Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS21640 and LU632_RS21645 overlap by 82 nucleotides LU632_RS21645 and LU632_RS21650 are separated by 92 nucleotides LU632_RS21650 and LU632_RS21655 overlap by 14 nucleotides LU632_RS21655 and ampH are separated by 603 nucleotides
LU632_RS21640: LU632_RS21640 - IS1 family transposase, at 4,138,594 to 4,139,097
_RS21640
LU632_RS21645: LU632_RS21645 - transposase, at 4,139,016 to 4,139,291
_RS21645
LU632_RS21650: LU632_RS21650 - hypothetical protein, at 4,139,384 to 4,139,737
_RS21650
LU632_RS21655: LU632_RS21655 - pesticin C-terminus-like muramidase, at 4,139,724 to 4,140,371
_RS21655
LU632_RS21660: ampH - D-alanyl-D-alanine- carboxypeptidase/endopeptidase AmpH, at 4,140,975 to 4,142,168
ampH
Position (kb)
4139
4140
4141 Strain fitness (log2 ratio)
-1
0
1 at 4140.412 kb on + strand at 4140.420 kb on + strand at 4140.469 kb on + strand at 4140.513 kb on - strand at 4140.513 kb on - strand at 4140.515 kb on - strand at 4140.613 kb on - strand at 4140.631 kb on + strand at 4140.632 kb on - strand at 4140.632 kb on - strand at 4140.650 kb on + strand at 4140.651 kb on - strand at 4140.986 kb on + strand at 4140.986 kb on + strand at 4140.989 kb on + strand at 4140.990 kb on - strand at 4141.134 kb on - strand, within ampH at 4141.139 kb on + strand, within ampH at 4141.139 kb on + strand, within ampH at 4141.139 kb on + strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.140 kb on - strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.141 kb on + strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.142 kb on - strand, within ampH at 4141.178 kb on + strand, within ampH at 4141.211 kb on - strand, within ampH
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,140,412 + +0.5 4,140,420 + -0.7 4,140,469 + +1.1 4,140,513 - +0.6 4,140,513 - +1.1 4,140,515 - -0.3 4,140,613 - -0.2 4,140,631 + -0.3 4,140,632 - +0.3 4,140,632 - -0.3 4,140,650 + -0.0 4,140,651 - -0.7 4,140,986 + -0.7 4,140,986 + -0.3 4,140,989 + +1.4 4,140,990 - +0.1 4,141,134 - ampH LU632_RS21660 0.13 -0.4 4,141,139 + ampH LU632_RS21660 0.14 +0.3 4,141,139 + ampH LU632_RS21660 0.14 -0.5 4,141,139 + ampH LU632_RS21660 0.14 +0.2 4,141,140 - ampH LU632_RS21660 0.14 +0.3 4,141,140 - ampH LU632_RS21660 0.14 -0.2 4,141,140 - ampH LU632_RS21660 0.14 -0.2 4,141,140 - ampH LU632_RS21660 0.14 -0.7 4,141,140 - ampH LU632_RS21660 0.14 +0.6 4,141,140 - ampH LU632_RS21660 0.14 +0.0 4,141,140 - ampH LU632_RS21660 0.14 -1.1 4,141,140 - ampH LU632_RS21660 0.14 +0.4 4,141,141 + ampH LU632_RS21660 0.14 -0.2 4,141,141 + ampH LU632_RS21660 0.14 +0.2 4,141,141 + ampH LU632_RS21660 0.14 +0.1 4,141,141 + ampH LU632_RS21660 0.14 -0.8 4,141,141 + ampH LU632_RS21660 0.14 +0.1 4,141,141 + ampH LU632_RS21660 0.14 +0.1 4,141,142 - ampH LU632_RS21660 0.14 +0.9 4,141,142 - ampH LU632_RS21660 0.14 -0.3 4,141,142 - ampH LU632_RS21660 0.14 +0.4 4,141,142 - ampH LU632_RS21660 0.14 -0.0 4,141,142 - ampH LU632_RS21660 0.14 +0.3 4,141,142 - ampH LU632_RS21660 0.14 +0.4 4,141,142 - ampH LU632_RS21660 0.14 +0.1 4,141,142 - ampH LU632_RS21660 0.14 -0.1 4,141,178 + ampH LU632_RS21660 0.17 -0.5 4,141,211 - ampH LU632_RS21660 0.20 -0.4
Or see this region's nucleotide sequence