Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and pyrI are separated by 10 nucleotides pyrI and ridA are separated by 120 nucleotides ridA and LU632_RS20965 are separated by 201 nucleotides
LU632_RS20950: pyrB - aspartate carbamoyltransferase, at 4,002,284 to 4,003,219
pyrB
LU632_RS20955: pyrI - aspartate carbamoyltransferase regulatory subunit, at 4,003,230 to 4,003,694
pyrI
LU632_RS20960: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 4,003,815 to 4,004,201
ridA
LU632_RS20965: LU632_RS20965 - alpha,alpha-phosphotrehalase, at 4,004,403 to 4,006,082
_RS20965
Position (kb)
4003
4004
4005 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4002.875 kb on + strand, within pyrB at 4002.876 kb on - strand, within pyrB at 4002.876 kb on - strand, within pyrB at 4002.876 kb on - strand, within pyrB at 4002.876 kb on - strand, within pyrB at 4002.878 kb on - strand, within pyrB at 4002.962 kb on + strand, within pyrB at 4002.962 kb on + strand, within pyrB at 4002.962 kb on + strand, within pyrB at 4002.965 kb on - strand, within pyrB at 4003.004 kb on + strand, within pyrB at 4003.077 kb on + strand, within pyrB at 4003.078 kb on - strand, within pyrB at 4003.078 kb on - strand, within pyrB at 4003.078 kb on - strand, within pyrB at 4003.078 kb on - strand, within pyrB at 4003.093 kb on + strand, within pyrB at 4003.094 kb on - strand, within pyrB at 4003.140 kb on - strand at 4003.162 kb on + strand at 4003.162 kb on + strand at 4003.163 kb on - strand at 4003.166 kb on + strand at 4003.167 kb on - strand at 4003.167 kb on - strand at 4003.215 kb on + strand at 4003.216 kb on - strand at 4003.218 kb on - strand at 4003.244 kb on + strand at 4003.245 kb on - strand at 4003.290 kb on - strand, within pyrI at 4003.355 kb on + strand, within pyrI at 4003.402 kb on + strand, within pyrI at 4003.403 kb on - strand, within pyrI at 4003.458 kb on + strand, within pyrI at 4003.459 kb on - strand, within pyrI at 4003.459 kb on - strand, within pyrI at 4003.525 kb on + strand, within pyrI at 4003.643 kb on + strand, within pyrI at 4003.647 kb on + strand, within pyrI at 4003.692 kb on + strand at 4003.711 kb on + strand at 4003.712 kb on - strand at 4003.712 kb on - strand at 4003.714 kb on - strand at 4003.714 kb on - strand at 4003.723 kb on + strand at 4003.723 kb on + strand at 4003.814 kb on + strand at 4003.814 kb on + strand at 4003.814 kb on + strand at 4003.814 kb on + strand at 4003.815 kb on - strand at 4004.067 kb on - strand, within ridA at 4004.085 kb on + strand, within ridA at 4004.144 kb on - strand, within ridA at 4004.156 kb on + strand, within ridA at 4004.239 kb on + strand at 4004.334 kb on + strand at 4004.523 kb on + strand at 4004.549 kb on + strand at 4004.550 kb on - strand at 4004.568 kb on + strand at 4004.595 kb on + strand, within LU632_RS20965 at 4004.610 kb on + strand, within LU632_RS20965 at 4004.610 kb on + strand, within LU632_RS20965 at 4004.610 kb on + strand, within LU632_RS20965 at 4004.778 kb on + strand, within LU632_RS20965 at 4004.779 kb on - strand, within LU632_RS20965 at 4004.781 kb on + strand, within LU632_RS20965 at 4004.781 kb on + strand, within LU632_RS20965 at 4004.781 kb on + strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.782 kb on - strand, within LU632_RS20965 at 4004.783 kb on + strand, within LU632_RS20965 at 4004.816 kb on + strand, within LU632_RS20965 at 4004.817 kb on - strand, within LU632_RS20965 at 4004.819 kb on + strand, within LU632_RS20965 at 4004.877 kb on + strand, within LU632_RS20965 at 4004.878 kb on - strand, within LU632_RS20965 at 4004.878 kb on - strand, within LU632_RS20965 at 4004.880 kb on + strand, within LU632_RS20965 at 4004.880 kb on + strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.881 kb on - strand, within LU632_RS20965 at 4004.882 kb on + strand, within LU632_RS20965 at 4004.882 kb on + strand, within LU632_RS20965 at 4004.883 kb on - strand, within LU632_RS20965 at 4004.883 kb on - strand, within LU632_RS20965 at 4004.883 kb on - strand, within LU632_RS20965 at 4005.070 kb on - strand, within LU632_RS20965 at 4005.096 kb on + strand, within LU632_RS20965 at 4005.096 kb on + strand, within LU632_RS20965 at 4005.097 kb on - strand, within LU632_RS20965 at 4005.097 kb on - strand, within LU632_RS20965 at 4005.097 kb on - strand, within LU632_RS20965 at 4005.097 kb on - strand, within LU632_RS20965 at 4005.097 kb on - strand, within LU632_RS20965 at 4005.098 kb on + strand, within LU632_RS20965 at 4005.098 kb on + strand, within LU632_RS20965 at 4005.098 kb on + strand, within LU632_RS20965 at 4005.099 kb on - strand, within LU632_RS20965
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,002,875 + pyrB LU632_RS20950 0.63 +0.2 4,002,876 - pyrB LU632_RS20950 0.63 +2.4 4,002,876 - pyrB LU632_RS20950 0.63 -0.5 4,002,876 - pyrB LU632_RS20950 0.63 -0.2 4,002,876 - pyrB LU632_RS20950 0.63 -0.2 4,002,878 - pyrB LU632_RS20950 0.63 -0.5 4,002,962 + pyrB LU632_RS20950 0.72 -0.1 4,002,962 + pyrB LU632_RS20950 0.72 +0.3 4,002,962 + pyrB LU632_RS20950 0.72 -1.6 4,002,965 - pyrB LU632_RS20950 0.73 +0.9 4,003,004 + pyrB LU632_RS20950 0.77 +0.3 4,003,077 + pyrB LU632_RS20950 0.85 +0.4 4,003,078 - pyrB LU632_RS20950 0.85 -0.7 4,003,078 - pyrB LU632_RS20950 0.85 +0.4 4,003,078 - pyrB LU632_RS20950 0.85 -1.2 4,003,078 - pyrB LU632_RS20950 0.85 +0.5 4,003,093 + pyrB LU632_RS20950 0.86 -0.5 4,003,094 - pyrB LU632_RS20950 0.87 +0.7 4,003,140 - +0.3 4,003,162 + +0.6 4,003,162 + +0.1 4,003,163 - +0.1 4,003,166 + +0.9 4,003,167 - -0.3 4,003,167 - -0.7 4,003,215 + -0.2 4,003,216 - -0.2 4,003,218 - -0.7 4,003,244 + +0.2 4,003,245 - +0.4 4,003,290 - pyrI LU632_RS20955 0.13 +0.7 4,003,355 + pyrI LU632_RS20955 0.27 -0.5 4,003,402 + pyrI LU632_RS20955 0.37 -0.8 4,003,403 - pyrI LU632_RS20955 0.37 +0.6 4,003,458 + pyrI LU632_RS20955 0.49 +0.0 4,003,459 - pyrI LU632_RS20955 0.49 -1.5 4,003,459 - pyrI LU632_RS20955 0.49 -0.5 4,003,525 + pyrI LU632_RS20955 0.63 +0.2 4,003,643 + pyrI LU632_RS20955 0.89 -0.4 4,003,647 + pyrI LU632_RS20955 0.90 -0.4 4,003,692 + +2.5 4,003,711 + +0.1 4,003,712 - +0.7 4,003,712 - -0.1 4,003,714 - +0.3 4,003,714 - +0.0 4,003,723 + -1.0 4,003,723 + -1.4 4,003,814 + -0.7 4,003,814 + -0.6 4,003,814 + -0.0 4,003,814 + +0.3 4,003,815 - +0.5 4,004,067 - ridA LU632_RS20960 0.65 +0.1 4,004,085 + ridA LU632_RS20960 0.70 +1.9 4,004,144 - ridA LU632_RS20960 0.85 +1.1 4,004,156 + ridA LU632_RS20960 0.88 -0.9 4,004,239 + -0.7 4,004,334 + -0.6 4,004,523 + +0.9 4,004,549 + -0.4 4,004,550 - +0.1 4,004,568 + +0.2 4,004,595 + LU632_RS20965 0.11 -0.2 4,004,610 + LU632_RS20965 0.12 -0.1 4,004,610 + LU632_RS20965 0.12 -0.1 4,004,610 + LU632_RS20965 0.12 -0.3 4,004,778 + LU632_RS20965 0.22 +0.4 4,004,779 - LU632_RS20965 0.22 +0.1 4,004,781 + LU632_RS20965 0.23 +0.3 4,004,781 + LU632_RS20965 0.23 +0.2 4,004,781 + LU632_RS20965 0.23 -1.9 4,004,782 - LU632_RS20965 0.23 +0.5 4,004,782 - LU632_RS20965 0.23 +0.3 4,004,782 - LU632_RS20965 0.23 -0.1 4,004,782 - LU632_RS20965 0.23 -1.0 4,004,782 - LU632_RS20965 0.23 -0.4 4,004,782 - LU632_RS20965 0.23 -0.0 4,004,782 - LU632_RS20965 0.23 +0.1 4,004,783 + LU632_RS20965 0.23 +0.2 4,004,816 + LU632_RS20965 0.25 +0.3 4,004,817 - LU632_RS20965 0.25 +0.8 4,004,819 + LU632_RS20965 0.25 -0.1 4,004,877 + LU632_RS20965 0.28 +0.5 4,004,878 - LU632_RS20965 0.28 -0.2 4,004,878 - LU632_RS20965 0.28 +0.6 4,004,880 + LU632_RS20965 0.28 +0.0 4,004,880 + LU632_RS20965 0.28 -0.7 4,004,881 - LU632_RS20965 0.28 -0.4 4,004,881 - LU632_RS20965 0.28 -0.1 4,004,881 - LU632_RS20965 0.28 +0.1 4,004,881 - LU632_RS20965 0.28 +1.6 4,004,881 - LU632_RS20965 0.28 -2.2 4,004,881 - LU632_RS20965 0.28 -0.3 4,004,881 - LU632_RS20965 0.28 +0.5 4,004,881 - LU632_RS20965 0.28 +0.9 4,004,881 - LU632_RS20965 0.28 -0.4 4,004,881 - LU632_RS20965 0.28 +0.0 4,004,881 - LU632_RS20965 0.28 +0.3 4,004,882 + LU632_RS20965 0.29 +0.1 4,004,882 + LU632_RS20965 0.29 -0.1 4,004,883 - LU632_RS20965 0.29 -0.3 4,004,883 - LU632_RS20965 0.29 +0.0 4,004,883 - LU632_RS20965 0.29 +0.2 4,005,070 - LU632_RS20965 0.40 +0.2 4,005,096 + LU632_RS20965 0.41 -0.9 4,005,096 + LU632_RS20965 0.41 -0.1 4,005,097 - LU632_RS20965 0.41 +0.5 4,005,097 - LU632_RS20965 0.41 -0.9 4,005,097 - LU632_RS20965 0.41 -0.3 4,005,097 - LU632_RS20965 0.41 -1.3 4,005,097 - LU632_RS20965 0.41 +0.2 4,005,098 + LU632_RS20965 0.41 -0.4 4,005,098 + LU632_RS20965 0.41 -0.1 4,005,098 + LU632_RS20965 0.41 -0.7 4,005,099 - LU632_RS20965 0.41 -0.7
Or see this region's nucleotide sequence