_RS04125 at 819.818 kb on + strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.849 kb on - strand at 819.854 kb on - strand at 819.869 kb on + strand at 819.890 kb on + strand at 819.990 kb on + strand, within LU632_RS04130 at 819.991 kb on - strand, within LU632_RS04130 at 820.010 kb on + strand, within LU632_RS04130 at 820.010 kb on + strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.141 kb on - strand, within LU632_RS04130 at 820.191 kb on - strand, within LU632_RS04130 at 820.194 kb on + strand, within LU632_RS04130 at 820.195 kb on - strand, within LU632_RS04130 at 820.253 kb on + strand at 820.254 kb on - strand at 820.292 kb on + strand at 820.292 kb on + strand at 820.354 kb on - strand, within LU632_RS04135 at 820.375 kb on - strand, within LU632_RS04135 at 820.446 kb on + strand at 820.455 kb on - strand at 820.496 kb on + strand, within LU632_RS04140 at 820.694 kb on - strand, within LU632_RS04140
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 818,921 + LU632_RS04115 0.57 -0.6 818,922 - LU632_RS04115 0.57 -0.1 818,922 - LU632_RS04115 0.57 -0.1 818,922 - LU632_RS04115 0.57 +0.9 818,926 + LU632_RS04115 0.58 +0.0 819,007 + LU632_RS04115 0.75 +0.3 819,007 + LU632_RS04115 0.75 +1.4 819,057 + LU632_RS04115 0.86 +0.5 819,057 + LU632_RS04115 0.86 +0.6 819,057 + LU632_RS04115 0.86 +0.4 819,156 + +1.5 819,171 + -0.4 819,228 + radC LU632_RS04120 0.19 +0.2 819,229 - radC LU632_RS04120 0.19 +0.5 819,352 + radC LU632_RS04120 0.45 +2.8 819,353 - radC LU632_RS04120 0.45 -0.1 819,624 + +0.1 819,663 + LU632_RS04125 0.15 -0.1 819,664 - LU632_RS04125 0.16 +0.0 819,713 + LU632_RS04125 0.38 -0.5 819,818 + LU632_RS04125 0.85 +0.4 819,819 - LU632_RS04125 0.86 -0.6 819,819 - LU632_RS04125 0.86 -1.1 819,819 - LU632_RS04125 0.86 -0.5 819,819 - LU632_RS04125 0.86 +0.2 819,849 - -0.5 819,854 - +1.5 819,869 + -0.6 819,890 + +0.1 819,990 + LU632_RS04130 0.33 -0.3 819,991 - LU632_RS04130 0.33 -0.4 820,010 + LU632_RS04130 0.38 -0.6 820,010 + LU632_RS04130 0.38 +0.4 820,011 - LU632_RS04130 0.38 -0.5 820,011 - LU632_RS04130 0.38 -0.8 820,140 + LU632_RS04130 0.74 +0.9 820,140 + LU632_RS04130 0.74 -0.6 820,140 + LU632_RS04130 0.74 +0.8 820,141 - LU632_RS04130 0.74 -0.3 820,191 - LU632_RS04130 0.88 -0.4 820,194 + LU632_RS04130 0.89 -0.1 820,195 - LU632_RS04130 0.89 -2.1 820,253 + -0.3 820,254 - +0.3 820,292 + +0.0 820,292 + -0.6 820,354 - LU632_RS04135 0.39 +0.0 820,375 - LU632_RS04135 0.51 +1.7 820,446 + +0.4 820,455 - -0.3 820,496 + LU632_RS04140 0.13 +0.5 820,694 - LU632_RS04140 0.77 -1.6
Or see this region's nucleotide sequence