Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdF and proV are separated by 377 nucleotides proV and proW overlap by 8 nucleotides
LU632_RS16815: nrdF - class 1b ribonucleoside-diphosphate reductase subunit beta, at 3,166,830 to 3,167,789
nrdF
LU632_RS16820: proV - glycine betaine/L-proline ABC transporter ATP-binding protein ProV, at 3,168,167 to 3,169,366
proV
LU632_RS16825: proW - glycine betaine/L-proline ABC transporter permease ProW, at 3,169,359 to 3,170,522
proW
Position (kb)
3168
3169
3170 Strain fitness (log2 ratio)
-1
0
1
2 at 3167.193 kb on + strand, within nrdF at 3167.193 kb on + strand, within nrdF at 3167.193 kb on + strand, within nrdF at 3167.193 kb on + strand, within nrdF at 3167.194 kb on - strand, within nrdF at 3167.229 kb on - strand, within nrdF at 3167.229 kb on - strand, within nrdF at 3167.229 kb on - strand, within nrdF at 3167.229 kb on - strand, within nrdF at 3167.254 kb on - strand, within nrdF at 3167.257 kb on - strand, within nrdF at 3167.317 kb on - strand, within nrdF at 3167.328 kb on + strand, within nrdF at 3167.410 kb on + strand, within nrdF at 3167.418 kb on + strand, within nrdF at 3167.421 kb on + strand, within nrdF at 3167.421 kb on + strand, within nrdF at 3167.422 kb on - strand, within nrdF at 3167.422 kb on - strand, within nrdF at 3167.431 kb on - strand, within nrdF at 3167.433 kb on - strand, within nrdF at 3167.504 kb on - strand, within nrdF at 3167.513 kb on - strand, within nrdF at 3167.515 kb on + strand, within nrdF at 3167.515 kb on + strand, within nrdF at 3167.516 kb on - strand, within nrdF at 3167.516 kb on - strand, within nrdF at 3167.516 kb on - strand, within nrdF at 3167.516 kb on - strand, within nrdF at 3167.551 kb on - strand, within nrdF at 3167.597 kb on + strand, within nrdF at 3167.598 kb on - strand, within nrdF at 3167.598 kb on - strand, within nrdF at 3167.598 kb on - strand, within nrdF at 3167.606 kb on + strand, within nrdF at 3167.653 kb on + strand, within nrdF at 3167.759 kb on - strand at 3167.867 kb on - strand at 3167.950 kb on - strand at 3168.089 kb on - strand at 3168.157 kb on + strand at 3168.333 kb on + strand, within proV at 3168.450 kb on + strand, within proV at 3168.538 kb on - strand, within proV at 3168.564 kb on - strand, within proV at 3168.613 kb on - strand, within proV at 3168.613 kb on - strand, within proV at 3168.721 kb on - strand, within proV at 3168.721 kb on - strand, within proV at 3168.723 kb on + strand, within proV at 3168.723 kb on + strand, within proV at 3168.723 kb on + strand, within proV at 3168.723 kb on + strand, within proV at 3168.822 kb on + strand, within proV at 3168.823 kb on - strand, within proV at 3168.856 kb on + strand, within proV at 3168.871 kb on - strand, within proV at 3168.944 kb on + strand, within proV at 3168.949 kb on - strand, within proV at 3168.949 kb on - strand, within proV at 3168.952 kb on + strand, within proV at 3168.953 kb on - strand, within proV at 3168.953 kb on - strand, within proV at 3168.970 kb on - strand, within proV at 3168.973 kb on + strand, within proV at 3169.001 kb on - strand, within proV at 3169.034 kb on - strand, within proV at 3169.298 kb on + strand at 3169.299 kb on - strand at 3169.299 kb on - strand at 3169.299 kb on - strand at 3169.300 kb on + strand at 3169.301 kb on - strand at 3169.301 kb on - strand at 3169.315 kb on + strand at 3169.327 kb on - strand at 3169.330 kb on - strand at 3169.330 kb on - strand at 3169.330 kb on - strand at 3169.338 kb on + strand at 3169.610 kb on + strand, within proW at 3169.610 kb on + strand, within proW at 3169.614 kb on - strand, within proW at 3169.631 kb on + strand, within proW at 3169.731 kb on + strand, within proW at 3169.731 kb on + strand, within proW at 3169.731 kb on + strand, within proW at 3169.731 kb on + strand, within proW at 3169.732 kb on - strand, within proW at 3169.732 kb on - strand, within proW at 3169.804 kb on + strand, within proW at 3169.991 kb on + strand, within proW at 3169.991 kb on + strand, within proW at 3169.991 kb on + strand, within proW at 3169.991 kb on + strand, within proW at 3169.991 kb on + strand, within proW at 3169.992 kb on - strand, within proW at 3169.992 kb on - strand, within proW at 3170.096 kb on - strand, within proW at 3170.102 kb on + strand, within proW at 3170.103 kb on - strand, within proW at 3170.103 kb on - strand, within proW at 3170.122 kb on + strand, within proW at 3170.122 kb on + strand, within proW at 3170.126 kb on - strand, within proW at 3170.126 kb on - strand, within proW at 3170.144 kb on + strand, within proW at 3170.148 kb on - strand, within proW at 3170.261 kb on + strand, within proW
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,167,193 + nrdF LU632_RS16815 0.38 -0.4 3,167,193 + nrdF LU632_RS16815 0.38 -0.2 3,167,193 + nrdF LU632_RS16815 0.38 +0.2 3,167,193 + nrdF LU632_RS16815 0.38 +1.0 3,167,194 - nrdF LU632_RS16815 0.38 -0.7 3,167,229 - nrdF LU632_RS16815 0.42 +0.0 3,167,229 - nrdF LU632_RS16815 0.42 -0.7 3,167,229 - nrdF LU632_RS16815 0.42 -0.0 3,167,229 - nrdF LU632_RS16815 0.42 -1.1 3,167,254 - nrdF LU632_RS16815 0.44 +0.7 3,167,257 - nrdF LU632_RS16815 0.44 +0.8 3,167,317 - nrdF LU632_RS16815 0.51 +0.6 3,167,328 + nrdF LU632_RS16815 0.52 +0.4 3,167,410 + nrdF LU632_RS16815 0.60 +0.2 3,167,418 + nrdF LU632_RS16815 0.61 +0.4 3,167,421 + nrdF LU632_RS16815 0.62 +0.2 3,167,421 + nrdF LU632_RS16815 0.62 -0.2 3,167,422 - nrdF LU632_RS16815 0.62 +1.0 3,167,422 - nrdF LU632_RS16815 0.62 +0.0 3,167,431 - nrdF LU632_RS16815 0.63 +0.0 3,167,433 - nrdF LU632_RS16815 0.63 -1.5 3,167,504 - nrdF LU632_RS16815 0.70 -0.2 3,167,513 - nrdF LU632_RS16815 0.71 -0.8 3,167,515 + nrdF LU632_RS16815 0.71 -1.0 3,167,515 + nrdF LU632_RS16815 0.71 -0.1 3,167,516 - nrdF LU632_RS16815 0.71 +0.6 3,167,516 - nrdF LU632_RS16815 0.71 +0.3 3,167,516 - nrdF LU632_RS16815 0.71 -0.9 3,167,516 - nrdF LU632_RS16815 0.71 +0.6 3,167,551 - nrdF LU632_RS16815 0.75 +0.2 3,167,597 + nrdF LU632_RS16815 0.80 -0.1 3,167,598 - nrdF LU632_RS16815 0.80 +0.0 3,167,598 - nrdF LU632_RS16815 0.80 -0.0 3,167,598 - nrdF LU632_RS16815 0.80 -1.0 3,167,606 + nrdF LU632_RS16815 0.81 +1.6 3,167,653 + nrdF LU632_RS16815 0.86 +1.6 3,167,759 - +0.7 3,167,867 - -0.7 3,167,950 - +0.7 3,168,089 - +0.3 3,168,157 + +0.1 3,168,333 + proV LU632_RS16820 0.14 -0.2 3,168,450 + proV LU632_RS16820 0.24 -1.1 3,168,538 - proV LU632_RS16820 0.31 -1.3 3,168,564 - proV LU632_RS16820 0.33 +0.7 3,168,613 - proV LU632_RS16820 0.37 -0.6 3,168,613 - proV LU632_RS16820 0.37 +0.2 3,168,721 - proV LU632_RS16820 0.46 -0.7 3,168,721 - proV LU632_RS16820 0.46 +1.0 3,168,723 + proV LU632_RS16820 0.46 +0.2 3,168,723 + proV LU632_RS16820 0.46 +0.3 3,168,723 + proV LU632_RS16820 0.46 -0.1 3,168,723 + proV LU632_RS16820 0.46 +0.3 3,168,822 + proV LU632_RS16820 0.55 +0.2 3,168,823 - proV LU632_RS16820 0.55 +0.8 3,168,856 + proV LU632_RS16820 0.57 -0.5 3,168,871 - proV LU632_RS16820 0.59 +0.3 3,168,944 + proV LU632_RS16820 0.65 +0.6 3,168,949 - proV LU632_RS16820 0.65 +0.2 3,168,949 - proV LU632_RS16820 0.65 +0.3 3,168,952 + proV LU632_RS16820 0.65 -0.1 3,168,953 - proV LU632_RS16820 0.66 +0.7 3,168,953 - proV LU632_RS16820 0.66 +0.6 3,168,970 - proV LU632_RS16820 0.67 +1.0 3,168,973 + proV LU632_RS16820 0.67 -0.6 3,169,001 - proV LU632_RS16820 0.69 -0.1 3,169,034 - proV LU632_RS16820 0.72 -0.0 3,169,298 + +0.1 3,169,299 - -0.3 3,169,299 - +1.0 3,169,299 - +1.3 3,169,300 + +0.3 3,169,301 - -0.6 3,169,301 - -0.5 3,169,315 + -1.3 3,169,327 - -0.5 3,169,330 - -0.2 3,169,330 - +0.5 3,169,330 - -0.5 3,169,338 + -0.3 3,169,610 + proW LU632_RS16825 0.22 +0.3 3,169,610 + proW LU632_RS16825 0.22 -0.1 3,169,614 - proW LU632_RS16825 0.22 -1.6 3,169,631 + proW LU632_RS16825 0.23 +0.7 3,169,731 + proW LU632_RS16825 0.32 -0.1 3,169,731 + proW LU632_RS16825 0.32 -0.3 3,169,731 + proW LU632_RS16825 0.32 -0.4 3,169,731 + proW LU632_RS16825 0.32 +1.0 3,169,732 - proW LU632_RS16825 0.32 +0.4 3,169,732 - proW LU632_RS16825 0.32 -0.1 3,169,804 + proW LU632_RS16825 0.38 +0.4 3,169,991 + proW LU632_RS16825 0.54 -0.2 3,169,991 + proW LU632_RS16825 0.54 +0.4 3,169,991 + proW LU632_RS16825 0.54 +0.5 3,169,991 + proW LU632_RS16825 0.54 -0.2 3,169,991 + proW LU632_RS16825 0.54 -0.6 3,169,992 - proW LU632_RS16825 0.54 -0.6 3,169,992 - proW LU632_RS16825 0.54 +0.1 3,170,096 - proW LU632_RS16825 0.63 +1.8 3,170,102 + proW LU632_RS16825 0.64 -1.0 3,170,103 - proW LU632_RS16825 0.64 -1.1 3,170,103 - proW LU632_RS16825 0.64 -0.1 3,170,122 + proW LU632_RS16825 0.66 -0.5 3,170,122 + proW LU632_RS16825 0.66 +0.6 3,170,126 - proW LU632_RS16825 0.66 +0.1 3,170,126 - proW LU632_RS16825 0.66 +0.1 3,170,144 + proW LU632_RS16825 0.67 -0.4 3,170,148 - proW LU632_RS16825 0.68 +1.3 3,170,261 + proW LU632_RS16825 0.77 -0.6
Or see this region's nucleotide sequence