Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00440 and LU632_RS00445 are separated by 300 nucleotides LU632_RS00445 and sctC overlap by 8 nucleotides
LU632_RS00440: LU632_RS00440 - PrgH/EprH family type III secretion apparatus protein, at 89,596 to 90,849
_RS00440
LU632_RS00445: LU632_RS00445 - helix-turn-helix domain-containing protein, at 91,150 to 92,028
_RS00445
LU632_RS00450: sctC - type III secretion system outer membrane ring subunit SctC, at 92,021 to 93,793
sctC
Position (kb) 91
92
93 Strain fitness (log2 ratio) -1
0
1 at 90.209 kb on - strand, within LU632_RS00440 at 90.314 kb on - strand, within LU632_RS00440 at 90.314 kb on - strand, within LU632_RS00440 at 90.372 kb on + strand, within LU632_RS00440 at 90.637 kb on - strand, within LU632_RS00440 at 90.789 kb on - strand at 91.272 kb on + strand, within LU632_RS00445 at 91.478 kb on - strand, within LU632_RS00445 at 91.553 kb on + strand, within LU632_RS00445 at 91.781 kb on - strand, within LU632_RS00445 at 91.881 kb on - strand, within LU632_RS00445 at 91.888 kb on - strand, within LU632_RS00445 at 91.900 kb on - strand, within LU632_RS00445 at 91.900 kb on - strand, within LU632_RS00445 at 92.177 kb on + strand at 92.363 kb on - strand, within sctC at 92.368 kb on + strand, within sctC at 92.378 kb on + strand, within sctC at 92.387 kb on - strand, within sctC at 92.536 kb on - strand, within sctC at 92.537 kb on + strand, within sctC at 92.537 kb on + strand, within sctC at 92.541 kb on + strand, within sctC at 92.568 kb on - strand, within sctC at 92.568 kb on - strand, within sctC at 92.569 kb on + strand, within sctC at 92.582 kb on + strand, within sctC at 92.583 kb on + strand, within sctC at 92.623 kb on + strand, within sctC at 92.657 kb on + strand, within sctC at 92.657 kb on + strand, within sctC at 92.658 kb on - strand, within sctC at 92.661 kb on + strand, within sctC at 92.662 kb on - strand, within sctC at 92.671 kb on + strand, within sctC at 92.710 kb on + strand, within sctC at 92.710 kb on + strand, within sctC at 92.722 kb on + strand, within sctC at 92.767 kb on - strand, within sctC at 92.771 kb on - strand, within sctC at 92.822 kb on + strand, within sctC at 92.905 kb on + strand, within sctC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 90,209 - LU632_RS00440 0.49 +0.2 90,314 - LU632_RS00440 0.57 +1.1 90,314 - LU632_RS00440 0.57 -1.5 90,372 + LU632_RS00440 0.62 +1.0 90,637 - LU632_RS00440 0.83 +0.2 90,789 - +0.1 91,272 + LU632_RS00445 0.14 +0.3 91,478 - LU632_RS00445 0.37 +0.3 91,553 + LU632_RS00445 0.46 +0.1 91,781 - LU632_RS00445 0.72 +1.1 91,881 - LU632_RS00445 0.83 +0.3 91,888 - LU632_RS00445 0.84 +0.6 91,900 - LU632_RS00445 0.85 +0.4 91,900 - LU632_RS00445 0.85 +0.5 92,177 + +0.9 92,363 - sctC LU632_RS00450 0.19 +0.4 92,368 + sctC LU632_RS00450 0.20 -0.0 92,378 + sctC LU632_RS00450 0.20 +0.8 92,387 - sctC LU632_RS00450 0.21 +0.3 92,536 - sctC LU632_RS00450 0.29 +0.3 92,537 + sctC LU632_RS00450 0.29 -0.0 92,537 + sctC LU632_RS00450 0.29 +0.3 92,541 + sctC LU632_RS00450 0.29 +0.4 92,568 - sctC LU632_RS00450 0.31 +0.9 92,568 - sctC LU632_RS00450 0.31 +1.4 92,569 + sctC LU632_RS00450 0.31 +1.5 92,582 + sctC LU632_RS00450 0.32 +0.0 92,583 + sctC LU632_RS00450 0.32 +0.8 92,623 + sctC LU632_RS00450 0.34 +0.3 92,657 + sctC LU632_RS00450 0.36 -0.0 92,657 + sctC LU632_RS00450 0.36 +0.2 92,658 - sctC LU632_RS00450 0.36 +0.4 92,661 + sctC LU632_RS00450 0.36 +0.4 92,662 - sctC LU632_RS00450 0.36 +0.6 92,671 + sctC LU632_RS00450 0.37 +0.2 92,710 + sctC LU632_RS00450 0.39 +0.9 92,710 + sctC LU632_RS00450 0.39 +0.8 92,722 + sctC LU632_RS00450 0.40 -1.6 92,767 - sctC LU632_RS00450 0.42 +0.2 92,771 - sctC LU632_RS00450 0.42 -1.7 92,822 + sctC LU632_RS00450 0.45 +0.2 92,905 + sctC LU632_RS00450 0.50 +0.1
Or see this region's nucleotide sequence