Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS03220 and sctL overlap by 7 nucleotides sctL and LU632_RS03230 are separated by 86 nucleotides LU632_RS03230 and LU632_RS03235 overlap by 14 nucleotides LU632_RS03235 and sctC overlap by 8 nucleotides
LU632_RS03220: LU632_RS03220 - hypothetical protein, at 658,242 to 658,832
_RS03220
LU632_RS03225: sctL - type III secretion system stator protein SctL, at 658,826 to 659,410
sctL
LU632_RS03230: LU632_RS03230 - type III secretion system protein, at 659,497 to 659,733
_RS03230
LU632_RS03235: LU632_RS03235 - type III secretion system protein, at 659,720 to 660,151
_RS03235
LU632_RS03240: sctC - type III secretion system outer membrane ring subunit SctC, at 660,144 to 662,168
sctC
Position (kb) 659
660
661 Strain fitness (log2 ratio) -1
0
1 at 658.825 kb on + strand at 658.924 kb on + strand, within sctL at 658.925 kb on - strand, within sctL at 658.925 kb on - strand, within sctL at 658.927 kb on - strand, within sctL at 659.360 kb on + strand at 659.361 kb on - strand at 659.413 kb on - strand at 659.413 kb on - strand at 659.464 kb on - strand at 659.464 kb on - strand at 659.464 kb on - strand at 659.491 kb on - strand at 659.496 kb on + strand at 659.497 kb on - strand at 659.497 kb on - strand at 659.497 kb on - strand at 659.715 kb on + strand at 659.716 kb on - strand at 659.719 kb on - strand at 659.815 kb on - strand, within LU632_RS03235 at 659.872 kb on + strand, within LU632_RS03235 at 659.881 kb on + strand, within LU632_RS03235 at 659.898 kb on + strand, within LU632_RS03235 at 659.898 kb on + strand, within LU632_RS03235 at 659.898 kb on + strand, within LU632_RS03235 at 659.919 kb on + strand, within LU632_RS03235 at 659.919 kb on + strand, within LU632_RS03235 at 659.973 kb on + strand, within LU632_RS03235 at 659.974 kb on - strand, within LU632_RS03235 at 659.974 kb on - strand, within LU632_RS03235 at 659.983 kb on - strand, within LU632_RS03235 at 659.999 kb on - strand, within LU632_RS03235 at 660.127 kb on - strand at 660.130 kb on - strand at 660.158 kb on + strand at 660.158 kb on + strand at 660.158 kb on + strand at 660.158 kb on + strand at 660.158 kb on + strand at 660.159 kb on - strand at 660.159 kb on - strand at 660.253 kb on - strand at 660.258 kb on + strand at 660.272 kb on + strand at 660.289 kb on + strand at 660.450 kb on + strand, within sctC at 660.450 kb on + strand, within sctC at 660.450 kb on + strand, within sctC at 660.451 kb on - strand, within sctC at 660.451 kb on - strand, within sctC at 660.451 kb on - strand, within sctC at 660.615 kb on + strand, within sctC at 660.616 kb on - strand, within sctC at 660.639 kb on - strand, within sctC at 660.696 kb on + strand, within sctC at 660.697 kb on - strand, within sctC at 660.697 kb on - strand, within sctC at 660.697 kb on - strand, within sctC at 660.848 kb on + strand, within sctC at 660.848 kb on + strand, within sctC at 660.849 kb on - strand, within sctC at 660.899 kb on + strand, within sctC at 660.983 kb on - strand, within sctC at 660.985 kb on + strand, within sctC at 661.001 kb on + strand, within sctC at 661.001 kb on + strand, within sctC at 661.001 kb on + strand, within sctC at 661.002 kb on - strand, within sctC at 661.017 kb on + strand, within sctC at 661.028 kb on + strand, within sctC at 661.028 kb on + strand, within sctC at 661.136 kb on - strand, within sctC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 658,825 + -0.4 658,924 + sctL LU632_RS03225 0.17 +0.1 658,925 - sctL LU632_RS03225 0.17 -0.3 658,925 - sctL LU632_RS03225 0.17 -1.0 658,927 - sctL LU632_RS03225 0.17 +0.0 659,360 + -0.1 659,361 - -0.2 659,413 - -0.8 659,413 - +0.6 659,464 - +0.7 659,464 - -0.6 659,464 - +0.3 659,491 - -1.4 659,496 + +0.8 659,497 - +0.4 659,497 - -0.1 659,497 - +0.1 659,715 + +0.3 659,716 - +1.0 659,719 - +0.2 659,815 - LU632_RS03235 0.22 +1.4 659,872 + LU632_RS03235 0.35 +0.0 659,881 + LU632_RS03235 0.37 -0.2 659,898 + LU632_RS03235 0.41 +0.7 659,898 + LU632_RS03235 0.41 +0.7 659,898 + LU632_RS03235 0.41 +0.8 659,919 + LU632_RS03235 0.46 -0.0 659,919 + LU632_RS03235 0.46 +1.1 659,973 + LU632_RS03235 0.59 +0.3 659,974 - LU632_RS03235 0.59 -0.0 659,974 - LU632_RS03235 0.59 -0.0 659,983 - LU632_RS03235 0.61 +0.2 659,999 - LU632_RS03235 0.65 +0.2 660,127 - -0.9 660,130 - +0.1 660,158 + -0.3 660,158 + -0.3 660,158 + -0.1 660,158 + +0.5 660,158 + +0.4 660,159 - +0.1 660,159 - -0.2 660,253 - +0.7 660,258 + -0.5 660,272 + -0.8 660,289 + +0.7 660,450 + sctC LU632_RS03240 0.15 +1.2 660,450 + sctC LU632_RS03240 0.15 -0.4 660,450 + sctC LU632_RS03240 0.15 +1.0 660,451 - sctC LU632_RS03240 0.15 +0.3 660,451 - sctC LU632_RS03240 0.15 +0.3 660,451 - sctC LU632_RS03240 0.15 -1.6 660,615 + sctC LU632_RS03240 0.23 +0.0 660,616 - sctC LU632_RS03240 0.23 -0.2 660,639 - sctC LU632_RS03240 0.24 +0.2 660,696 + sctC LU632_RS03240 0.27 +0.5 660,697 - sctC LU632_RS03240 0.27 +0.7 660,697 - sctC LU632_RS03240 0.27 -0.5 660,697 - sctC LU632_RS03240 0.27 +0.7 660,848 + sctC LU632_RS03240 0.35 +0.3 660,848 + sctC LU632_RS03240 0.35 +1.0 660,849 - sctC LU632_RS03240 0.35 +0.3 660,899 + sctC LU632_RS03240 0.37 -0.4 660,983 - sctC LU632_RS03240 0.41 -0.6 660,985 + sctC LU632_RS03240 0.42 -0.4 661,001 + sctC LU632_RS03240 0.42 -0.8 661,001 + sctC LU632_RS03240 0.42 +0.4 661,001 + sctC LU632_RS03240 0.42 -0.7 661,002 - sctC LU632_RS03240 0.42 -0.2 661,017 + sctC LU632_RS03240 0.43 +0.6 661,028 + sctC LU632_RS03240 0.44 +1.0 661,028 + sctC LU632_RS03240 0.44 -0.4 661,136 - sctC LU632_RS03240 0.49 +1.0
Or see this region's nucleotide sequence