Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt orn and LU632_RS01955 are separated by 173 nucleotides LU632_RS01955 and LU632_RS01960 are separated by 572 nucleotides LU632_RS01960 and LU632_RS01965 are separated by 473 nucleotides
LU632_RS01950: orn - oligoribonuclease, at 374,346 to 374,891
orn
LU632_RS01955: LU632_RS01955 - tRNA-Gly, at 375,065 to 375,140
_RS01955
LU632_RS01960: LU632_RS01960 - winged helix-turn-helix domain-containing protein, at 375,713 to 376,162
_RS01960
LU632_RS01965: LU632_RS01965 - type 1 fimbrial protein, at 376,636 to 377,217
_RS01965
Position (kb)
375
376
377 Strain fitness (log2 ratio)
-1
0
1 at 374.889 kb on + strand at 375.012 kb on - strand at 375.017 kb on + strand at 375.018 kb on - strand at 375.019 kb on + strand at 375.020 kb on - strand at 375.057 kb on + strand at 375.154 kb on + strand at 375.155 kb on - strand at 375.155 kb on - strand at 375.156 kb on + strand at 375.156 kb on + strand at 375.218 kb on - strand at 375.280 kb on + strand at 375.378 kb on + strand at 375.387 kb on - strand at 375.488 kb on - strand at 375.984 kb on + strand, within LU632_RS01960 at 376.471 kb on + strand at 376.566 kb on + strand at 376.670 kb on + strand at 376.725 kb on + strand, within LU632_RS01965 at 376.740 kb on + strand, within LU632_RS01965 at 376.762 kb on - strand, within LU632_RS01965 at 376.811 kb on + strand, within LU632_RS01965 at 376.881 kb on + strand, within LU632_RS01965 at 376.986 kb on + strand, within LU632_RS01965 at 376.987 kb on - strand, within LU632_RS01965 at 377.079 kb on - strand, within LU632_RS01965 at 377.143 kb on + strand, within LU632_RS01965 at 377.144 kb on - strand, within LU632_RS01965
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 374,889 + +0.6 375,012 - +0.1 375,017 + +0.1 375,018 - -0.7 375,019 + -0.7 375,020 - -0.0 375,057 + -0.7 375,154 + +0.2 375,155 - -0.4 375,155 - +0.7 375,156 + +1.5 375,156 + +0.4 375,218 - -0.3 375,280 + -0.2 375,378 + -0.1 375,387 - +0.6 375,488 - -0.1 375,984 + LU632_RS01960 0.60 +0.3 376,471 + -1.6 376,566 + -1.0 376,670 + -1.7 376,725 + LU632_RS01965 0.15 +0.3 376,740 + LU632_RS01965 0.18 +0.3 376,762 - LU632_RS01965 0.22 -0.7 376,811 + LU632_RS01965 0.30 -0.7 376,881 + LU632_RS01965 0.42 -0.3 376,986 + LU632_RS01965 0.60 -0.0 376,987 - LU632_RS01965 0.60 -0.2 377,079 - LU632_RS01965 0.76 -0.1 377,143 + LU632_RS01965 0.87 -0.6 377,144 - LU632_RS01965 0.87 -1.2
Or see this region's nucleotide sequence