Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdD and nrdG overlap by 4 nucleotides nrdG and LU632_RS20985 are separated by 5 nucleotides LU632_RS20985 and LU632_RS20990 are separated by 105 nucleotides LU632_RS20990 and LU632_RS20995 overlap by 24 nucleotides
LU632_RS20975: nrdD - anaerobic ribonucleoside-triphosphate reductase, at 4,008,905 to 4,011,043
nrdD
LU632_RS20980: nrdG - anaerobic ribonucleoside-triphosphate reductase-activating protein, at 4,011,040 to 4,011,504
nrdG
LU632_RS20985: LU632_RS20985 - YhbP family protein, at 4,011,510 to 4,011,938
_RS20985
LU632_RS20990: LU632_RS20990 - GIY-YIG nuclease family protein, at 4,012,044 to 4,012,358
_RS20990
LU632_RS20995: LU632_RS20995 - N-acetyltransferase, at 4,012,335 to 4,012,838
_RS20995
Position (kb)
4011
4012 Strain fitness (log2 ratio)
-1
0
1
2 at 4010.113 kb on + strand, within nrdD at 4010.117 kb on - strand, within nrdD at 4010.132 kb on - strand, within nrdD at 4010.276 kb on - strand, within nrdD at 4010.282 kb on + strand, within nrdD at 4010.285 kb on - strand, within nrdD at 4010.395 kb on + strand, within nrdD at 4010.426 kb on + strand, within nrdD at 4010.475 kb on + strand, within nrdD at 4010.550 kb on + strand, within nrdD at 4010.590 kb on + strand, within nrdD at 4010.593 kb on + strand, within nrdD at 4010.593 kb on + strand, within nrdD at 4010.593 kb on + strand, within nrdD at 4010.618 kb on + strand, within nrdD at 4010.618 kb on + strand, within nrdD at 4010.783 kb on + strand, within nrdD at 4010.783 kb on + strand, within nrdD at 4010.784 kb on - strand, within nrdD at 4010.784 kb on - strand, within nrdD at 4010.785 kb on + strand, within nrdD at 4010.785 kb on + strand, within nrdD at 4010.785 kb on + strand, within nrdD at 4010.789 kb on + strand, within nrdD at 4010.790 kb on - strand, within nrdD at 4010.807 kb on + strand, within nrdD at 4010.808 kb on - strand, within nrdD at 4011.001 kb on - strand at 4011.019 kb on + strand at 4011.019 kb on + strand at 4011.020 kb on - strand at 4011.020 kb on - strand at 4011.020 kb on - strand at 4011.020 kb on - strand at 4011.020 kb on - strand at 4011.036 kb on + strand at 4011.048 kb on - strand at 4011.055 kb on + strand at 4011.056 kb on - strand at 4011.139 kb on + strand, within nrdG at 4011.140 kb on - strand, within nrdG at 4011.181 kb on - strand, within nrdG at 4011.339 kb on + strand, within nrdG at 4011.340 kb on - strand, within nrdG at 4011.490 kb on + strand at 4011.497 kb on + strand at 4011.497 kb on + strand at 4011.497 kb on + strand at 4011.511 kb on + strand at 4011.511 kb on + strand at 4011.511 kb on + strand at 4011.511 kb on + strand at 4011.512 kb on - strand at 4011.551 kb on + strand at 4011.552 kb on - strand at 4011.582 kb on + strand, within LU632_RS20985 at 4011.630 kb on + strand, within LU632_RS20985 at 4011.643 kb on + strand, within LU632_RS20985 at 4011.643 kb on + strand, within LU632_RS20985 at 4011.691 kb on + strand, within LU632_RS20985 at 4011.692 kb on - strand, within LU632_RS20985 at 4011.696 kb on + strand, within LU632_RS20985 at 4011.763 kb on + strand, within LU632_RS20985 at 4011.764 kb on - strand, within LU632_RS20985 at 4011.774 kb on - strand, within LU632_RS20985 at 4011.911 kb on + strand at 4011.984 kb on + strand at 4011.984 kb on + strand at 4012.086 kb on + strand, within LU632_RS20990 at 4012.086 kb on + strand, within LU632_RS20990 at 4012.088 kb on + strand, within LU632_RS20990 at 4012.327 kb on + strand at 4012.327 kb on + strand at 4012.328 kb on - strand at 4012.336 kb on + strand at 4012.341 kb on - strand at 4012.341 kb on - strand at 4012.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,010,113 + nrdD LU632_RS20975 0.56 +0.2 4,010,117 - nrdD LU632_RS20975 0.57 +0.7 4,010,132 - nrdD LU632_RS20975 0.57 -0.4 4,010,276 - nrdD LU632_RS20975 0.64 +0.2 4,010,282 + nrdD LU632_RS20975 0.64 +0.2 4,010,285 - nrdD LU632_RS20975 0.65 +1.6 4,010,395 + nrdD LU632_RS20975 0.70 -0.3 4,010,426 + nrdD LU632_RS20975 0.71 -0.5 4,010,475 + nrdD LU632_RS20975 0.73 -0.0 4,010,550 + nrdD LU632_RS20975 0.77 +1.9 4,010,590 + nrdD LU632_RS20975 0.79 +0.9 4,010,593 + nrdD LU632_RS20975 0.79 -0.5 4,010,593 + nrdD LU632_RS20975 0.79 -1.2 4,010,593 + nrdD LU632_RS20975 0.79 -0.3 4,010,618 + nrdD LU632_RS20975 0.80 +0.0 4,010,618 + nrdD LU632_RS20975 0.80 -0.1 4,010,783 + nrdD LU632_RS20975 0.88 +1.2 4,010,783 + nrdD LU632_RS20975 0.88 -0.5 4,010,784 - nrdD LU632_RS20975 0.88 +0.2 4,010,784 - nrdD LU632_RS20975 0.88 -0.7 4,010,785 + nrdD LU632_RS20975 0.88 -0.4 4,010,785 + nrdD LU632_RS20975 0.88 -0.3 4,010,785 + nrdD LU632_RS20975 0.88 -1.1 4,010,789 + nrdD LU632_RS20975 0.88 -0.5 4,010,790 - nrdD LU632_RS20975 0.88 +0.2 4,010,807 + nrdD LU632_RS20975 0.89 +0.5 4,010,808 - nrdD LU632_RS20975 0.89 -0.7 4,011,001 - +1.5 4,011,019 + +0.1 4,011,019 + -0.4 4,011,020 - +0.4 4,011,020 - -1.1 4,011,020 - +0.2 4,011,020 - +0.1 4,011,020 - -0.3 4,011,036 + -0.9 4,011,048 - +0.3 4,011,055 + -0.6 4,011,056 - +0.1 4,011,139 + nrdG LU632_RS20980 0.21 -0.1 4,011,140 - nrdG LU632_RS20980 0.22 +0.4 4,011,181 - nrdG LU632_RS20980 0.30 +1.5 4,011,339 + nrdG LU632_RS20980 0.64 +1.2 4,011,340 - nrdG LU632_RS20980 0.65 +1.5 4,011,490 + +0.2 4,011,497 + -0.1 4,011,497 + -0.4 4,011,497 + -0.2 4,011,511 + -0.7 4,011,511 + +0.7 4,011,511 + +0.3 4,011,511 + +0.3 4,011,512 - -0.3 4,011,551 + +0.1 4,011,552 - +0.9 4,011,582 + LU632_RS20985 0.17 +0.6 4,011,630 + LU632_RS20985 0.28 -0.4 4,011,643 + LU632_RS20985 0.31 -0.2 4,011,643 + LU632_RS20985 0.31 -0.8 4,011,691 + LU632_RS20985 0.42 -0.3 4,011,692 - LU632_RS20985 0.42 +0.2 4,011,696 + LU632_RS20985 0.43 -1.2 4,011,763 + LU632_RS20985 0.59 +0.3 4,011,764 - LU632_RS20985 0.59 -1.4 4,011,774 - LU632_RS20985 0.62 -0.3 4,011,911 + -0.2 4,011,984 + -0.1 4,011,984 + -0.3 4,012,086 + LU632_RS20990 0.13 -0.3 4,012,086 + LU632_RS20990 0.13 +0.1 4,012,088 + LU632_RS20990 0.14 +0.2 4,012,327 + -0.1 4,012,327 + +0.9 4,012,328 - +0.3 4,012,336 + +0.2 4,012,341 - -0.2 4,012,341 - +0.2 4,012,361 - +0.5
Or see this region's nucleotide sequence